Mini-PROTEAN Tetra Cell for Ready Gel Precast Gels from Bio-Rad

Product Specs
ItemMini-PROTEAN Tetra Cell for Ready Gel Precast Gels
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog Number1658004
Quantity1EA
Brand/TrademarkBio-Rad Laboratories, Life Science Group
Dimensions1 to 4 mini gels
Type8.6 × 6.7 cm (Mini-PROTEAN precast gels) or handcast gels

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Dopaminergic Input Regulates the Sensitivity of Indirect Pathway Striatal Spiny Neurons to Brain-Derived Neurotrophic FactorBiology (Basel)October 23, 2023Maurilyn Ayon-Olivas, Daniel Wolf, Thomas Andreska, Noelia Granado, Patrick Lüningschrör, Chi Wang Ip, Rosario Moratalla, Michael Sendtnerand Western Blotting Polyacrylamide gel electrophoresis was performed in an electrophoresis cell chamber (Bio-Rad, Hercules, CA, USA #1658004) at 70 V for the stacking gel; then, the voltage was changed to 120 V once the samples reached the separating

(2) Systemic gene therapy using an AAV44.9 vector rescues a neonatal lethal mouse model of propionic acidemiaMolecular Therapy - Methods & Clinical DevelopmentSeptember 14, 2023Randy J. Chandler, Giovanni Di Pasquale, Eun-Young Choi, David Chang, Stephanie N. Smith, Jennifer L. Sloan, Victoria Hoffmann, Lina Li, John A. Chiorini, Charles P. Vendittiusing the Mini-PROTEAN Tetra Vertical Electrophoresis Cell with Mini-PROTEAN TGX Gels (catalog numbers 1658004 and 4561084, Bio-Rad, respectively). Protein transfer was accomplished using a Trans-Blot Turbo Transfer

(3) Creating Optimal Conditions for OPA1 Isoforms by Western Blot in Skeletal Muscle Cells and TissuebioRxivAugust 19, 2023Dominique C. Stephens, Margaret Mungai, Amber Crabtree, Heather K. Beasley, Edgar Garza-Lopez, Kit Neikirk, Serif Bacevac, Larry Vang, Zer Vue, Neng Vue, et al.needle,Amazon,LY-999 Tris-glycine Mini Gels,Novex,XP00100BOX Vertical Electrophoresis System,Bio-Rad,1658004 Nitrocellulose membrane,Bio-Rad,1620115 Microcentrifuge,Eppendorf Digital Centrifuge,5415D Key

(4) Progranulin Protects against Hyperglycemia-Induced Neuronal Dysfunction through GSK3β SignalingCellsJuly 7, 2023Dedert C, Salih L, Xu F.0.45 µm nitrocellulose membranes (Bio-Rad, Cat# 1620115) in a Mini Protean Tetra System (Bio-Rad, Cat# 1658004). Membranes were blocked with 5% milk in TBST, then blotted using primary antibodies for p-Akt ser473

(5) A conditional strategy for cell-type-specific labeling of endogenous excitatory synapses in DrosophilaCell Reports MethodsMay 22, 2023Michael J. Parisi, Michael A. Aimino, Timothy J. Moscablue, 5% β-mercaptoethanol) to be run on SDS-PAGE gels using the Mini Protean system (Bio-Rad, no. 1658004). Samples were heated at 95°C for 5 min and loaded on 4–15% TGX gels (Bio-Rad, no. 4568083) in running

(6) Expression and Purification of scFv2H7-P18F3, a Bi-Modular Fusion Protein (BMFP) Targeting Human CD20BIO-PROTOCOLMay 20, 2023Carine Brousse, Nathan E. Rainey, Alexandra Desrames, Jean-Luc Teillaud, Benoît Gamain, Arnaud Chêne612-3839; 612-3840)Vertical gel electrophoresis system (PROTEAN Tetra cell) (Bio-Rad, catalog number: 1658004)Autoclave (to be run at 120 °C for 20 min, under saturated steam ≥ 15 psi)Water bath allowing precise

(7) Functionally distinct promoter classes initiate transcription via different mechanisms reflected in focused versus dispersed initiation patternsThe EMBO JournalMay 15, 2023Leonid Serebreni, Lisa‐Marie Pleyer, Vanja Haberle, Oliver Hendy, Anna Vlasova, Vincent Loubiere, Filip Nemčko, Katharina Bergauer, Elisabeth Roitinger, Karl Mechtler, et al.2014",https://bioconductor.org/packages/release/bioc/html/DESeq2.html Other,Other,Other Mini‐PROTEAN Tetra Vertical Electrophoresis Cell,Bio‐Rad,Cat#1658004 Monarch Gel Extraction,NEB,Cat#T1020L Illumina Truseq small RNA library prep kit,Illumina,Cat#RS‐200‐0012 Power Blotter Station

(8) Evidence that RXFP4 is located in enterochromaffin cells and can regulate production and release of serotoninBioscience ReportsApril 26, 2023Shalinda J.A. Fernando, Qian Wang, Debbie L. Hay, Ross A.D. Bathgate, Peter R. Shepherd, Kate L. Leewas loaded at 20–40 µg and electrophoresis was performed in Mini-PROTEAN tetra Cell System (Bio-Rad # 1658004) at 80 V for 30 min after which the voltage was increased to 120 V until the dye front ran off the bottom

(9) Age-dependent Pdgfrβ signaling drives adipocyte progenitor dysfunction to alter the beige adipogenic niche in male miceNature communicationsMarch 31, 2023Benvie AM, Lee D, Steiner BM, Xue S, Jiang Y, Berry DC.(Biorad 4561034). The gel was placed into a Mini-PROTEAN Tetra Electrophoresis Cell chamber (Biorad 1658004) suspended in 1x running buffer (Biorad 1610744) and ran at 90 V for ~2.5 h. Protein was then transferred

(10) Executing cell-specific cross-linking immunoprecipitation and sequencing (seCLIP) in C. elegansSTAR ProtocolsMarch 1, 2023Stephen M. Blazie, Yishi JinScientific,Cat#09-528-110B Mini-PROTEAN® Tetra Vertical Electrophoresis Cell for Mini Precast Gels (for SDS PAGE),Bio-Rad,Cat#1658004 Nitrocellulose membrane (0.45 μM),Bio-Rad,Cat#1620115 Phase Lock Gel Heavy,VWR,Cat#10847-802 Plastic

(11) Protocol for absolute quantification of proteins in Gram-negative bacteria based on QconCAT-based labeled peptidesSTAR ProtocolsMarch 1, 2023Nicolás Gurdo, Shannara Kayleigh Taylor Parkins, Martina Fricano, Tune Wulff, Lars Keld Nielsen, Pablo Iván Nikelresolving gel,Bio-Rad,Cat#4561096 Mini-PROTEAN tetra cell vertical gel electrophoresis system,Bio-Rad,Cat#1658004 Spatula or Knife Gel,Thermo Fisher,Cat#EI9010

(12) Evaluation of Caspase Activation to Assess Innate Immune Cell DeathJournal of visualized experiments : JoVEJanuary 20, 2023Han JH, Tweedell RE, Kanneganti TD.Scientific,221S, DMEM,Thermo Fisher Scientific,11995–073, DTT,Sigma,43815, Eelectrophoresis apparatus,Bio-Rad,1658004, Ethanol,Pharmco,111000200, Fetal bovine serum,Biowest,S1620, Filter paper,Bio-Rad,1703965, Forceps,Fisher

(13) Extensive proteome and functional genomic profiling of variability between genetically identical human B-lymphoblastoid cellsScientific DataDecember 10, 2022Miklós Laczik, Edina Erdős, Lilla Ozgyin, Zsuzsanna Hevessy, Éva Csősz, Gergő Kalló, Tibor Nagy, Endre Barta, Szilárd Póliska, István Szatmári, et al.sample were loaded to 12% polyacrilamide gel and separated in a Bio-Rad mini tetra cell (Bio-Rad, cat. 1658004) on 100 V constant voltage for 1.5 hours. The protein bands were stained using PageBlue protein staining solution

(14) Protocol to detect nucleotide-protein interaction in vitro using a non-radioactive competitive electrophoretic mobility shift assaySTAR ProtocolsDecember 1, 2022Fang Wang, Ting Yao, Wen Yang, Pan Wu, Yutao Liu, Bin YangExtra Thick Blot Paper,Bio-Rad,Cat# 1703955 Oven,Tianyu,DGG-10 Water Bath,OuNuo,SY-1-2 Mini-PROTEAN Tetra cell,Bio-Rad,1658004 Mini Trans-Blot module,Bio-Rad,1658036 Image system,GE,Amersham Imager 600 0.2 mL PCR tube,Axygen,PCR-02D-C 0.22

(15) Native protein purification of ferroxidase LPR1 from leaf extracts of a transgenic Arabidopsis thaliana lineSTAR ProtocolsDecember 1, 2022Nancy Tang, Christin NaumannRoth,XA96.1 Centrifuge,Eppendorf,5804R AKTA Purifier 10,GE Healthcare,N/A Mini-PROTEAN Tetra Vertical Electrophoresis Cell,Bio-Rad,# 1658004 Power Blotter XL System,Thermo Scientific,PB0013 HighLoad Superdex 16/60 200 pg,GE Healthcare,GE28-9893-35 "Superloop,

(16) A protocol to characterize zebrafish LGP2 as a dual regulator of IFN response during viral infectionSTAR ProtocNovember 11, 2022Xiu-Ying Gong, Yi-Bing Zhang8.0,Dotmatics,https://www.graphpad.com/ ,, Other,Other,Other ,, Mini-PROTEAN® tetra system,BIO-RAD,Cat# 1658004 Mini Trans-Blot® Electrophoretic Transfer Cell,BIO-RAD,Cat# 170-3930 Mini-PROTEAN® system casting

(17) Two neuronal peptides encoded from a single transcript regulate mitochondrial complex III in DrosophilaeLifeNovember 8, 2022Justin A Bosch, Berrak Ugur, Israel Pichardo-Casas, Jordan Rabasco, Felipe Escobedo, Zhongyuan Zuo, Ben Brown, Susan Celniker, David A Sinclair, Hugo J Bellen, et al.1610744). Gels were ran at 100–200 V in a Mini-PROTEAN Tetra Vertical Electrophoresis Cell (Biorad 1658004). Proteins were transferred to Immobilon-FL PVDF (Millipore IPFL00010) in transfer buffer (25 mM Tris,

(18) Generation and Characterization of Native and Sialic Acid-Deficient IgEInt J Mol SciNovember 3, 2022Alex J. McCraw, Richard A. Gardner, Anna M. Davies, Daniel I. R. Spencer, Melanie Grandits, Gerd K. Wagner, James M. McDonnell, Sophia N. Karagiannis, Alicia Chenoweth, Silvia Crescioliwere run at 150 V for 45 mins on a Mini-PROTEAN Tetra Vertical Electrophoresis Cell [Bio-Rad, Cat. #1658004] and visualized using InstantBlue Protein Stain [Sigma-Aldrich, St. Louis, MO, USA, Cat. ISB1L]. 4.6.

(19) Activity-Induced Cortical Glutamatergic Neuron Nascent ProteinsThe Journal of neuroscience : the official journal of the Society for NeuroscienceOctober 19, 2022Schiapparelli LM, Xie Y, Sharma P, McClatchy DB, Ma Y, Yates JR 3rd, Maximov A, Cline HT.β-mercaptoethanol. Inputs and NeutrAvidin purified protein were run in a Mini-PROTEAN electrophoresis cell (catalog #1658004, BIO-RAD; and compatible precast gels) and transferred to nitrocellulose membranes using a Trans-Blot Turbo Transfer System

(20) Developmental and housekeeping transcriptional programs in Drosophila require distinct chromatin remodelersMolecular CellOctober 1, 2022Oliver Hendy, Leonid Serebreni, Katharina Bergauer, Felix Muerdter, Lukas Huber, Filip Nemčko, Alexander Stark"4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, 15-well, 15 μl",Bio-Rad,#34561096 Mini-PROTEAN Tetra Vertical Electrophoresis Cell,Bio-Rad,1658004 Monarch Gel Extraction,NEB,T1020L Deposited data,Deposited data,Deposited data Raw and analyzed sequencing

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5/27/2021A Reliable Vertical Electrophoresis Unit sndp kmrwestern blotting is a gold standard technique for studying protein expression in biological samples. the results of the western blotting depend on the proper separation of the protein samples. bio rad... Read Review
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4/18/2018Great Chambers For Electrophoresis Rebecca WatesWe have identified a class of ATPase inhibitors that show promise for treatment of ovarian cancer. We are currently evaluating what mechanisms, if any, may lead to resistance to theses ATPase ... Read Review