SacI from Promega

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2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

(1) Pesticide residue exposure provides different responses of the microbiomes of distinct cultures of the stored product pest mite Acarus siroBMC MicrobiologyOctober 19, 2022Jan Hubert, Blanka Navratilova, Bruno Sopko, Marta Nesvorna, Thomas W. PhillipsPromega) according to the manufacturer’s protocol. Plasmids were linearized by SacI restriction (Cat. No. R6061, Promega) and cleaned with a Wizard SV gel and PCR Clean-Up system (Cat No. A9285). The concentration

(2) Suberanilohydroxamic acid prevents TGF-β1-induced COX-2 repression in human lung fibroblasts post-transcriptionally by TIA-1 downregulationBiochimica et Biophysica Acta (BBA) - Gene Regulatory MechanismsMay 1, 2018Alice Pasini, Oliver J. Brand, Gisli Jenkins, Alan J. Knox, Linhua Pangreverse 5′-CTCTCTCGAGTTTCCAACACAGTGTCGCAG-3′. 1 μg of purified PCR products was then digested using SacI (R6061, Promega) and XhoI (R6161, Promega) restriction enzymes following the manufacturer's instructions. Digestion

(3) Intracellular uptake of macromolecules by brain lymphatic endothelial cells during zebrafish embryonic developmenteLifeMay 12, 2017van Lessen M, Shibata-Germanos S, van Impel A, Hawkins TA, Rihel J, Schulte-Merker S.mutants was performed by PCR amplification (Table 1) followed by restriction digest with SacI (Promega, # R6061). Analysis was performed by standard gel electrophoresis. Live imaging Live imaging was carried out

(4) A novel adjuvant-free H fusion system for the production of recombinant immunogens in Escherichia coli: Its application to a 12 kDa antigen from Cryptosporidium parvumBioengineeredNovember 1, 2013Sofia J Costa, Pedro Silva, André Almeida, Antónia Conceição, Lucília Domingues, António Castroplasmid containing the cp12 gene was digested with SacI and KpnI restriction enzymes (Promega, Catalog # R6061 and R6341) and the cp12 gene was then cloned into the same restriction sites of pQE-30 (Qiagen, Catalog

SacI