NEBNext Second Strand Synthesis (dNTP-free) Reaction Buffer from New England Biolabs

Product Specs
ItemNEBNext Second Strand Synthesis (dNTP-free) Reaction Buffer
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberB6117S
Quantity0.4 ml
ApplicationsDNA Analysis
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeBuffer
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) VarID2 quantifies gene expression noise dynamics and unveils functional heterogeneity of ageing hematopoietic stem cellsGenome BiologyJune 23, 2023Reyna Edith Rosales-Alvarez, Jasmin Rettkowski, Josip Stefan Herman, Gabrijela Dumbović, Nina Cabezas-Wallscheid, Dominic GrünM0209L), E. coli DNA ligase (NEB, M0205L), RNaseH (NEB, M0297S), and 10 × Second-Strand Buffer (NEB, B6117S) respectively. The water volume was adjusted to adequately dilute the 10x second-strand buffer. After

(2) Promoter‐pervasive transcription causes RNA polymerase II pausing to boost DOG1 expression in response to saltThe EMBO JournalMarch 1, 2023Miguel Montez, Maria Majchrowska, Michal Krzyszton, Grzegorz Bokota, Sebastian Sacharowski, Magdalena Wrona, Ruslan Yatusevich, Ferran Massana, Dariusz Plewczynski, Szymon Swiezewski,New England Biolabs,M0314L "N,N‐Dimethylformamide",Sigma‐Aldrich,227056‐250ML Na2HPO4·7H2O,Sigma‐Aldrich,30413‐500G NaCl,POCH,794121116 NEBNext Second Strand Synthesis (dNTP‐free) Reaction Buffer,New England Biolabs,B6117S NP‐40,BioShop,NON505.100 Oligo(dT),Sigma‐Aldrich,N/A PEG 6000,Sigma‐Aldrich,8.07491.1000 PEG 8000

(3) Multilayer omics analysis reveals a non-classical retinoic acid signaling axis that regulates hematopoietic stem cell identityCell Stem CellJanuary 6, 2022Katharina Schönberger et al.kit,NEB,E7805 SMARTseq v4,Takare Bio,634889 NEBNext Secondary Strand Synthesis Reaction Buffer,NEB,B6117S Nextera XT DNA Sample Preparation Kits,Illumina,1502354 Deposited data,Deposited data,Deposited data RNA Seq

(4) Genomic RNA Elements Drive Phase Separation of the SARS-CoV-2 NucleocapsidMolecular cellDecember 17, 2020Iserman C, Roden CA, Boerneke MA, Sealfon RSG, McLaughlin GA, Jungreis I, Fritch EJ, Hou YJ, Ekena J, Weidmann CA, et al.columns,QIAGEN,74204 Illustra G-50 microspin columns,GE Healthcare,GE27-5330-01 NEBNext second-strand synthesis reaction,NEB,B6117S Nextera XT,Illumina,FC-131-1024 "Amicon Ultra-4, PLGC Ultracel-PL Membrane, 10 kDa",Merck,UFC801024 ,, Deposited

(5) Upon microbial challenge, human neutrophils undergo rapid changes in nuclear architecture and chromatin folding to orchestrate an immediate inflammatory gene programGenes & DevelopmentFebruary 1, 2020Matthew Denholtz, Yina Zhu, Zhaoren He, Hanbin Lu, Takeshi Isoda, Simon Döhrmann, Victor Nizet, Cornelis MurremM dATP, dGTP, dCTP, and dUTP mix; 15 µL of 5× second strand synthesis buffer (New England Biolabs B6117S); 0.5 µL E. coli ligase (New England Biolabs M0205S); 2 µL of DNA polymerase I (New England Biolabs

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