BioPak® application pak from MilliporeSigma

Product Specs
ItemBioPak® application pak
CompanyMilliporeSigma
Price Inquire
Catalog NumberCDUFBI001
ApplicationsVirus Characterization: RT-PCR Analysis

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MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

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Website: www.SigmaAldrich.com/

(1) RAFT Polymer–Antibody Conjugation: Squaramide Ester Chemistry Leads to Conjugates with a Therapeutic Anti-EGFR Antibody with Full Retention of Activity and Increased Tumor Uptake In VivoMolecular PharmaceuticsJune 5, 2023Aditya Ardana, Saikat Ghosh, Pie Huda, Nicholas L. Fletcher, Kristofer J. Thurecht, Charlotte C. Williamspurchased from Merck. The water used was of ultrapure grade (purified by a Milli-Q purification system, Biopak Polisher). Anti-EGFR mouse 528mAb was provided by the fermentation team at the Recombinant Protein Production

(2) Continuous Square Wave Voltammetry for High Information Content Interrogation of Conformation Switching SensorsACS Measurement Science AuFebruary 15, 2023Sanduni W. Abeykoon, Ryan J. Whiteand were used as received. Solutions were prepared with ultrapure water (18.0 MΩ cm at 25 °C) using a Biopak Polisher Millipore ultrapurification system (Millipore, Billerica, MA). Parent tobramycin sequence: 5′-HS-C6-GGGACTTGGTTTAGGT-AATGAGTCCC-MB-3′;14

(3) Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA AptamersJournal of Visualized ExperimentsAugust 29, 2022Madeline M. Mumbleau, Madeline R. Meyer, Ming C. Hammondtips,Sigma-Aldrich,"Z369004, AXYT200CR, AXYT1000CR", Millipore water filter with BioPak unit,Sigma-Aldrich,"CDUFBI001, ZRQSVR3WW", Narrow micropipettor pipette tips,DOT Scientific,RN005R-LRS, "PBS, 10x",Thermo Fischer

(4) An adaptable microreactor to investigate the influence of interfaces on Pseudomonas aeruginosa biofilm growthApplied Microbiology and BiotechnologyFebruary 1, 2022Zhang Ye, Dina M. Silva, Daniela Traini, Paul Young, Shaokoon Cheng, Hui Xin Ongwas supplied by MP (Biomedical Australasia Pty Limited, Australia). Milli-Q water was obtained from Biopak® Polisher system (Merck KGaA, Germany). Device design and fabrication The microreactor (Fig. 1) consists of

(5) 3-D printed microfluidics for rapid prototyping and testing of electrochemical, aptamer-based sensor devices under flow conditionsAnalytica Chimica ActaFebruary 1, 2022Israel Belmonte, Ryan J. WhiteLake Bluff, IL, USA) All solutions were prepared using ultrapure water (18.0 MΩ cm at 25°C) using a Biopak Polisher Millipore ultrapurification system (Millipore, Billerica, MA). The ATP aptamer (5’-HS-C6-CTGGGGGAGTATTGCGGAGGAAA-MB-3’)

(6) SH3BP4 promotes neuropilin-1 and α5-integrin endocytosis and is inhibited by AktDevelopmental cellApril 19, 2021Burckhardt CJ, Minna JD, Danuser G.columns (Sydlab, Natick, MA, MB082) and eluted in ultrapure water (Millipore Synergy UV, Biopak filter CDUFBI001). The pEGFP-N1 vector (Clontech, Mountain View, CA, 6085-1) was first modified at position alanine-206

(7) Nonwoven Ion-Exchange Membranes with High Protein Binding Capacity for BioseparationsMembranes (Basel)March 6, 2021Solomon Mengistu Lemma, Cristiana Boi, Ruben G. CarbonellUniversity. Buffers were prepared from analytical-grade chemicals and deionized water (Milli-Q system fitted with BioPak® polisher, MilliporeSigma, Bedford, MA, USA). 20 mM Sodium acetate buffer, pH 5.5 (for cation exchange membranes),

(8) Deep sequencing of non-enzymatic RNA primer extensionNucleic Acids ResearchJuly 9, 2020Daniel Duzdevich, Christopher E Carr, Jack W Szostakheat-distributive aluminum or iron face-plates, in TBE prepared in Milli-Q water (Milli-Q Reference with Biopak Polisher, Millipore Sigma). Gels requiring staining (in particular, gels used to assay the sequencing constructs,

(9) Determination of antibiotic residues in frozen poultry by a solid-phase dispersion method using liquid chromatography-triple quadrupole mass spectrometryToxicology ReportsSeptember 17, 2019Abdulrasaq O. Oyedeji, Titus A.M. Msagati, Akan B. Williams, Nsikak U. Benson(Ultrapure water) used in this study was produced using an Integral 10 Elix Milli-Q system with an LC (Biopak) polisher (Massachusetts, USA). Other apparatuses used include the Waring laboratory blender – Z272205 (Sigma-Aldrich,

(10) Direct, Real-Time Detection of Adenosine Triphosphate Release from Astrocytes in Three-Dimensional Culture Using an Integrated Electrochemical Aptamer-Based SensorACS Chemical NeuroscienceApril 17, 2019Mirelis Santos-Cancel, Laura W. Simpson, Jennie B. Leach, Ryan J. WhiteSigma- Aldrich. All solutions were prepared using autoclaved, ultrapure water (18.0 MΩ cm at 25°C) using a Biopak Polisher Millipore ultra purification system (Millipore, Billerica, MA). Rat primary cortical astrocytes and

(11) Electrochemical Aptamer-Based Sensor for Real-Time Monitoring of InsulinACS SensorsFebruary 22, 2019Yao Wu, Beksultan Midinov, Ryan J. Whiteanalytical grade. All of the solutions were prepared using ultrapure deionized (DI) water purified with a Biopak Polisher (18.2 MΩ•cm, Millipore, Billerica, MA). The interrogation buffer was Tris buffer (50 mM tris, 10 mM

(12) In vitro Generation of CRISPR-Cas9 Complexes with Covalently Bound Repair Templates for Genome Editing in Mammalian CellsBIO-PROTOCOLJanuary 5, 2019Nataša Savić, Femke Cas Ringnalda, Christian Berk, Katja Bargsten, Jonathan Hall, Martin Jinek, Gerald Schwankpurification system (e.g., EMD Millipore Synergy UV equipped with a Biopak polisher [Merck, catalog number: CDUFBI001]) Semi-preparative high-performance liquid chromatography (HPLC) system equipped with an autosampler,

(13) A Flowthrough Assay for Rapid Bedside Stratification of Bloodstream Bacterial Infection in Critically Ill Patients: a Pilot StudyJournal of Clinical MicrobiologySeptember 1, 2018Pramod Jagtap, Rohini Singh, Karuna Deepika, Venkataraman Sritharan, Shalini Guptagrade. All solutions were prepared using ultrapure deionized Milli-Q water (⬃18.8 m⍀ · cm resistivity, CDUFBI001; Millipore, USA). Due to the known pyrogenicity and toxicity of LPS and LTA, strict safety guidelines

(14) Rapid Two-Millisecond Interrogation of Electrochemical, Aptamer-Based Sensor Response Using Intermittent Pulse AmperometryACS SensorsJune 22, 2018Mirelis Santos-Cancel, Robert A. Lazenby, Ryan J. WhiteAldrich. All solutions were prepared using autoclaved, ultrapure water (18.0 MΩ cm at 25 °C) using a Biopak Polisher Millipore ultra-purification system (Millipore, Billerica, MA). The DNA tobramycin aptamer sequence

(15) Dual functionality nanobioconjugates targeting intracellular bacteria in cancer cells with enhanced antimicrobial activityScientific ReportsDecember 1, 2017Rohini Singh, Smita Patil, Neetu Singh, Shalini GuptaAll solutions were prepared using ultrapure deionised (DI) Milli-Q water (∼18.8 mΩ.cm resistivity) (CDUFBI001, Millipore, USA). HeLa cell line was obtained from the National Centre for Cell Science (NCCS) (Pune,

(16) FISH-Flow, a protocol for the concurrent detection of mRNA and protein in single cells using fluorescence in situ hybridization and flow cytometryNature protocolsJune 1, 2017Arrigucci R, Bushkin Y, Radford F, Lakehal K, Vir P, Pine R, Martin D, Sugarman J, Zhao Y, Yap GS, et al.water purification system) Biopak filter for Milli-Q purification system (EMD Millipore, cat. no. CDUFBI001) Nuclease-free water (Thermo Fisher Scientific, cat. no. AM9939) 10× PBS (RNase-free; Thermo Fisher Scientific

(17) Collagen Membranes with Ribonuclease Inhibitors for Long-Term Stability of Electrochemical Aptamer-Based Sensors Employing RNAAnalytical ChemistryMay 16, 2017Mirelis Santos-Cancel, Ryan J. WhiteSciences. All solutions were prepared using autoclaved, ultrapure water (18.0 MΩ cm at 25 °C) using a Biopak Polisher Millipore ultra-purification system (Millipore, Billerica, MA). The RNA aminoglycoside aptamer sequence

(18) Quantitative Microplate Assay for Real-Time Nuclease KineticsPLOS ONEApril 21, 2016Jonas Eriksson, Ülo Langelproduced by purifying ddH2O using a Milli-Q Advantage A10 Water Purification System equipped with a BioPak Polisher (Merck Millipore). The purified water had undetectable levels of Pi. Tris amino Ultra-pure (Angus Chemical

(19) A High-Throughput Kinetic Assay for RNA-Cleaving DeoxyribozymesPLOS ONEAugust 26, 2015Jonas Eriksson, Henrik Helmfors, Ülo Langelultrapure water was produced using a Milli-Q Advantage A10 Water Purification System equipped with a BioPak® Polisher from Merck Millipore. Phusion High-Fidelity PCR kit and TranscriptAid T7 High Yield Transcription kit

(20) Viral Quantitative Capillary Electrophoresis for Counting and Quality Control of RNA VirusesAnalytical ChemistryNovember 6, 2012Afnan Azizi, Gleb G. Mironov, Darija Muharemagic, Mohamed Wehbe, John C. Bell, Maxim V. Berezovskisystem (cat. no. SYNSV00WW) supplied with a 13 kDa cut-off, BioPak Point-of-Use ultrafilter (cat. no. CDUFBI001, Millipore, MA, USA). Preparation of rRNA and DNA standards, virus lysis, RNase A and NaOH treatments:

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BioPak® application pak from MilliporeSigma

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BioPak® application pak from MilliporeSigma

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