Molecular Beacons from MilliporeSigma

Product Specs
ItemMolecular Beacons
CompanyMilliporeSigma
Price Supplier Page
Catalog NumberMolecular Beacons
ApplicationsPCR

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MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

Company Profile

Website: www.SigmaAldrich.com/

(1) Cup is essential for oskar mRNA translational repression during early Drosophila oogenesisRNA BiolAugust 9, 2023Livia V. Bayer, Samantha Milano, Stephen K. Formel, Harpreet Kaur, Rishi Ravichandran, Juan A. Cambeiro, Lizaveta Slinko, Irina E. Catrina, Diana P. Bratucontaining a cocktail of five osk-specific molecular beacons at a concentration of 200 ng/µl of each probe. Molecular beacons were designed, synthesized, labelled, and purified as we previously described (Supplemental_Methods_S2)

(2) Ultrafast Detection of Exosomal RNAs via Cationic Lipoplex Nanoparticles in a Micromixer Biochip for Cancer DiagnosisACS Applied Nano MaterialsMarch 26, 2021Yunchen Yang, Eric Kannisto, Santosh K. Patnaik, Mary E. Reid, Lei Li, Yun Wu(Philadelphia, PA). Rhodamine B (AC29657–0250) was purchased from Fisher Scientific (Waltham, MA). Molecular beacons (MBs) detecting miR-21 and TTF-1 mRNA were synthesized by Sigma-Aldrich. The sequence of FAM-miR-21

(3) Non-invasive Detection of Exosomal MicroRNAs via Tethered Cationic Lipoplex Nanoparticles (tCLN) Biochip for Lung Cancer Early DetectionFrontiers in GeneticsMarch 20, 2020Chang Liu, Eric Kannisto, Guan Yu, Yunchen Yang, Mary E. Reid, Santosh K. Patnaik, Yun WuTable S1 for details of molecular beacons. Preparation of Cationic Lipoplex Nanoparticles Containing Molecular Beacons (CLN-MB) A mixture of FAM labeled MB and Cy5 labeled MB (mass ratio = 1:1) in 1X DPBS (Thermo Fisher Scientific

(4) EPIDEMIOLOGY OF EMYDOIDEA HERPESVIRUS 1 IN FREE-RANGING BLANDING'S TURTLES (EMYDOIDEA BLANDINGII) FROM ILLINOISJournal of Zoo and Wildlife MedicineSeptember 23, 2019Dana M. Lindemann, Matthew C. Allender, Dan Thompson, Gary A. Glowacki, Erin M. Newman, Laura A. Adamovicz, Rebecca L. SmithReal-time fluorescence reporters include DNA binding dyes (SYBR® Green I) and probe-based chemistries (TaqMan®, Molecular Beacons, ScorpionsTM) (Sigma-Aldrich 2008; Pestana et al. 2010). Both dye-based and probe-based qPCR utilize

(5) Extracellular mRNA detected by molecular beacons in tethered lipoplex nanoparticles for diagnosis of human hepatocellular carcinomaPLOS ONEJune 7, 2018Xinmei Wang et al.combination with our TLN biochip for detecting the genetic materials in EVs as biomarkers. MB design Molecular beacons that detected AFP and GPC-3 mRNAs were custom synthesized by Sigma-Aldrich (St. Louis, MO). AFP-174:

(6) Human endogenous retrovirus (HERV) expression is not induced by treatment with the histone deacetylase (HDAC) inhibitors in cellular models of HIV-1 latencyRetrovirologyDecember 1, 2016Tara Hurst, Matthew Pace, Aris Katzourakis, Rodney Phillips, Paul Klenerman, John Frater, Gkikas Magiorkinisrecently active clade of HK2 and the co-opted copies of HERV-W and HERV-FRD, we designed primers and Molecular Beacons probes using Beacon Designer software (Premier Bio-Soft) and had the oligos and probes synthesized by

(7) Nampt/PBEF/visfatin serum levels: a new biomarker for retinal blood vessel occlusionsClinical OphthalmologyApril 22, 2015Simon Kaja et al.St Louis, MO, USA) to determine the presence of T1001G and C1535T polymorphisms in the PBEF1 gene. Molecular beacons were labeled with either 6-carboxyfluorescein (6FAM) or hexachloro-6-carboxyfluorescein (HEX) at the

(8) Detection of Extracellular RNAs in Cancer and Viral Infection via Tethered Cationic Lipoplex Nanoparticles Containing Molecular BeaconsAnalytical ChemistryDecember 3, 2013Yun Wu et al.[3,4]imidazol-4-yl]-N-(29-mercapto-3,6,9,12,15,18-hexaoxanonacosyl) pentanamide, was purchased from Nanoscience Instruments (Phoenix, AZ). Molecular beacons (MBs) were custom synthesized by Sigma-Aldrich (St. Louis, MO) and TIB MOLBIOL, LLC (Adelphia, NJ).

(9) Low oxygen tension increased fibronectin fragment induced catabolic activities - response prevented with biomechanical signalsArthritis Research & TherapyOctober 25, 2013Eleanor Parker, Sandrine Vessillier, Belinda Pingguan-Murphy, Wan Abas, Dan L Bader, Tina T Chowdhury(SigmaAldrich Ltd, Gillingham, Dorset, UK). Real-time quantitative polymerase chain (qPCR) assays coupled with Molecular Beacons were performed in 25-μl reaction mixtures containing 1 μl cDNA, 12.5 μl JumpStart Taq PCR Master Mix,

(10) Embryonic Corneal Schwann Cells Express Some Schwann Cell Marker mRNAs, but No Mature Schwann Cell Marker ProteinsInvestigative Opthalmology & Visual ScienceSeptember 1, 2009Abigail H. Conrad, Michael Albrecht, Maya Pettit-Scott, Gary W. ConradCenter for Biotechnology Information, Bethesda, MD). PCR primers for QPCR were designed (Designer 31 Molecular Beacons Design; Sigma-Aldrich, St. Louis, MO) to amplify fragments between 80 and 150 base pairs in length and

(11) Twenty-five years of quantitative PCR for gene expression analysisBioTechniquesMay 14, 2008Heather D. VanGuilder, Kent E. Vrana, Willard M. Freemannecessary LightCycler Roche probe hybridization yes not necessary LUX Invitrogen hairpin probe hybridization yes necessary Molecular Beacons SigmaAldrich, IDT hairpin-loop hybridization yes necessary SYBR Green Multiple intercalating

(12) Development of a Facile Fluorescent Assay for the Detection of 80 Mutations Within the p53 GeneMolecular MedicineMay 1, 2002Darren R. Hodgson, Carole A. Foy, Maxine Partridge, Stelios Pateromichelakis, Neil J. GibsonQiagen (Hilden, Germany). All other chemicals were from Aldrich Chemical Company Co. (Milwaukee WI). Molecular beacons were prepared by Oswel Research Products (Southampton UK). The remaining oligonucleotides were synthesized

(13) High-Throughput Methods for Detection of Genetic VariationBioTechniquesMarch 10, 2001Vessela Nedelcheva Kristensen, Dimitris Kelefiotis, Tom Kristensen, Anne-Lise Børresen-DaleDNA sequencing Invasive cleavage of nucleotide probes RAPD (AP-PCR) Ligation-based assays DT-PCR DSCA ALFP Molecular beacons CFLP, EMD Microarrays “loss of signal” Microarrays “gain of signal” FRET Minisequencing reaction

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Molecular Beacons from MilliporeSigma

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Molecular Beacons from MilliporeSigma

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