RPMI 1640 Medium from Thermo Fisher Scientific

Product Specs
ItemRPMI 1640 Medium
CompanyThermo Fisher Scientific
Price Pricing Info Get Pricing Get Quote for this Product
Catalog Number11875093
Quantity500 mL
ApplicationsCELL CULTURE
Brand/TrademarkGibco™
Regulatory StatusFor Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals
TypeMedium

Specifications/Features

Contains:
Contains L-glutamine

Amount:
500

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Characterization of the high-affinity anti-CTLA-4 monoclonal antibody JS007 for immune checkpoint therapy of cancermAbsDecember 31, 2023Jiawei Guan, Hongchuan Liu, Yan Chai, Jie Yu, Jian Yao, Jing Wang, Zhiwei Pan, Jing Zhang, Yuehua Zhou, Hui Liu, et al.for 2 days. 1 × 104 DCs and 1 × 105 CD4 + T cells were seeded in RPMI 1640 medium (Gibco, Catalog No. 11875093) in a 96-well plate and incubated with serial fivefold dilutions of JS007 antibody, ipilimumab analogue

(2) VWCE modulates amino acid-dependent mTOR signaling and coordinates with KICSTOR to recruit GATOR1 to the lysosomesNat CommunDecember 20, 2023Tianyu Zhao, Yuanyuan Guan, Chenchen Xu, Dong Wang, Jialiang Guan, Ying LiuGibco C11995500BT). PC3 was kept in Ham’s F-12K (Gibco 21127022). 22Rv1 was kept in RPMI 1640 (Gibco 11875093). Cells were maintained in the cell culture medium supplemented with 10% fetal bovine serum (FBS), 100

(3) Therapeutic Potential of Regorafenib in Cisplatin-Resistant Bladder Cancer with High Epithelial-Mesenchymal Transition and Stemness PropertiesInt J Mol SciDecember 18, 2023Feng-Che Kuan, Jhy-Ming Li, Yun-Ching Huang, Shun-Fu Chang, Chung-Sheng Shiresistance [44]. All types of cells were cultured in RPMI 1640 medium (Thermo, Waltham, MA, USA; Cat. #11875093) with 10% serum (Thermo, Waltham, MA, USA; Cat. #10270106) and 1% antibiotics (LONZA, Basel, Swiss;

(4) Microbial metabolite butyrate promotes anti-PD-1 antitumor efficacy by modulating T cell receptor signaling of cytotoxic CD8 T cellGut MicrobesDecember 18, 2023Xinhai Zhu, Ke Li, Guichao Liu, Ruan Wu, Yan Zhang, Siying Wang, Meng Xu, Ligong Lu, Peng LiT cells were generated as described below. Human PBMCs (2.5 × 106/mL) were cultured in RPMI 1640 (Gibco, 11875093) medium supplemented with 10% fetal bovine serum (FBS; Gibco, 16140071), 2 mM L-glutamine, antibiotics,

(5) Mammary γδ T cells promote IL-17A-mediated immunity against Staphylococcus aureus-induced mastitis in a microbiota-dependent manneriScienceDecember 15, 2023Na Pan, Lei Xiu, Ying Xu, Xuemei Bao, Yanchen Liang, Haochi Zhang, Bohui Liu, Yuanyu Feng, Huibo Guo, Jing Wu, et al.1×,Gibco,Cat#14040-133 FBS,BI,Cat# 04-007-1A Collagenase D,Sigma,Cat# COLLD-RO RPMI 1640 Medium,Gibco,Cat# 11875093 BSA,Solarbio,Cat# A8010 Red blood cell lysis buffer,Solarbio,Cat# R1010 PMA/Phorbol 12-myristate 13-acetate

(6) Vertical impedance electrode array for spatiotemporal dynamics monitoring of 3D cells under drug diffusion effectiScienceDecember 15, 2023Yong Qiu, Xin Liu, Yuxuan Zhu, Deming Jiang, Fengheng Li, Weijie Yu, Hao Wan, Liujing Zhuang, Yuxiang Pan, Ping Wangproteins","Chemicals, peptides, and recombinant proteins" ,, "RPMI Medium 1640 (1X), liquid + L-Glutamine",Gibco,Cat#11875093 Fetal Bovine Serum,Gibco,Cat#10099141C Penicillin–Streptomycin,Gibco,Cat#15140122 "Trypsin-EDTA (1X),

(7) Modeling RET-Rearranged Non-Small Cell Lung Cancer (NSCLC): Generation of Lung Progenitor Cells (LPCs) from Patient-Derived Induced Pluripotent Stem Cells (iPSCs)CellsDecember 15, 2023Paul Marcoux, Jin Wook Hwang, Christophe Desterke, Jusuf Imeri, Annelise Bennaceur-Griscelli, Ali G. TurhanGeltrex the day before definitive endoderm induction (DE). DE induction medium is composed of RPMI1640 (11875093; Gibco, Illkirch, France) supplemented with Glutamax (35050061; Gibco, Illkirch, France), B27 (12587010;

(8) HPV-Negative and HPV-Positive Oral Cancer Cells Stimulate the Polarization of Neutrophils towards Different Functional Phenotypes In VitroCancers (Basel)December 12, 2023Marcela Guadalupe Martínez-Barajas, Luis Felipe Jave-Suárez, Inocencia Guadalupe Ramírez-López, Mariel García-Chagollán, José Sergio Zepeda-Nuño, Adrián Ramírez-de-Arellano, Pablo César Ortiz-Lazareno, Julio César Villegas-Pineda, Ana Laura Pereira-Suárezsaline solution for 10 min at 1000 rpm and RT. Neutrophils were then resuspended in RPMI-1640 (Cat. No. 11875093, Gibco™, Waltham, MA, USA) supplemented with 10% FBS and 1% antibiotic. Finally, cell viability and

(9) Mechanistic insights into the anti-restenotic effects of HSP27 and HO1 modulated by reconstituted HDL on neointimal hyperplasiaSci RepDecember 12, 2023Ye Ji Kim, Zinah Hilal Khaleel, Myeongji Jin, Jo Woon Yi Lee, Seongchan Park, Seongmin Ga, Nam Hyeong Kim, Deok Hyang Sa, Eun Sung Kang, Seul Hee Han, et al.(CC-3162, Lonza). THP1 was purchased (40202, Korean cell line bank) and maintained with RPMI 1640 medium (11875093, Gibco), 10% heat inactivated fetal bovine serum (FBS), penicillin (100 U/ml), and streptomycin (100

(10) Adaptive DNA amplification of synthetic gene circuit opens a way to overcome cancer chemoresistanceProceedings of the National Academy of SciencesDecember 5, 2023Yiming Wan, Quanhua Mu, Rafał Krzysztoń, Joseph Cohen, Damiano Coraci, Christopher Helenek, Christopher Tompkins, Annie Lin, Kevin Farquhar, Erin Cross, et al.Edward Chu (21). Both cell lines were passaged regularly every 3 to 4 d with RPMI1640 medium (Gibco™, 11875093) supplemented with 10% FBS (Corning™, 35-010-CV) and 1% penicillin-streptomycin (Gibco™, 15140122).

(11) Protocol for PPP1R15A-inhibited mouse model establishment with subcutaneous B16F1 tumor and single-cell analysisSTAR ProtocolsDecember 1, 2023Rongjing Wang, Minghui Wang, Siyu Pei, Yuchao Zhang, Shiwei Guo, Wei Guo, Zhenchuan Wu, Hailong Wang, Yizhe Li, Yufei Zhu, et al.(https://www.biolegend.com/en-us/products/cfse-cell-division-tracker-kit-9396) and then resuspend the cells in RPMI 1640 medium (Gibco, 11875093) supplemented with 10% FBS (Biological Industries, 04-001-1A) and 1% Pen Strep (Gibco, 15140122).d.Add

(12) Macrophage LMO7 deficiency facilitates inflammatory injury via metabolic-epigenetic reprogrammingActa Pharmaceutica Sinica BDecember 1, 2023Shixin Duan, Xinyi Lou, Shiyi Chen, Hongchao Jiang, Dongxin Chen, Rui Yin, Mengkai Li, Yuseng Gou, Wenjuan Zhao, Lei Sun, et al.(C36B11), were purchased from Cell Signaling Technology. DMEM medium (11965092), RPMI-1640 medium (11875093), and 10% fetal bovine serum (FBS, 10099) were obtained from Gibco company. 1% penicillin–streptomycin

(13) A redox-based characterization of human immune cell subsets by polychromatic flow cytometrySTAR ProtocolsDecember 1, 2023Christina Pitsillidou, Ivan Muradore, Elena Pontarini, Milena Bertolotti, Alessandra Robertosolution without calcium and magnesium,Gibco,Cat#14190-144 RPMI 1640 Medium (RPMI),Thermo Fisher,Cat#11875093 "Penicillin-Streptomycin (10,000 U/mL)",Thermo Fisher,Cat#15140122 Fetal bovine serum (FBS),EuroClone,EUS00AY Distilled

(14) The formation of ubiquitin rich condensates triggers recruitment of the ATG9A lipid transfer complex to initiate basal autophagybioRxivNovember 30, 2023David G Broadbent, Colten M McEwan, Tsz-Ming Tsang, Daniel M Poole, Bradley C Naylor, John C Price, Jens Christopher Schmidt, Joshua L AndersenReagents,Source,Identifier Penicillin-Streptomycin,Gibco,15140122 Dulbecco’s modified Eagle’s medium,Gibco,11965-092 RPMI,Gibco,11875093 Fetal Bovine Serum,Genesee Scientific,25-514 Phosphate buffered saline (1X),Corning,21-040-CV PEI-MAX,Polysciences,24765-1 Polybrene,Sigma-Aldrich,TR-1003-G LICOR

(15) Genome-wide profiling of transcription factor activity in primary liver cancer using single-cell ATAC sequencingCell ReportsNovember 28, 2023Amanda J. Craig, Maruhen A. Datsch Datsch Silveira, Lichun Ma, Mahler Revsine, Limin Wang, Sophia Heinrich, Zachary Rae, Allison Ruchinskas, Kimia Dadkhah, Whitney Do, et al.recombinant proteins" Clarity Max Western ECL Blotting Substrates,Biorad,#170562 RPMI 1640 medium,Gibco,#11875093 Fetal Bovine Serum,Fisher Scientific,#SH30396.03 Pierce Bovine Serum Albumin Standard Ampules,ThermoFisher

(16) Design and deep learning of synthetic B-cell-specific promotersNucleic Acids ResearchNovember 27, 2023Zong-Heng Fu, Si-Zhe He, Yi Wu, Guang-Rong ZhaoJurkat, Clone E6-1 (ATCC, TIB-152) cells were maintained at 37°C under 5% CO2 in RPMI-1640 medium (Gibco, 11875093) supplemented with 10% FBS (Gibco, 10091148). The Neon transfection system and 100 μl kit (ThermoFisher,

(17) Blocking CD40 Alleviates Th1 and Th17 Cell Responses in Elastin Peptide-Induced Murine EmphysemaInternational Journal of Chronic Obstructive Pulmonary DiseaseNovember 23, 2023Tingting Ma, Hui Zhang, Yuqing Weng, Shudan Tang, Jinshan Mao, Xin Feng, Yuxin Zhang, Jianquan Zhangright ventricle. The lungs were then thoroughly chopped and digested at 37°C in RPMI 1640 medium (Cat#11875093, Gibco, Thermo Fisher Scientific, Massachusetts, USA) with 1 mg/mL type IV collagenase (Cat#C4-BIOC,

(18) Targeting cuproptosis by zinc pyrithione in triple-negative breast canceriScienceNovember 17, 2023Xu Yang, Li Deng, Xianhong Diao, Siyuan Yang, Li Zou, Qin Yang, Jian Li, Jianyun Nie, Lina Zhao, Baowei JiaoHCC1806, HCC1937, and MDA-MB-468 were grown in Roswell Park Memorial Institute (PRMI) 1640 medium (11875093, Gibco) with 10% fetal bovine serum (FBS). The Hs578T cells were grown in PRMI 1640 medium with 10%

(19) Nanodiscoidal Nucleic Acids for Gene RegulationACS Chemical BiologyNovember 17, 2023Radhika Sharma, Steven Narum, Shuhong Liu, Yixiao Dong, Kyung In Baek, Hanjoong Jo, Khalid Salaita(25-053-Cl) from Corning (Tewksbury, MA, USA). H1299 cells were cultured in RPMI-40 with l-Glutamine (11875093) acquired from Gibco (Waltham, MA, USA). Spheroids were initially seeded in ultralow attachment microplates

(20) Osteopontin Activation and Microcalcification in Venous Grafts Can Be Modulated by DexamethasoneCellsNovember 15, 2023Liam W. McQueen, Shameem S. Ladak, Georgia R. Layton, Kerry Wadey, Sarah J. George, Gianni D. Angelini, Gavin J. Murphy, Mustafa Zakkarsilicon sheet gasket. A reservoir containing 30 mL RPMI 1640 culture medium (ThermoFisher Scientific, 11875093), supplemented with 2% (v/v) FCS, 100 µg/mL penicillin, and 100 U/mL streptomycin attached to a closed-circuit

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3/2/2015Use of RMPI Medium 1640 in the culture of T cells Rebecca WilshusenAfter we harvest splenocytes, T cells are differentiated for 5-7 days in complete RPMI media and associated cytokines for differentiation.

RPMI 1640 Medium from Thermo Fisher Scientific

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Thermo Fisher Scientific

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RPMI 1640 Medium from Thermo Fisher Scientific

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