24 mm Transwell® with 3 µm Pore Polyester Membrane Insert, Sterile from Corning Life Sciences

Product Specs
Item24 mm Transwell® with 3 µm Pore Polyester Membrane Insert, Sterile
CompanyCorning Life Sciences
Price Pricing Info Supplier Page View Company Product Page
Catalog Number3452
Quantity6 / Pk
Brand/TrademarkCorning®
DimensionsMembrane Thickness: 10 µm, Membrane Diameter: 24 mm
Format6-well
VolumeRecommended Medium Well Volume: 2.6 mL
AreaCell Growth Area: 4.67 cm²
Well ShapeRound
ColorPlate Color: Clear, Well Bottom Color: Clear
Pore Size3 µm
SterilitySterile
TypeInserts

Specifications/Features

Lids Included: Yes

Membrane Material: Polyethylene terephthalate (PET)

Well Bottom: Flat

Surface Treatment: TC-Treated

Nominal Pore Density: 2x10⁶ pores per cm²

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Corning Life Sciences

836 North Street

Building 300 Suite 3401

Tewksbury, MA 01876

United States

Phone: (978) 442-2200
Phone: (800) 492-1110

Email: clscustserv@corning.com

Company Profile

Website: www.corning.com/lifesciences

(1) Pericytes protect rats and mice from sepsis-induced injuries by maintaining vascular reactivity and barrier function: implication of miRNAs and microvesiclesMilitary Medical ResearchMarch 13, 2023Zi-Sen Zhang, Yi-Yan Liu, Shuang-Shuang He, Dai-Qin Bao, Hong-Chen Wang, Jie Zhang, Xiao-Yong Peng, Jia-Tao Zang, Yu Zhu, Yue Wu, et al.passage) were plated on collagen-coated polyethylene terephthalate cell culture inserts (3 μm pore, 3452, Corning) in 24-well culture plates. The lower compartment of the Transwell was filled with 600 μl of

(2) Renal Endothelial Cell‐Targeted Extracellular Vesicles Protect the Kidney from Ischemic InjuryAdvanced ScienceJanuary 1, 2023Kaiyue Zhang, Rongrong Li, Xiaoniao Chen, Hongyu Yan, Huifang Li, Xiaotong Zhao, Haoyan Huang, Shang Chen, Yue Liu, Kai Wang, et al.used for the cell cycle assay were cultured in the lower chamber of the modified Transwell system (3452, Corning) and subjected to H/R. EVs or PBP‐EVs (100 µg mL−1) were added to the upper chamber postreoxygenation

(3) Targeting NLRP3 Inflammasome Alleviates Synovitis by Reducing Pyroptosis in Rats with Experimental Temporomandibular Joint OsteoarthritisMediators of InflammationNovember 23, 2022Yinzi Xin, Wei Wang, Enyu Mao, Hefeng Yang, Song Liinto M0 macrophages in 48 h. 2.5. Coculture of Macrophages and FLS Cells Transwell 6-well plate (3452; Corning, NY, USA) was used for coculture cells. FLS cells with density of 1 × 105 cells/ml were cultured

(4) Effect of HMGB1 on monocyte immune function in respiratory syncytial virus bronchiolitisExperimental and therapeutic medicineJanuary 1, 2021Niu M, Jiang Z, Xin X, Zhu J, Yang J, Diao M, Qi G, Qi B.until the cells grew to the appropriate concentration. Co-culture of macrophages Transwell plate (3452; Corning) was used for co-culture of monocytes and 16HBE. THP-1 monocytes with a density of 1x106/ml

(5) Biomolecular condensates undergo a generic shear-mediated liquid-to-solid transitionNature NanotechnologyOctober 1, 2020Yi Shen, Francesco Simone Ruggeri, Daniele Vigolo, Ayaka Kamada, Seema Qamar, Aviad Levin, Christiane Iserman, Simon Alberti, Peter St St George-Hyslop, Tuomas P. J. KnowlesGST-3C Protease. The protein aggregates were removed by centrifugation in Falcon Tubes for 4 min at 3452 x g. The protein was concentrated using Amicon Ultra-15-30k centrifugal filters at 3452 x g. The proteins

(6) Condensation of Ded1p Promotes a Translational Switch from Housekeeping to Stress Protein ProductionCellMay 14, 2020Iserman C, Desroches Altamirano C, Jegers C, Friedrich U, Zarin T, Fritsch AW, Mittasch M, Domingues A, Hersemann L, Jahnel M, et al.size exclusion chromatography (SEC), protein aggregates were removed by centrifugation in Falcon Tubes for 4 min at 3452 x g. If necessary, the protein was concentrated using Amicon Ultra-15-30k centrifugal filters at 3452

(7) Diapedesis-Induced Integrin Signaling via LFA-1 Facilitates Tissue Immunity by Inducing Intrinsic Complement C3 Expression in Immune CellsImmunityMarch 17, 2020Kolev M, West EE, Kunz N, Chauss D, Moseman EA, Rahman J, Freiwald T, Balmer ML, Lötscher J, Dimeloe S, et al.antibodies were from Biolegend unless otherwise indicated. T Cell Transmigration Six-well pates (3452, Costar, Corning) with 3 μM pore size inserts were coated with 1 ml of collagen type I (08–115, Millipore Sigma

(8) Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic ReprogrammingCell reportsDecember 11, 2018Simula L, Pacella I, Colamatteo A, Procaccini C, Cancila V, Bordi M, Tregnago C, Corrado M, Pigazzi M, Barnaba V, et al.Dickinson,http://www.bdbiosciences.com/in/instruments/lsr/index.jsp 3 μm-pore size transwell filters 24well,Corning Costar,Cat #3452 8 μm-pore size transwell filters 24well,Corning Costar,Cat #3464 Zeiss Axioscope A1,Zeiss,https://www.zeiss.com/microscopy/int/products/light-microscopes/axio-scope-a1-for-biology.html Volocity

(9) MiR-126 in intestinal-type sinonasal adenocarcinomas: exosomal transfer of MiR-126 promotes anti-tumour responsesBMC cancerSeptember 17, 2018Tomasetti M, Re M, Monaco F, Gaetani S, Rubini C, Bertini A, Pasquini E, Bersaglieri C, Bracci M, Staffolani S, et al.w/v), and counted using a stereomicroscope. Triple co-culture model A 3-μm trans-well insert (Costar 3452; Corning,) was first plated with 105 cultured IMR90 cells in an inverted position. After 6 h of incubation,

(10) A study on the biological function of heat shock factor 1 proteins in breast cancerOncology LettersJuly 2, 2018Xiaolan Wang, Dianlong Zhang, Mingqian Cao, Jinling Ba, Bin Wu, Tao Liu, Chen Nie24 mm Transwell® with 3 µm Pore was purchased from Corning Incorporated, Corning, NY, USA (cat. no. 3452); HSF1 lentiviruses and no-load control viruses were packaged by Shandong Vigene Biosciences Co., Ltd.

(11) A bioinspired microfluidic model of liquid plug-induced mechanical airway injuryBiomicrofluidicsJuly 1, 2018Song JW, Paek J, Park KT, Seo J, Huh D.lm pores (Costar 3450) for the cell culture region and polyester membranes with 3 lm pores (Costar 3452) for the plug generator. Microfluidic cell culture To form the airway epithelium in our model, we used

(12) A study on the biological function of heat shock factor 1 proteins in breast cancerOncology LettersJune 28, 2018Xiaolan Wang, Dianlong Zhang, Mingqian Cao, Jinling Ba, Bin Wu, Tao Liu, Chen Nie24 mm Transwell® with 3 µm Pore was purchased from Corning Incorporated, Corning, NY, USA (cat no: 3452); HSF1 lentiviruses and no-load control viruses were packaged by Shandong Vigene Biosciences Co., Ltd.

(13) Identification of glycerol-3-phosphate dehydrogenase 1 as a tumour suppressor in human breast cancerOncotargetNovember 24, 2017Cefan Zhou et al.Briefly, for cell migration assays, 1 × 105 cells were seeded onto the upper transwell chambers (Cat# 3452, Costar Corning, USA). and then they were all incubated for 24 h at 37°C. The lower chamber was added

(14) GDC-0449 improves the antitumor activity of nano-doxorubicin in pancreatic cancer in a fibroblast-enriched microenvironmentScientific reportsOctober 17, 2017Zhou Q, Zhou Y, Liu X, Shen Y.determined by the intensity of the bioluminescent signal. Indirect co-culture: The transwell (Corning, Cat.3452) co-culture system was used to establish the indirect co-culture model. NIH-3T3 cells were seeded on

(15) Genomic instability induced in distant progeny of bystander cells depends on the connexins expressed in the irradiated cellsInternational Journal of Radiation BiologyOctober 3, 2017Sonia M. de Toledo, Manuela Buonanno, Andrew L. Harris, Edouard I. AzzamTranswell inserts containing 10 μm-thick polyester permeable microporous membranes with 1 μm pores (Costar #3452) as we have recently described in detail (Domoguaer, De Toledo and Azzam 2016). Briefly, the cells destined

(16) ROLE OF REDUCING CELL LEAKAGE IN CELL CULTURE USING LARGE PORE SIZE PERMEABLE MEMBRANEJurnal TeknosainsAugust 30, 2017Nur Kaliwantoro, Marsetyawan HNE Soesatyo, Indarto Indarto, Mohammad Juffrie, Rini Dharmastiti, Suprihatin SuprihatinUniversity, Indonesia. 24 mm transwell polyester permeable membrane with 3 µm were supplied by Corning (cat. 3452). Culture media M199 and FBS were supplied by Gibco. 10 cm petridishes were supplied by Iwaki. Parallel

(17) Directional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium MonolayersScientific reportsJuly 7, 2017Klingeborn M, Dismuke WM, Skiba NP, Kelly U, Stamer WD, Bowes Rickman C.pore sizes of 0.4, 1.0, or 3.0 µm. Inserts (0.4 and 3.0 µm were from Corning (Transwell™, #3450 and 3452) while 1.0 µm inserts were from Greiner Bio-One (ThinCert™, #657610). Cells were seeded at high density

(18) Spermine Treated-Adipose Tissue-Derived Multi-Lineage Progenitor Cells Improve Left Ventricular Dysfunction in a Swine Model of Chronic Myocardial InfarctionJournal of Stem Cell Research & TherapyJanuary 1, 2016Hanayuki Okura, Mitsuko Morita(2 × 105 cells/well, each) were seeded onto the lower chamber of a 6-well-type Transwell (Cat. No. 3452. Corning, NY) while human cardiomyocytes (2 ×105 cells/well) (purchased from PromoCell), and pre-cultured

(19) Effect of dengue virus infection on the permeability of vero cells lineAIP Conference ProceedingsJanuary 1, 2016Nur Kaliwantoro, Marsetyawan Hne Soesatyo, Indarto, Mohammad Juffrie, Rini DharmastitiVero cells were grown on the 24 mm transwells polyester permeable membrane with pore size 3 μm (Corning cat. 3452) using complete media contains M199 and 10% FBS. To reduce cells escape through the relatively large

(20) Repression of miR-126 and upregulation of adrenomedullin in the stromal endothelium by cancer-stromal cross talks confers angiogenesis of cervical cancerOncogeneJuly 10, 2014T-H Huang, T-Y Chureverse transcriptase–PCR with the cocultured cell types displayed in the parentheses. A 3-mm Transwell insert (Costar 3452; Corning, NY, USA) was ﬁrst plated with 105 primarily cultured NAF on an inverted position. After 6

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