SphI-HF from New England Biolabs

Product Specs
ItemSphI-HF
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR3182S
Concentration20,000 units/ml
Quantity500 units
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

GCATG^C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Unbiased assessment of genome integrity and purging of adverse outcomes at the target locus upon editing of CD4+ T‐cells for the treatment of Hyper IgM1The EMBO JournalDecember 1, 2023Daniele Canarutto, Claudia Asperti, Valentina Vavassori, Simona Porcellini, Elisabetta Rovelli, Marianna Paulis, Samuele Ferrari, Angelica Varesi, Martina Fiumara, Aurelien Jacob, et al.estimate the frequency of concatemers, samples were digested or not with SphI‐HF in rCutsmart buffer (R3182S, New England Biolabs) for 1 h at 37°C and then heat inactivated for 20 min at 80°C. ddPCR assay was

(2) Executing cell-specific cross-linking immunoprecipitation and sequencing (seCLIP) in C. elegansSTAR ProtocolsMarch 1, 2023Stephen M. Blazie, Yishi Jin(NaCl),Fisher Scientific Sigma-Aldrich,Cat#PRN2615 S9888 SpeI-HF®,NEB,Cat#R3133S SphI-HF®,NEB,Cat#R3182S SuperSignal™ West Pico PLUS Chemiluminescent Substrate,Thermo Fisher,Cat#34579 T4 polynucleotide kinase,NEB,Cat#M0201S T4 polynucleotide kinase (10 U/μL

(3) Regulation of hierarchical carbon substrate utilization, nitrogen fixation, and root colonization by the Hfq/Crc/CrcZY genes in Pseudomonas stutzeriiScienceDecember 22, 2022Fanyang Lv, Yuhua Zhan, Wei Lu, Xiubin Ke, Yahui Shao, Yiyuan Ma, Juan Zheng, Zhimin Yang, Shanshan Jiang, Liguo Shang, et al.,Thermo Scientific,Cat# R0861 RNAlater solution,Invitrogen,Cat# AM7020 Xba I,NEB,Cat# R0145S Sph I-HF,NEB,Cat# R3182S Nhe I-HF,NEB,Cat# R3131S EcoR I-HF,NEB,Cat# R3101S BamH I -HF,NEB,Cat# R3136S Hind III-HF,NEB,Cat#

(4) Adaptive sequence divergence forged new neurodevelopmental enhancers in humansCellNovember 23, 2022Riley J. Mangan, Fernando C. Alsina, Federica Mosti, Jesús Emiliano Sotelo-Fonseca, Daniel A. Snellings, Eric H. Au, Juliana Carvalho, Laya Sathyan, Graham D. Johnson, Timothy E. Reddy, et al.Scientific,Epredia 6502 Phusion DNA Polymerase,New England Biolabs,Cat# M0530S SphI,New England Biolabs,Cat# R3182S Trypsin-EDTA,Sigma-Aldrich,Cat# 59428C Vectashield,Vector Laboratories,H-1000-10 ZymoPURE II Plasmid

(5) Osteoclast-mediated resorption primes the skeleton for successful integration during axolotl limb regenerationeLifeOctober 11, 2022Camilo Riquelme-GuzmÃ¡n, Stephanie L Tsai, Karen Carreon Paz, Congtin Nguyen, David Oriola, Maritta Schuez, Jan BruguÃ©s, Joshua D Currie, Tatiana Sandoval-GuzmÃ¡nusing the restriction enzyme SpeI-HF (#R3133S, New England BioLabs) for Ctsk and Krt17, or SphI-HF (#R3182S, New England BioLabs) for Kazald1. Probes were purified using the RNAeasy Mini Kit according to provider’s

(6) An optimized FusX assembly-based technique to introduce mitochondrial TC-to-TT variations in human cell linesSTAR ProtocolsJune 17, 2022Bibekananda Kar, Ankit Sabharwal, Santiago Restrepo-Castillo, Brandon W. Simone, Karl J. Clark, Stephen C. Ekker,New England BioLabs,Cat#R0739S Esp3I,Thermo Fisher Scientific,Cat#ER0451 SphI,New England BioLabs,Cat#R3182S XbaI,New England BioLabs,Cat#R0145S ATP,Thermo Fisher Scientific,Cat#R0441 T4 DNA Ligase Buffer,New England BioLabs

(7) A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeastBMC GenomicsDecember 1, 2021Nicholas J. McGlincy, Zuriah A. Meacham, Kendra K. Reynaud, Ryan Muller, Rachel Baum, Nicholas T. Ingoliaeluted DNA from the previous digestion in 1x final concentration CutSmart buffer with 20 U SphI-HF (NEB R3182S). Digestion was carried out for 1 h at 37 °C, and DNA was purified again using a Zymo DNA Clean & Concentrator

(8) Development and optimization of an in vivo electrocardiogram recording method and analysis program for adult zebrafishDisease Models & MechanismsAugust 1, 2021ThuyVy Duong et al.around a T>G point mutation. After PCR, a 30 µl reaction was set up using the restriction enzyme sphI (R3182S; New England Biolabs). This reaction was incubated at 37°C for 1 h, followed by 20 min of heat inactivation

(9) Increased expression of PolÎ´ does not alter the canonical replication program in vivoWellcome Open ResearchMay 4, 2021RÃ³bert Zach, Antony M. Carrwere performed according to manufacturer’s instructions with the following restriction enzymes: SphI-HF (NEB, R3182S); ApaI (NEB, R0114S); XhoI (NEB, R0146S); SacI-HF (NEB, R3156S); SpeI-HF (NEB, R3133S); SbfI-HF (NEB

(10) Scalable characterization of the PAM requirements of CRISPR–Cas enzymes using HT-PAMDANature ProtocolsMarch 1, 2021Russell T. Walton, Jonathan Y. Hsu, J. Keith Joung, Benjamin P. KleinstiverM0212S) EcoRI-HF (New England BioLabs, cat. no. R3101S) SphI-HF (New England BioLabs, cat. no. R3182S) SpeI-HF (New England BioLabs, cat. no. R3133S) PvuI-HF (New England BioLabs, cat. no. R3150S)

(11) Increased expression of Polδ does not alter the canonical replication program in vivoWellcome Open ResearchFebruary 26, 2021Róbert Zach, Antony M. Carrwere performed according to manufacturer’s instructions with the following restriction enzymes: SphI-HF (NEB, R3182S); ApaI (NEB, R0114S); XhoI (NEB, R0146S); SacI-HF (NEB, R3156S); SpeI-HF (NEB, R3133S); SbfI-HF (NEB,

(12) Rapid and Efficient Synthetic Assembly of Multiplex Luciferase Reporter Plasmids for the Simultaneous Monitoring of Up to Six Cellular Signaling PathwaysCurrent Protocols in Molecular BiologyJune 26, 2020Alejandro Sarrion‐Perdigones, Yezabel Gonzalez, Koen J.T. Venkenno. R3195S), KpnI-HF (New England Biolabs, cat. no. R3142S), SphI-HF (New England Biolabs, cat. no. R3182S), XhoI (New England Biolabs, cat. no. R0146S) Restriction enzyme buffers (New England Biolabs): NEB3.1

(13) G-Protein-Coupled Receptor SRBC-48 Protects against Dendrite Degeneration and Reduced Longevity Due to InfectionCell ReportsMay 19, 2020Supender Kaur, Alejandro Aballayenzyme,NEW ENGLAND Biolabs,Catalog No. R3138S SphI-HF Restriction enzyme,NEW ENGLAND Biolabs,Catalog No. R3182S BamHI Restriction enzyme,NEW ENGLAND Biolabs,Catalog No. R3136S T4 DNA Ligase,NEW ENGLAND Biolabs,Catalog

(14) The Vibrio cholerae MARTX toxin silences the inflammatory response to cytoskeletal damage before inducing actin cytoskeleton collapseScience SignalingJanuary 16, 2020Patrick J. Woida, Karla J. F. Satchellare listed in Table S4. Fragments were cloned into pDS132 digested with SphI (New England Biolabs, #R3182S) using Gibson Assembly (New England Biolabs, #E2611S) according to manufacturer’s instructions. Plasmids

(15) A Doxycycline-Inducible System for Genetic Correction of iPSC Disease ModelsMethods in Molecular BiologyMay 26, 2015Xiuli Sim, Fabian L. Cardenas-Diaz, Deborah L. French, Paul Gadue#22075) with BamHI restriction enzyme. Restriction enzymes: SphI-HF® (New England Biolabs Inc. Cat. no. R3182S).BamHI (New England Biolabs Inc. Cat. no. R0136S). Standard Southern blotting reagents. 2.2 Cell Culture Media

Be the First to Write a Review!

SphI-HF

SphI-HF from New England Biolabs

Description

Sequence

New England Biolabs

Citations (15)

Reviews

SphI-HF from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map