Fig 1: PDGF-C promotes fibroblast proliferation and migration but has no effect on tumour cells.a, Single-cell RNA-seq data from 26 human breast cancers (11 ER+, 5 HER2+ and 10 TNBC, triple negative)21. Expression of PDGFRA is shown in cancer and stromal cells. Number of cells sequenced is indicated in brackets. PVL, perivascular-like. Plots were generated using the Broad Institute single-cell portal, https://singlecell.broadinstitute.org. b, Additional data associated with Fig. 5c. CellTiter-Glo analysis following vehicle or rPDGF-C treatment (100 ng mL-1) for mouse (upper, middle) and human (lower) fibroblasts. Representative data of two (10T1/2 and CAF-2) or three (IMR90) independent repeats, in multiple cell lines. c, Transwell migration assay. Following overnight culture in SFM, 5,000 cells were seeded into Transwell inserts (8.0 µm pore size, Corning 3422) in SFM. DMEM plus 2% FBS containing vehicle or rPDGF-C (100 ng mL-1) was added per well. Treatments were refreshed at 24 hours. After 48 hours, inserts were washed, fixed (4% paraformaldehyde) and stained with DAPI. Non-migrated cells were removed from the top of the insert with a cotton tip. 4-5 images were taken per insert. Data show number of migrated CAF and TSAE1 cells per field of view. Representative data of three independent repeats. d, Ingenuity pathway analysis showing the top 6 significantly (P < 0.05, right-tailed Fisher’s exact test) altered pathways following treatment with rPDGF-C of MRC5 fibroblasts. Dashed line indicates P value cut-off. b,c, Data are presented as mean values. Source data