Fig 1: The exogenous transgenic COLDAIR produces double-stranded RNA in vivo. (A) RT-qPCR analysis of FLC mRNA and sense and antisense strands of exogenous COLDAIR in the wild-type Col-0 and in the 35S-COLDAIR transgenic line. Error bars are SD of three replicates. (B) Analysis of FLC mRNA and exogenous COLDAIR with or without RNase treatment in vitro. RNase one (Promega, M4261) is an ssRNA specific ribonuclease, and RNase III (NEB, M0245) mediates the cleavage of double-stranded RNAs. (C) Snapshots of sense and antisense FLC transcripts in the wild type and the 35S-COLDAIR transgenic plants. Sense and antisense transcripts are shown in blue and red, respectively.
Supplier Page from Promega for RNase ONE Ribonuclease
Unit Definition:One unit is defined as the amount of enzyme required to completely degrade RNA at the rate of 100ng/second at 37°C in 10mM Tris-HCl (pH 7.5 at 25°C), 5mM EDTA, 200mM sodium acetate, 0.2µg/µl RNA, 0.05% NP-40 and 2µg/µl BSA.