ShortCut RNase III from New England Biolabs

Product Specs
ItemShortCut RNase III
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0245S
Quantity200 units
ApplicationsFunctional Genomics
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) DNA polymerase ε harmonizes topological states and R-loops formation to maintain genome integrity in ArabidopsisNature CommunicationsNovember 27, 2023Qin Li, Jincong Zhou, Shuai Li, Weifeng Zhang, Yingxue Du, Kuan Li, Yingxiang Wang, Qianwen SunDNA of root tips was extracted as ssDRIP assay, the genomic DNA was firstly digested with RNase III (M0245S, NEB) at 37 °C for 2 h, then purified with HiPure Gel Pure DNA Mini Kit (D2111-03, Magen). After quantification

(2) SIRT1 Prevents R-Loops during Chronological Aging by Modulating DNA Replication at rDNA LociCellsNovember 15, 2023Bhushan L. Thakur, Nana A. Kusi, Sara Mosavarpour, Roger Zhu, Christophe E. Redon, Haiqing Fu, Anjali Dhall, Lorinc S. Pongor, Robin Sebastian, Fred E. Indig, et al.MA, USA, cat# EN0531) and shortcut RNase III (2 units; New England Biolabs, Ipswich, MA, USA; Cat# M0245L) and re-purified via phenol/chloroform/isoamyl alcohol (25:24:1) extraction. In total, 200 ng of genomic

(3) Loss of ADAR1 in macrophages in combination with interferon gamma suppresses tumor growth by remodeling the tumor microenvironmentJournal for ImmunoTherapy of CancerNovember 7, 2023Weiwei Lin, Yikai Luo, Jie Wu, Haowan Zhang, Ge Jin, Chahua Guo, Hang Zhou, Han Liang, Xiaoyan Xu8944-GD-025), Recombinant Mouse IL-18 Binding Protein Isoform d (Novoprotein, CM45), ShortCut RNase III (NEB, M0245S), poly (I:C) (GlpBio, GC14710). Anti-ADAR1 antibody (Santa Cruz, sc-73408), anti-ADAR1 antibody (Santa Cruz

(4) Flap endonuclease Rad27 cleaves the RNA of R-loop structures to suppress telomere recombinationNucleic Acids ResearchMay 22, 2023Chia-Chun Liu, Hsin-Ru Chan, Guan-Chin Su, Yan-Zhu Hsieh, Kai-Hang Lei, Tomoka Kato, Tai-Yuan Yu, Yu-wen Kao, Tzu-Hao Cheng, Peter Chi, et al.fragmented into ∼500 bp by sonication (Qsonica Sonicator Q700) and then treated with 2 U of RNase III (NEB #M0245). Finally, 10 μg of RNase A and 25 U of RNase H were added in the RNase H + A groups and incubated at

(5) PARP1 associates with R-loops to promote their resolution and genome stabilityNucleic acids researchMarch 21, 2023Laspata N, Kaur P, Mersaoui SY, Muoio D, Liu ZS, Bannister MH, Nguyen HD, Curry C, Pascal JM, Poirier GG, et al.3 mM MgCl2 with 1:200 dilutions of RNase T1 (EN0541; Thermo Fisher Scientific), ShortCut RNase III (M0245S; New England Biolabs), and/or ecRNH and incubated for 1 h at 37°C. Coverslips were subsequently washed

(6) Protocol for the analysis of double-stranded RNAs in virus-infected insect cells using anti-dsRNA antibodiesSTAR ProtocolsMarch 1, 2023Isaque J.S. de Faria, Jean-Luc Imler, João T. MarquesRPN3004 RNase A/T1 Mix,Thermo Fisher Scientific,Cat# EN0551 ShortCut RNase III,New England Biolabs,Cat# M0245S "Platinum Taq DNA Polymerase, DNA-free",Thermo Fisher Scientific,Cat# 15966005 Nuclease-free H2O,Thermo Fisher Scientific

(7) Approach to Measuring the Effect of PARP1 on RNA Polymerase II Elongation RatesMethods in molecular biology (Clifton, N.J.)January 1, 2023Dhahri H, Matveeva E, Fondufe-Mittendorf Y.instrument. MEGAscript RNAi Kit instructions (Thermo Fisher, AM1626). ShortCut RNase III Digestion (NEB, M0245). 3 M Sodium Acetate (NaOAc) (dissolve 246.1 g of sodium acetate in 800 mL of sterile distilled water

(8) Flap Endonuclease 1 Endonucleolytically Processes RNA to Resolve R-Loops through DNA Base Excision RepairGenes (Basel)December 29, 2022Eduardo E. Laverde, Aris A. Polyzos, Pawlos P. Tsegay, Mohammad Shaver, Joshua D. Hutcheson, Lata Balakrishnan, Cynthia T. McMurray, Yuan Liupretreated with single- and double-strand RNA nucleases RNase III (0.05 U/μL) (New England BioLabs, cat# M0245S, Ipswich, MA, USA) and RNase T1 (0.04 U/μL) (Sigma Aldrich, cat# R1003) in 1 x Shortcut buffer (New

(9) Chemical reversible crosslinking enables measurement of RNA 3D distances and alternative conformations in cellsNature CommunicationsDecember 1, 2022Ryan Van Damme, Kongpan Li, Minjie Zhang, Jianhui Bai, Wilson H. Lee, Joseph D. Yesselman, Zhipeng Lu, Willem A. Velema4 °C. RNA fragmentation A 10 μg of cross-linked RNA was fragmented using 10 μl of RNase III (NEB, M0245) with 5mM MnCl2 and 1× supplied shortcut buffer at 37 °C for 5 mins. After incubation, an equal volume

(10) AATF /Cheâ€1 localizes to paraspeckles and suppresses Râ€loops accumulation and interferon activation in Multiple MyelomaThe EMBO JournalNovember 17, 2022Tiziana Bruno, Giacomo Corleone, Valeria Catena, Clelia Cortile, Francesca De Nicola, Francesca Fabretti, Svitlana Gumenyuk, Francesco Pisani, Andrea Mengarelli, Claudio Passananti, et al.T9033 DMSO,Santacruz,Cat# sc‐358801 RNase T1,ThermoFisher,Cat# EN0541 Shortcut RNase III,New England Biolabs,Cat# M0245S RNase H1,New England Biolabs,Cat# M297S Software,Software,Software ImageJ,, Zeiss Zen,Zeiss (GERMANY), FASTQC

(11) Integrative analysis and prediction of human R-loop binding proteinsG3 Genes|Genomes|GeneticsJuly 29, 2022Arun Kumar, Louis-Alexandre Fournier, Peter C Stirlingtreated with RNaseH (New England Biolabs, M0297S) for 2 h, or ShortCut RNaseIII (New England Biolabs, M0245S) or RNaseT1 (ThermoFisher Scientific, EN0541) for 30 min at 37°C after permeabilization before blocking. Immunofluorescence For

(12) Foliar Infiltration of Virus-Derived Small Hairpin RNAs Triggers the RNAi Mechanism against the Cucumber Mosaic VirusInternational Journal of Molecular SciencesApril 29, 2022Bernardo Villegas-Estrada, Manuel Alejandro Sánchez, Arnubio Valencia-Jiménezproduct was digested with DNAse I (Cat# M0303, New England Biolabs, Ipswich, MA, USA) and RNase (Cat# M0245S, New England Biolabs, Ipswich, MA, USA) following the manufacturer’s instructions. After digestion,

(13) PARP inhibition impedes the maturation of nascent DNA strands during DNA replicationNature structural & molecular biologyApril 1, 2022Vaitsiankova A, Burdova K, Sobol M, Gautam A, Benada O, Hanzlikova H, Caldecott KW.µg of RNase A (Thermo Fisher Scientific, EN0531) and 0.013U ShortCut RNase III (New England Biolabs, M0245S) in the CutSmart restriction buffer (New England Biolabs, B7204S) for 3 h at +37 °C. The digested DNA

(14) The Characterization of a Novel Virus Discovered in the Yeast Pichia membranifaciensVirusesMarch 13, 2022Mark D. Lee, Jack W. Creagh, Lance R. Fredericks, Angela M. Crabtree, Jagdish Suresh Patel, Paul A. Rowleywere incubated with ten units of ShortCut® RNase III (New England Biolabs, Ipswich, MA, USA; catalog #M0245) in the buffer conditions recommended by the manufacturer for 20 min at 37 °C. The reaction was stopped

(15) Convergent Adaptation of Ootheca Formation as a Reproductive Strategy in PolyneopteraMolecular Biology and EvolutionMarch 2, 2022Erxia Du et al.Zhu et al. 2020). The siRNAs were obtained by digesting dsRNAs with ShortCut RNase III (catalog no. M0245S, New England Biolabs) to ∼21 nt fragments (siRNAs). Briefly, 40 µg of dsRNA was digested with ShortCut RNase III

(16) dsRNA Induced IFNβ-MMP13 Axis Drives Corneal Wound HealingInvestigative ophthalmology & visual scienceFebruary 1, 2022Lan X, Zhang W, Zhu J, Huang H, Mo K, Guo H, Zhu L, Liu J, Li M, Wang L, et al.depth of the pathology were recorded. For dsRNA digestion, RNase III (0.6 U/µL (New England Biolabs, M0245S) was applied to the right eyes of the mice by subconjunctival injection (5 µL) twice per day (Supplementary

(17) Absolute quantitation of individual SARS-CoV-2 RNA molecules provides a new paradigm for infection dynamics and variant differenceseLifeJanuary 20, 2022Jeffrey Y Lee et al.or 2× SSC. For RNase digestion experiments, RNaseT1 (Thermo Fisher, EN0541, 100 U/ml) or RNaseIII (M0245S, NEB, 20 U/ml) was used to degrade ssRNA and dsRNA, respectively. Permeabilised cells were treated with

(18) A rapid method to visualize human mitochondrial DNA replication through rotary shadowing and transmission electron microscopyNucleic Acids ResearchDecember 2, 2021Martin Kosar, Daniele Piccini, Marco Foiani, Michele Giannattasiothe digestion, if the genomic DNA contains traces of dsRNA, 1 μl of RNase III (New England Biolabs, #M0245S) (2 units/μl diluted 1:100 in autoclaved sterile Milli-Q water) can be added to the digestion mix for

(19) Catalytically inactive, purified RNase H1: A specific and sensitive probe for RNA–DNA hybrid imagingJournal of Cell BiologySeptember 6, 2021Magdalena P. Crossley, Joshua R. Brickner, Chenlin Song, Su Mon Thin Zar, Su S. Maw, Frédéric Chédin, Miaw-Sheue Tsai, Karlene A. Cimprichacid substrates (2 μl) were added to make a 10-µl reaction containing enzymes (2 units of RNase III [M0245L; New England Biolabs], 1,000 units RNase T1 [EN0542; Thermo Fisher Scientific], a combination of both,

(20) BRCA2 deficiency reveals that oxidative stress impairs RNaseH1 function to cripple mitochondrial DNA maintenanceCell ReportsAugust 3, 2021Xavier Renaudin, Miyoung Lee, Mona Shehata, Eva-Maria Surmann, Ashok R. VenkitaramanH1009 Etomoxir,Sigma,Cat#: E1905 37% formaldehyde solution,Sigma,Cat#: F8775 RNase H,NEB,Cat#: M0297L RNaseIII,NEB,Cat#: M0245S RNaseT1,ThermoScientific,Cat#: EN0541 DAPI,Sigma,Cat#: D9542 "cOmplete, EDTA-free Protease Inhibitor

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ShortCut RNase III from New England Biolabs

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ShortCut RNase III from New England Biolabs

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