Corning® Ultra-Low Attachment 75cm² U-Flask Canted Neck Cell Culture Flask with Vent Cap from Corning Life Sciences

Product Specs
ItemCorning® Ultra-Low Attachment 75cm² U-Flask Canted Neck Cell Culture Flask with Vent Cap
CompanyCorning Life Sciences
Price Pricing Info Supplier Page View Company Product Page
Catalog Number3814
Quantity4 / Pk
Brand/TrademarkCorning®
MaterialCap Material: High density polyethylene
VolumeRecommended Medium Volume: 15 - 22.5 mL
ColorCap Color: Orange
SterilitySterile
TypeFlask

Specifications/Features

Neck Style: Canted

Cap Style: Vented

Flask Shape: U-shape

Surface Treatment: Ultra-Low Attachment

Add to Compare List

Corning Life Sciences

836 North Street

Building 300 Suite 3401

Tewksbury, MA 01876

United States

Phone: (978) 442-2200
Phone: (800) 492-1110

Email: clscustserv@corning.com

Company Profile

Website: www.corning.com/lifesciences

(1) 2D and 3D multiplexed subcellular profiling of nuclear instability in human cancerbioRxivNovember 16, 2023Shannon Coy, Brian Cheng, Jong Suk Lee, Rumana Rashid, Lindsay Browning, Yilin Xu, Sankha S. Chakrabarty, Clarence Yapp, Sabrina Chan, Juliann B. Tefft, et al.non-adherently at 37°C and 5% CO2 in Corning® Ultra-Low Attachment 75cm² U-Flasks (Corning, Catalog #3814). Media consisted of NeuroCult™ NS-A Proliferation Kit (StemCell, Catalog #05751) supplemented with

(2) Main Manuscript for Cell migration simulator-based biomarkers for glioblastomabioRxivJuly 21, 2023Jay Hou, Mariah McMahon, Jann N. Sarkaria, Clark C. Chen, David J. Odde[35,51]. UCSD cells prepared for experiments were propagated in ultra-low adhesion flasks (Corning 3814) with NSC Media and were allowed to recover thawing for several days prior to imaging. For adherent

(3) AzidoTMT Enables Direct Enrichment and Highly Multiplexed Quantitation of Proteome-Wide Functional ResiduesJournal of Proteome ResearchJuly 7, 2023Taylur P. Ma, Anita Izrael-Tomasevic, Rana Mroue, Hanna Budayeva, Sushant Malhotra, Ryan Raisner, Marie Evangelista, Christopher M. Rose, Donald S. Kirkpatrick, Kebing YuReactive Probe Labeling HCC1171 cells were grown in 3D ultralow attachment (ULA) plates (Corning, #3814) under standard culture conditions (RPMI medium supplemented with 2% glutamine and 10% FBS) at 40K cells/mL.

(4) Mesenchymal tumor organoid models recapitulate rhabdomyosarcoma subtypesEMBO molecular medicineOctober 10, 2022Meister MT, Groot Koerkamp MJA, de Souza T, Breunis WB, Frazer-Mendelewska E, Brok M, DeMartino J, Manders F, Calandrini C, Kerstens HHD, et al.an ultra‐low attachment culture flask (Corning Ultra‐Low Attachment 75 cm2 U‐Flask, Corning, cat no. 3814) in BM1* but without BME supplementation. Establishment of spheres of sufficient size took between 7

(5) JAZF1-SUZ12 dysregulates PRC2 function and gene expression during cell differentiationCell reportsMay 31, 2022Tavares M, Khandelwal G, Muter J, Viiri K, Beltran M, Brosens JJ, Jenner RG.LIF-free media and seeded as a single cell suspension on ultra-low attachment T75 flasks (Corning, 3814) at 2 × 106 cells per flask. LIF-free media was changed every second day and EBs were harvested at days

(6) Dual G9A/EZH2 Inhibition Stimulates Antitumor Immune Response in Ovarian High-Grade Serous CarcinomaMolecular cancer therapeuticsApril 1, 2022Spiliopoulou P, Spear S, Mirza H, Garner I, McGarry L, Grundland-Freile F, Cheng Z, Ennis DP, Iyer N, McNamara S, et al.spheroids were captured on a 40 μm membrane and placed into T75 ultra-low attachment flasks (ULA, Corning, 3814) and cultured in advanced DMEM/F12 medium (Life Technologies, 12634010), supplemented with 10% autologous

(7) Cancer stem-like cells evade CD8(+)CD103(+) tumor-resident memory T (T(RM)) lymphocytes by initiating an epithelial-to-mesenchymal transition program in a human lung tumor modelJournal for immunotherapy of cancerApril 1, 2022Corgnac S, Damei I, Gros G, Caidi A, Terry S, Chouaib S, Deloger M, Mami-Chouaib F.sphere cultures, IGR-Heu cells were cultured in ultra-low attachment Corning cell culture flasks (cat#3814 or cat#3815) or 6-well plates (cat# 3471) at 105 cells/mL in serum-free DMEM/F12 medium, supplemented

(8) Soft surfaces promote astrocytic differentiation of mouse embryonic neural stem cells via dephosphorylation of MRLC in the absence of serumScientific ReportsDecember 1, 2021Hiroshi Oyama, Akihiro Nukuda, Seiichiro Ishihara, Hisashi Haganeural stem cell medium (KOHJIN BIO, Saitama, Japan) were cultured in ultra-low attachment culture flasks (3814; Corning). The culture medium was added twice daily. The cells were passaged into new flasks 3 days

(9) Paradoxical implication of BAX/BAK in the persistence of tetraploid cellsCell Death & DiseaseNovember 1, 2021Jiayin Deng et al.Netherlands). Antibodies Rabbit monoclonal antibody (Ab) against Bax (#2772) and rabbit polyclonal Ab against Bak (#3814) were purchased from Cell Signaling Technology (Danvers, MA, USA). Mouse monoclonal Ab against p27 Kip1

(10) IgG Immune Complexes Inhibit Naïve T Cell Proliferation and Suppress Effector Function in Cytotoxic T CellsFrontiers in ImmunologyAugust 10, 2021Wissam Charab et al.every 1mL of T cell suspension. Resuspended cells were placed in ultra-low-attachment flasks (Corning #3814) to minimize adhesion and cell loss. Flasks were then incubated for 20-30 minutes in a 37°C 5% CO2 incubator.

(11) Spheroid-Like Cultures for Expanding Angiopoietin Receptor-1 (aka. Tie2) Positive Cells from the Human Intervertebral DiscInternational journal of molecular sciencesDecember 10, 2020Zhang X, Guerrero J, Croft AS, Albers CE, Häckel S, Gantenbein B.with Phosphate-buffered saline (PBS). The ultra-low attachment surface flask was from Corning (#cat 3814 Wiesbaden, Germany), and ~200,000 NPCs were seeded into each flask. The culture media was low glucose

(12) DDX3X Suppresses the Susceptibility of Hindbrain Lineages to MedulloblastomaDevelopmental cellAugust 24, 2020Patmore DM, Jassim A, Nathan E, Gilbertson RJ, Tahan D, Hoffmann N, Tong Y, Smith KS, Kanneganti TD, Suzuki H, et al.2014)",www.bioconductor.org/packages/release/bioc/html/QDNAsea Other,Other,Other Ultra-low attachment T75 flask,Corning,Cat# 3814 KEY RESOURCES TABLE

(13) Drugs Targeting Tumor-Initiating Cells Prolong Survival in a Post-Surgery, Post-Chemotherapy Ovarian Cancer Relapse ModelCancers (Basel)June 21, 2020Brittney S. Harrington et al.grown adherently in 75 cm flasks in RPMI or in TIC-enriching culture conditions in 75 cm ULA flasks (3814 Corning). RNA quality was assessed on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA,

(14) Nilotinib, an approved leukemia drug, inhibits smoothened signaling in Hedgehog-dependent medulloblastomaPLOS ONESeptember 20, 2019Kirti Kandhwal Chahal, Jie Li, Irina Kufareva, Milind Parle, Donald L. Durden, Robert J. Wechsler-Reya, Clark C. Chen, Ruben Abagyanng/ml EGF + 1× AAS] or adherent culture media (DMEM+10% FBS + 1× AAS) in ultra-low attachment (Corning, 3814) or tissue culture treated (Corning, 430641U) flasks, respectively. The cells were plated at 1000 cells

(15) Sleeping beauty genetic screen identifies miR-23b::BTBD7 gene interaction as crucial for colorectal cancer metastasisEBioMedicineAugust 1, 2019Grisard E, Coan M, Cesaratto L, Rigo I, Zandonà L, Paulitti A, Andreuzzi E, Rampioni Vinciguerra GL, Poletto E, Del Ben F, et al.(T0 cells that never underwent fSCS), sequential rounds of fSCS were performed on ULA T75 flasks (#3814, Corning). Cells surviving from the first round of fSCS were pooled together and expanded to generate

(16) Evaluation of anticancer agents using patient-derived tumor organoids characteristically similar to source tissuesOncology ReportsJune 18, 2018Hirosumi Tamura et al.negative. Cell culture F-PDOs were cultured in 15 ml FBIM002 using ultra-low attachment 75-cm2 flasks (cat. no. 3814; Corning Incorporated) at 37°C in a humidified incubator with 5% CO2. Since accurate cell numbers of

(17) A High-Throughput Screening Model of the Tumor Microenvironment for Ovarian Cancer Cell GrowthSLAS DiscoveryJune 1, 2017Madhu Lal-Nag, Lauren McGee, Rajarshi Guha, Ernst Lengyel, Hilary A. Kenny, Marc FerrerMA). For cells forming spheres conditions, spheres were first grown in a T75 ULA flask (Corning, Cat#3814) in 50 ml of SCM+KO+ITS with 5000 cells per ml for 7–14 days. Spheres were then dispersed with Trypsin

(18) Large-scale pharmacological profiling of 3D tumor models of cancer cellsCell death & diseaseDecember 1, 2016Mathews Griner LA, Zhang X, Guha R, McKnight C, Goldlust IS, Lal-Nag M, Wilson K, Michael S, Titus S, Shinn P, et al.forming spheres, spheres were first grown in a T75 ULA flask (Corning, New York, NY, USA; catalog number 3814) in 50 ml of SCM media with 5000 cells per ml for 7–14 days. Spheres were then dispersed with Trypsin

(19) Drug sensitivity of single cancer cells is predicted by changes in mass accumulation rateNature biotechnologyNovember 1, 2016Stevens MM, Maire CL, Chou N, Murakami MA, Knoff DS, Kikuchi Y, Kimmerling RJ, Liu H, Haidar S, Calistri NL, et al.epidermal growth factor (EGF), 10 ng/ml human bFGF in ultra-low attachment coated flasks (Corning, Cat#3814), which were kept in a 37 °C, 5% CO2, and humidified incubator. All PDCL’s tested negative for mycoplasma. Prior

(20) Registered report: Wnt activity defines colon cancer stem cells and is regulated by the microenvironmenteLifeAugust 19, 2015James Evans, Anthony Essex, Hong Xin, Nurith Amitai, Lindsey Brinton, Erin Griner, Reproducibility Project: Cancer Biology, Reproducibility Project Cancer Biologyflask,Labware,Corning,3815,Original catalog # not specified 75 cm2 ultralow- attachment tissue culture flask,Labware,Corning,3814,Original catalog # not specified *From (Todaro et al., 2007).

Be the First to Write a Review!