S1 Nuclease from Promega

Product Specs
ItemS1 Nuclease
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM5761
Quantity10,000u
Concentration20–100u/µl

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to produce 1µg of acid-soluble material per minute at 37°C in 30mM sodium acetate (pH 4.6 at 25°C), 50mM NaCl, 1mM ZnCl2, 0.5mg/ml denatured calf thymus DNA and 5% glycerol.

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Mosquito transgenerational antiviral immunity is mediated by vertical transfer of virus DNA sequences and RNAiiScienceJanuary 19, 2024Julio Rodriguez-Andres, Jason Axford, Ary Hoffmann, John Kneale Fazakerley1,Merk,215643 Glutamax,Gibco,35050-061 GoTaq® Hot Start polymerase,Promega,M5005 Endonuclease III,Promega,M5761 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, Superscript

(2) Rapid ex vivo reverse genetics identifies the essential determinants of prion protein toxicityBrain PathologyMarch 1, 2023Regina R. Reimann, Martina Puzio, Antonella Rosati, Marc Emmenegger, Bernard L. Schneider, Pamela Valdés, Danzhi Huang, Amedeo Caflisch, Adriano Aguzzi(Macher‐Nagel; ref 740952.5) following the manufacturer's instructions. After S1 nuclear digestion (Promega, M5761), qPCR was performed for the woodchuck hepatitis virus post‐transcriptional regulatory element (WRPE,

(3) TREX1 Deficiency Induces ER Stress-Mediated Neuronal Cell Death by Disrupting Ca2+ HomeostasisMolecular NeurobiologyMarch 1, 2022Debasish Halder et al.treatment, 500 U/mL of nuclease was added during RNase treatment with the supplied S1 buffer (Promega M5761). The SH-SY5Y cells transfected with oligonucleotides displayed high amounts of ssDNA puncta, whereas

(4) A DCL3 dicing code within Pol IV-RDR2 transcripts diversifies the siRNA pool guiding RNA-directed DNA methylationeLifeJanuary 31, 2022Andrew Loffer, Jasleen Singh, Akihito Fukudome, Vibhor Mishra, Feng Wang, Craig S Pikaarddrug",RNase Inhibitor (Murine),NEB,Cat # M0314, "Chemical compound, drug",S1 nuclease,Promega,Cat # M5761, "Chemical compound, drug","Proteinase K, RNA grade",Invitrogen,Cat # 25530049, "Chemical compound,

(5) Viral Double-Stranded RNA Detection by DNase I and Nuclease S1 digestions in Leishmania parasitesBIO-PROTOCOLMay 5, 2020Nathalie Isorce, Nicolas Faselcatalog number: 10 776 785 001) stored at -20 °C 26. Nuclease S1 (#E576) (Promega, catalog number: M5761) stored at -20 °C Note: Pay attention to the fact that the concentration is specific to each tube and

(6) Development of an in vitro pre-mRNA splicing assay using plant nuclear extractPlant MethodsDecember 1, 2018Mohammed Albaqami, Anireddy S. N. Reddy[32P]-labelled RNA/oligo DNA was digested with S1 nuclease (100U) (Promega, Madison, WI; catalog number: M5761) in 1× S1 nuclease buffer (provided with the enzyme) for 1 h at 37 °C. The undigested [32P]-labelled

(7) Topoisomerase 3α Is Required for Decatenation and Segregation of Human mtDNAMolecular cellJanuary 4, 2018Nicholls TJ, Nadalutti CA, Motori E, Sommerville EW, Gorman GS, Basu S, Hoberg E, Turnbull DM, Chinnery PF, Larsson NG, et al.Proteinase K,Thermo Fisher,Cat#AM2548 T7 endonuclease I,New England Biolabs,Cat#M0302 S1 nuclease,Promega,Cat#M5761 E. coli Topo I,New England Biolabs,Cat#M0301 Human TopIIα,Inspiralis,Cat#HT201 Human TopIIβ,Inspiralis,Cat#HTB201 E. coli

(8) Modeling of TREX1-Dependent Autoimmune Disease using Human Stem Cells Highlights L1 Accumulation as a Source of NeuroinflammationCell stem cellSeptember 7, 2017Thomas CA, Tejwani L, Trujillo CA, Negraes PD, Herai RH, Mesci P, Macia A, Crow YJ, Muotri AR.treatment, 500 U/mL of nuclease was added during RNase treatment with the supplied S1 buffer (Promega M5761). The NPCs transfected with oligonucleotide displayed high levels of ssDNA puncta, whereas the NPCs

(9) Promoter H3K4 methylation dynamically reinforces activation-induced pathways in human CD4 T cellsGenes and immunityJuly 1, 2016LaMere SA, Thompson RC, Komori HK, Mark A, Salomon DR.converted to cDNA using the Ovation RNA-Seq system (NuGEN) followed by S1 endonuclease digestion (Promega, M5761) as previously described.49 Digested cDNA libraries were then end-repaired and A-tailed. Indexed adapters

(10) Euler buckling and nonlinear kinking of double-stranded DNANucleic Acids ResearchNovember 1, 2013Alexander P. Fields, Elisabeth A. Meyer, Adam E. Cohen25°C at a rate of 1°C/45 s. Reactions were initiated by addition of 356 units S1 nuclease (Promega M5761) in 10 µl of identical buffer. High S1 concentration was required because the buffer differed from the

(11) Recombination of 5' subgenomic RNA3a with genomic RNA3 of Brome mosaic bromovirus in vitro and in vivoVirologyOctober 29, 2010Joanna Sztuba-Solinska, Aleksandra Dzianott, Jozef J. Bujarskidetect double-stranded (ds) RNA, the RNA products were treated with S1 nuclease (Promega Corp., cat # M5761) as recommended by the manufacturer, followed by electrophoresis in denaturing agarose gels. The in vitro

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S1 Nuclease

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Specifications/Features

Promega

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S1 Nuclease from Promega

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