psiCHECK-2 Vector from Promega

Product Specs
ItempsiCHECK-2 Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberC8021
Hostmammalian cell line of choice
PromoterSV40 early enhancer/promoter
ResistanceAmp
Quantity20microg
Molecular Weight~78kDa
Nucleic Acid TypeExpression Vectors
Expression System (standardized)Mammalian
Selectable Marker (standardized)Ampicillin

Specifications/Features

Vector Size:
~6.3 kb

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Identification of Tumor-Suppressive miR-139-3p-Regulated Genes: TRIP13 as a Therapeutic Target in Lung AdenocarcinomaCancers (Basel)November 24, 2023Yoko Hagihara, Yuya Tomioka, Takayuki Suetsugu, Masahiro Shinmura, Shunsuke Misono, Yusuke Goto, Naoko Kikkawa, Mayuko Kato, Hiromasa Inoue, Keiko Mizuno, et al.directly to the 3′UTR of the TRIP13 gene. The miR-139-3p-binding sequence cloned into the psiCHEK2 vector (C8021; Promega, Madison, WI, USA) is shown in Figure S1. The dual-luciferase reporter assay procedure was

(2) Identification of miR-128 Target mRNAs That Are Expressed in B Cells Using a Modified Dual Luciferase VectorBiomoleculesOctober 13, 2023Sandra Schreiber, Patrick Daum, Heike Danzer, Manuela Hauke, Hans-Martin Jäck, Jürgen Wittmannenzyme-compatible dual luciferase vectors for pRL-TK (Promega), the psiCHECK-2 vector (Promega, Cat. No. C8021) was modified as follows: first, the vector was cleaved at the XbaI site downstream of the Firefly luciferase

(3) Asymmetric trichotomous partitioning overcomes dataset limitations in building machine learning models for predicting siRNA efficacyMolecular Therapy - Nucleic AcidsSeptember 12, 2023Kathryn R. Monopoli, Dmitry Korkin, Anastasia Khvorovaconstructed with fusions of the 20-nt targeting region sequences inserted into a psiCHECK-2 vector (Promega, C8021).

(4) Minichromosome maintenance proteins in lung adenocarcinoma: Clinical significance and therapeutic targetsFEBS Open BioSeptember 1, 2023Kengo Tanigawa, Yuya Tomioka, Shunsuke Misono, Shunichi Asai, Naoko Kikkawa, Yoko Hagihara, Takayuki Suetsugu, Hiromasa Inoue, Keiko Mizuno, Naohiko Seki(UTRs) of MCM2 and the deletion‐type sequences lacking them were cloned into the psiCHECK‐2 vector (C8021; Promega, Madison, WI, USA). The procedures for transfection and dual‐luciferase reporter assays were

(5) Exosomal miRNA-223-3p derived from tumor associated macrophages promotes pulmonary metastasis of breast cancer 4T1 cellsTranslational OncologySeptember 1, 2023Ziyuan Wang, Chen Zhang, Jian Guo, Wei Wang, Qin Si, Chong Chen, Yunping Luo, Zhaojun Duansequence predicted to be bound by miR-223-3p in the 3’UTR of Cbx5 was cloned into psiCHECK-2 vector (C8021, Promega), locating at the 3’UTR of synthetic renilla luciferase gene. A mutant vector of which the

(6) Comparative analysis of the myoglobin gene in whales and humans reveals evolutionary changes in regulatory elements and expression levelsPLOS ONEAugust 29, 2023Charles Sackerson, Vivian Garcia, Nicole Medina, Jessica Maldonado, John Daly, Rachel CartwrightpSV-Rluc renilla luciferase internal standard vector was derived from psiCHECK-2 (Promega, catalog # C8021) by digestion with EagI to remove the firefly luciferase gene, leaving the renilla luciferase gene driven

(7) The oncogene Musashi1 encodes novel miRNAs in breast cancerSci RepAugust 22, 2023Liana Lachinani, Mahboobeh Forouzanfar, Kianoush Dormiani, Bahram Mohammad Soltani, Kamran Dolatshahi, Sayyed Mohammadreza Hakimian, Sadat Dokanehiifard, Mohammad Hossein Nasr-EsfahanipBud-EGFP vector at SalI and XbaI sites (Table 1B). For dual luciferase assay, psiCHECK2 (Cat. No. C8021; Promega) reporter vectors containing wild-type or mutant 3′UTR regions of the target genes were constructed.

(8) Deletion of miR-146a enhances therapeutic protein restoration in model of dystrophin exon skippingbioRxivAugust 12, 2023Nikki M. McCormack, Kelsey A. Calabrese, Christina M. Sun, Christopher B. Tully, Christopher R. Heier, Alyson A. Fiorillowas gel purified, digested with XhoI and NotI, and ligated into the pSiCheck2 plasmid (Promega Cat #C8021) where renilla luciferase is upstream of the 3’UTR and firefly luciferase is driven by a separate promoter

(9) Loss of Paip1 causes translation reduction and induces apoptotic cell death through ISR activation and Xrp1Cell Death DiscovAugust 5, 2023Maoguang Xue, Fei Cong, Wanling Zheng, Ruoqing Xu, Xiaoyu Liu, Hongcun Bao, Ying Ying Sung, Yongmei Xi, Feng He, Jun Ma, et al.AGTAATCCATGTACAAAATAACCAAC, Xrp1-5’UTRR -CAATATTATATCTCTGGGAT) and subcloned into the psiCHECK2 vector (Promega, C8021) upstream of Renilla-luciferase. Briefly, the psiCHECK2 vector was first linearized by Nhe I (NEB, R3131V).

(10) The Molecular Pathogenesis of Tumor-Suppressive miR-486-5p and miR-486-3p Target Genes: GINS4 Facilitates Aggressiveness in Lung AdenocarcinomaCellsJuly 18, 2023Yuya Tomioka, Takayuki Suetsugu, Naohiko Seki, Kengo Tanigawa, Yoko Hagihara, Masahiro Shinmura, Shunichi Asai, Naoko Kikkawa, Hiromasa Inoue, Keiko MizunoDual-Luciferase Reporter Assays The following two sequences were cloned into the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA): the wild-type sequence of the 3′-untranslated regions (UTRs) of GINS4 and

(11) Stimulation of ectopically expressed muscarinic receptors induces IFN-γ but suppresses IL-2 production by inhibiting activation of pAKT pathways in primary T cellsProceedings of the National Academy of SciencesJune 20, 2023Trang T. T. Nguyen, Wen Lu, Wandi S. Zhu, K. Mark Ansel, Hong-Erh Liang, Arthur WeissmRNA. Murine 3′UTR of IL-2 mRNA or IFN-γ mRNA was cloned into the dual-luciferase vector (psicheck2) (Promega, C8021) using XhoI and NotI sites. The Psicheck2 plasmid containing 3′UTR of IL-2 or IFN-γ mRNA was transduced

(12) Selective binding of retrotransposons by ZFP352 facilitates the timely dissolution of totipotency networkNature CommunicationsJune 20, 2023Zhengyi Li, Haiyan Xu, Jiaqun Li, Xiao Xu, Junjiao Wang, Danya Wu, Jiateng Zhang, Juan Liu, Ziwei Xue, Guankai Zhan, et al.pGL4.23 luciferase reporter plasmid containing the SV40 mini-promoter, or the psi-CHECK-2 (Promega, C8021) luciferase reporter plasmid. Luciferase reporter plasmids were transfected into HEK293T cells together

(13) Synthetic miR-21 decoy circularized by tRNA splicing mechanism inhibited tumorigenesis in glioblastoma in vitro and in vivo modelsMolecular Therapy - Nucleic AcidsJune 13, 2023Hadi Bayat, Mohammad Hossein Pourgholami, Saeid Rahmani, Safura Pournajaf, Seyed Javad Mowlaof the RNA decoys, TIMP3 3′-UTR was amplified and cloned into a psiCHECKTM-2 vector (Promega; Cat. #C8021) between XhoI (Thermo Fisher Scientific; Cat. #ER0691) and NotI (named pTIMP3-UTR).24 To construct the

(14) Genome-wide CRISPR/Cas9 screening identifies a targetable MEST-PURA interaction in cancer metastasisEBioMedicineJune 1, 2023Xu WW, Liao L, Dai W, Zheng CC, Tan XP, He Y, Zhang QH, Huang ZH, Chen WY, Qin YR, et al.of MEST. The MEST 3′-UTR was cloned into the psiCHECK-2 reporter vector (Promega, Madison, WI, USA, C8021), and mutant variants of the MEST 3′-UTR were created using the QuikChange Lightning Site-Directed Mutagenesis Kit

(15) LncRNA FALEC increases the proliferation, migration and drug resistance of cholangiocarcinoma through competitive regulation of miR-20a-5p/SHOC2 axisAging (Albany NY)May 15, 2023Haiming Du, Senlin Hou, Lichao Zhang, Chao Liu, Tingting Yu, Wei Zhangcells. Dual-luciferase reporter assay We obtained the cDNA clone of FALEC from NCBI (Gene ID: 100874054). The plasmid (C8021, psiCHECK™-2 Vectors, Promega) containing the full length wild-type (WT) or mutant (Mut) FALEC miRNA

(16) The ribosome-associated protein RACK1 represses Kir4.1 translation in astrocytes and influences neuronal activityCell ReportsMay 1, 2023Marc Oudart, Katia Avila-Gutierrez, Clara Moch, Elena Dossi, Giampaolo Milior, Anne-Cécile Boulay, Mathis Gaudey, Julien Moulard, Bérangère Lombard, Damarys Loew, et al.control cassette in frame with both the GFP and mCherry coding sequences. The psiCHECK-2 vector (Promega, C8021) was used to build the dual luciferase reporters with Kcnj10 UTRs. The UTR sequences of mouse Kcnj10

(17) miR-34c-3p Regulates Protein Kinase A Activity Independent of cAMP by Dicing prkar2b Transcripts in Theileria annulata-Infected LeukocytesmSphereApril 20, 2023Malak Haidar, Shahin Tajeri, Laurence Momeux, Tobias Mourier, Fathia Ben-Rached, Sara Mfarrej, Zineb Rchiad, Arnab Pain, Gordon Langsleythe identified canonical miR-34c-3p seed sequence, was subcloned into the psiCHECK-2 vector (Promega; C8021) and transfected into attenuated macrophages, together with the miR-34c-3p mimic, and the Renilla luciferase

(18) RNAi-mediated rheostat for dynamic control of AAV-delivered transgenesNature communicationsApril 8, 2023Subramanian M, McIninch J, Zlatev I, Schlegel MK, Kaittanis C, Nguyen T, Agarwal S, Racie T, Alvarado MA, Wassarman K, et al.containing a fully complementary (23-mer) or partial siRNA target sites into the psiCHECK2 vector (Promega C8021) between Xho1 and Not1 restriction sites. The on-target reporter plasmids contain a single site with

(19) A diagnostic circulating miRNA signature as orchestrator of cell invasion via TKS4/TKS5/EFHD2 modulation in human gliomasJournal of Experimental & Clinical Cancer ResearchMarch 17, 2023Ana Belén Díaz Méndez, Andrea Sacconi, Elisa Tremante, Valentina Lulli, Valentina Caprara, Laura Rosanò, Frauke Goeman, Mariantonia Carosi, Marta Di Giuliani, Giulia Vari, et al.and their mutants were cloned into pGL3 Control vector (#E1741, Promega) or into psiCHECK2 vector (#C8021, Promega) downstream of the luciferase gene. Human HEK-293 T (RRID: CVCL_4U22) cells were transiently

(20) Divalent siRNAs are bioavailable in the lung and efficiently block SARS-CoV-2 infectionProceedings of the National Academy of SciencesMarch 14, 2023Vignesh N. Hariharan, Minwook Shin, Ching-Wen Chang, Daniel O’Reilly, Annabelle Biscans, Ken Yamada, Zhiru Guo, Mohan Somasundaran, Qi Tang, Kathryn Monopoli, et al.to 20-nucleotide long, unique regions of target mRNAs were cloned into a psiCHECK-2 vector (Promega; C8021) according to the manufacturer′s protocol. HeLa cells were transfected with reporter plasmid using Lipofectamine

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