Fig 1: Experimental platform and workflow for assessment of biological impact of mainstream cigarette smoke. A BEAS 2B cells were cultured into transwells (Corning 3402) as described in the Materials& Methods. The cells were then transferred to exposure chambers for smoke exposure. B Cigarette smoke was generated in a Borgwaldt RM20S smoking machine under ISO3308 smoking regime and diluted cigarette smoke from a reference cigarette (3R4F) was introduced into cell exposure chanbers. C After treatment with the diluted cigarette smoke for 1 h, cell viability assessed by NRU assays and testing the TEER values. NRU: neutral red uptake; TEER: trans-endothelial electrical resistance. The images of Exposure chambers and Borgwaldt RM20S smoking machine are taken from Adamson et al.52.