Access RT-PCR Introductory System from Promega

Product Specs
ItemAccess RT-PCR Introductory System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA1260
Quantity20 reactions

Specifications/Features

Comments:
A1260

Kit Contents:
1 x 100u AMV Reverse Transcriptase , 1 x 100u Tfl DNA Polymerase, 1 x 1ml AMV/Tfl 5X Reaction Buffer, 1 x 1250µl MgSO4, 1 x 20µl dNTP Mix , 1 x 50µl Positive Control RNA with Carrier, 1 x 100µl Upstream Control Primer, 1 x 100µl Downstream Control Primer, 1 x 13ml Nuclease-Free Water

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Astragalus mongholicus Bunge and Panax notoginseng formula (A&P) improves renal mesangial cell damage in diabetic nephropathy by inhibiting the inflammatory response of infiltrated macrophagesBMC Complementary Medicine and TherapiesDecember 1, 2022Xiao Lin, Xiao-qin Lei, Jie-ke Yang, Jian Jia, Xia Zhong, Rui-zhi Tan, Li Wangtranscription was performed to obtain complementary DNA (cDNA) by using the Reverse Transcription Kit (Promega, A1260, China). Finlly, quantitative PCR was performed using Eastep qPCR Master Mix (Promega, A1260, China)

(2) Characterization of Neurodevelopmental Abnormalities in iPSC-Derived Striatal Cultures from Patients with Huntington’s DiseaseJournal of Huntington's DiseaseAugust 27, 2019Pranav P. Mathkar, Divya Suresh, James Dunn, Colton M. Tom, Virginia B. MattisPromega RQ1 DNAse (M6101), then cDNA was generated using Promega AMV reverse transcriptase system (A1260) per manufacturer’s instructions. SYBR green (Thermofisher A25780) was then used to amplify the cDNA

(3) Long non‐coding RNA PCAT6 targets miR‐204 to modulate the chemoresistance of colorectal cancer cells to 5‐fluorouracil‐based treatment through HMGA2 signalingCancer MedicineMay 1, 2019Haijun Wu, Qiongyan Zou, Hong He, Yu Liang, Mingjun Lei, Qin Zhou, Dan Fan, Liangfang Shenbinding site of miR‐204) were cloned into the pGEM‐T easy vector system (wt‐PCAT6, mut‐PCAT6; Cat. A1260, Promega, Madison, WI, USA). The primers were shown in Table S1. HEK293 cells were seeded into a 24‐well

(4) EphB2-dependent signaling promotes neuronal excitotoxicity and inflammation in the acute phase of ischemic strokeActa neuropathologica communicationsFebruary 5, 2019Ernst AS, Böhler LI, Hagenston AM, Hoffmann A, Heiland S, Sticht C, Bendszus M, Hecker M, Bading H, Marti HH, et al.37 °C. Subsequently, cDNA was synthesized using the Access Reverse Transcription PCR Kit (Promega, #A1260) and quantitative real-time PCR for the target sequences was performed in the Rotor-Gene Q (Qiagen,

(5) A Novel Probe for Spliceosomal Proteins that Induces Autophagy and Death of Melanoma Cells Reveals New Targets for Melanoma Drug DiscoveryCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacologyJanuary 1, 2019Palrasu M, Knapinska AM, Diez J, Smith L, LaVoi T, Giulianotti M, Houghten RA, Fields GB, Minond D.RNA concentration was measured by Nanodrop. RT-PCR was conducted using Access RT-PCR system (Promega #A1260) following manufacturer’s protocol using forward (5’-cct tgt agt tga gaa cca gg-3’) and reverse (5’-ggg

(6) Cloning and expression analysis of Fgf5, 6 and 7 during early chick developmentGene Expression PatternsAugust 1, 2012Megha Kumar, Susan C. ChapmanReverse transcription was carried out for 45 min at 45°C using a reverse transcription kit (Promega, A1260), followed by PCR amplification using 1 cycle of 94°C for 2 min, 30 cycles of 94°C, 59°C and 68°C for

(7) In vitro transcription and immobilized template analysis of preinitiation complexesCurrent protocols in molecular biologyJanuary 1, 2012Justin J Lin, Michael Careytranscription reaction during primer extension. These kits are available commercially through Promega (A1260). If overall signals are weak, it may be prudent to verify that the primer itself is labeled efficiently

(8) Identification of genes involved in bamboo fiber developmentGeneJanuary 6, 2011Vineeta Rai, Jayadri Sekhar Ghosh, Amita Pal, Nrisingha Dey

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