RNase ONE Ribonuclease from Promega

Product Specs
ItemRNase ONE Ribonuclease
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM4265
Quantity5,000u
Concentration5–10u/µl
Molecular Weight~68kDa

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to completely degrade RNA at the rate of 100ng/second at 37°C in 10mM Tris-HCl (pH 7.5 at 25°C), 5mM EDTA, 200mM sodium acetate, 0.2µg/µl RNA, 0.05% NP-40 and 2µg/µl BSA.

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Higher convergence of human-great ape enteric eukaryotic viromes in central African forest than in a European zoo: a One Health analysisNature CommunicationsJune 21, 2023Victor Narat, Maud Salmona, Mamadou Kampo, Thibaut Heyer, Abdeljalil Senhaji Rachik, Severine Mercier-Delarue, Noémie Ranger, Stephanie Rupp, Philippe Ambata, Richard Njouom, et al.technologies, #DB0715K); Benzonase® Nuclease (MERCK Millipore, # 70664-3); RNAse One TM Ribonucleas (Promega, # M4261) for 30 min, at 37 °C. Total nucleic acids were extracted using NucliSENS®easyMAG® (Biomerieux,#280130-280135)

(2) CoLoC-seq probes the global topology of organelle transcriptomesNucleic acids researchFebruary 22, 2023Jeandard D, Smirnova A, Fasemore AM, Coudray L, Entelis N, Förstner KU, Tarassov I, Smirnov A.U/ml; Thermo Fisher Scientific, Waltham, USA, Cat # EN0551), RNase One (Promega, Madison, USA, Cat# M4261), RNase T1 (Thermo Fisher Scientific, Waltham, USA, Cat # EN0541), SUPERase·In RNase Inhibitor (Thermo

(3) Polysome-CAGE of TCL1-driven chronic lymphocytic leukemia revealed multiple N-terminally altered epigenetic regulators and a translation stress signatureeLifeAugust 8, 2022Ogran A, Havkin-Solomon T, Becker-Herman S, David K, Shachar I, Dikstein R.SP1100) and purified by MPG streptavidin beads (Takara, cat. 6124A) followed by RNAse (Promega, cat. M4261) digestion to release cDNAs corresponding to the 5′ ends of the original mRNA. 5′ linkers harboring

(4) Biochemical approach for isolation of polyadenylated RNAs with bound proteins from yeastSTAR ProtocolsDecember 1, 2021Ana M. Matia-González, Ibtissam Jabre, André P. GerberRNasin® Ribonuclease inhibitor,Promega,N2511 Polyadenylic acid potassium salt (Poly(A)),Sigma,P9403 RNase ONE,Promega,M4265 peqGREEN RNA/DNA dye,Peqlab,732–3196 Precision Plus Protein Dual Color Standards,BIO-RAD,1610374 Tris,Thermo Fisher

(5) Structure of human cytomegalovirus virion reveals host tRNA binding to capsid-associated tegument protein pp150Nature CommunicationsDecember 1, 2021Yun-Tao Liu, David Strugatsky, Wei Liu, Z. Hong Zhouinstructions. Virions were processed directly with miRNeasy or were pretreated with RNase One (Promega M4261) to hydrolyze RNA molecules outside of the virion. Total RNA was eluted in 50 µl of nuclease-free water

(6) G3BP1 inhibits Cul3^SPOP to amplify AR signaling and promote prostate cancerNature communicationsNovember 18, 2021Mukhopadhyay C, Yang C, Xu L, Liu D, Wang Y, Huang D, Deonarine LD, Cyrta J, Davicioni E, Sboner A, et al.Lysates of G3BP1 and G3BP1RRM transfected cells were treated without or with 100 U RNase ONE (Promega #M4261) for 1 h at 37 °C. Efficient RNase ONE treatment was confirmed by running samples into Agilent bioanalyzer.

(7) Alternative RNA structures formed during transcription depend on elongation rate and modify RNA processingMolecular CellApril 1, 2021Tassa Saldi, Kent Riemondy, Benjamin Erickson, David L. BentleyDNAse I,NEB,M0303 ProteoGuard,Takara,635673 Formaldehyde 16% methanol free,ThermoFisher,28908 RNAseVI,Ambion,N/A RNAse One,Promega,M4261 Model cell lines,, HEK293 Flp-in T-Rex pcDNA5 Rpb1 αAmr WT,Fong et al 2014,N/A HEK293 Flp-in T-Rex

(8) Spliceosome-targeted therapies trigger an antiviral immune response in triple-negative breast cancerCellJanuary 14, 2021Elizabeth A. Bowling et al.supernatant was transferred to a new Eppendorf. Lysates were treated with or without 5U RNaseOne (Promega, M4261) for 30 minutes at room temperature. RNA was harvested using Trizol. Chloroform was added at a ratio

(9) Exogenously overexpressed intronic long noncoding RNAs activate host gene expression by affecting histone modification in ArabidopsisScientific ReportsDecember 1, 2020Zhang-Wei Liu, Nan Zhao, Yin-Na Su, Shan-Shan Chen, Xin-Jian HeRNase-treated RT-qPCR, 10 µg of total RNA was digested with RNase III (NEB, M0245S) or RNase one (Promega, M4261) according to the instruction manual. After chloroform purification, 1 µg of RNA was treated with DNase

(10) Multiplex detection of miRNAs based on aggregation-induced emission luminogen encoded microspheres†RSC AdvancesDecember 2, 2019Dan Zou et al.R-Phycoerythrin Conjugate (SAPE, 1 mg mL−1) was purchased from Thermo Fisher. RNase ONE™ ribonuclease (M4265, 10 U μL−1) was purchased from Promega. Sample preparation Preparation of AIEgens-encoded microspheres AIEgens-encoded

(11) Liver-Type Glutaminase GLS2 Is a Druggable Metabolic Node in Luminal-Subtype Breast CancerCell reportsOctober 1, 2019Lukey MJ, Cluntun AA, Katt WP, Lin MJ, Druso JE, Ramachandran S, Erickson JW, Le HH, Wang ZE, Blank B, et al.SuperScript III Reverse Transcriptase,Thermo Fisher Scientific,Cat# 18080044 RNase One Ribonuclease,Promega,Cat# M4261 CB-839,Millipore-Sigma,Cat#533717 BPTES,"Dr. Scott Ulrich, Ithaca College",N/A 968,ChemBridge Corporation,N/A Dimethyl

(12) Reaction Mechanisms of Pol IV, RDR2, and DCL3 Drive RNA Channeling in the siRNA-Directed DNA Methylation PathwayMolecular cellAugust 8, 2019Singh J, Mishra V, Wang F, Huang HY, Pikaard CS.AM9515 Ribolock RNase Inhibitor,Thermo Fisher,Cat# EO0384 RNase H,NEB,Cat# M0297S RNase I,Promega,Cat# M4261 RNase III,Epicentre,Cat# RN02950 Terminator Exonuclease,Lucigen,Cat# TER51020 Vaccinia capping system,NEB,Cat#

(13) Evaluating the susceptibility of AGO2-loaded microRNAs to degradation by nucleases in vitroMethodsJanuary 1, 2019Reyad A. Elbarbary, Lynne E. Maquatnucleases A.3.4.1. 3.5. Kits and reagents Kits and reagents 1. RNase One™ Ribonuclease (Promega; Catalog # M4261) 2. Exonuclease T (New England Biolabs; Catalog # M0265S) Purifying RNA from 3xFLAG-AGO2 IPs 3.5.1. 1. Kits

(14) The Exon Junction Complex Undergoes a Compositional Switch that Alters mRNP Structure and Nonsense-Mediated mRNA Decay ActivityCell ReportsNovember 19, 2018Justin W. Mabin, Lauren A. Woodward, Robert D. Patton, Zhongxia Yi, Mengxuan Jia, Vicki H. Wysocki, Ralf Bundschuh, Guramrit SinghIII,Invitrogen,18080044 Ribonuclease H,Promega,M4281 2X SYBR Green Master Mix,Applied Biosystems,4309155 RNase I,Promega,M4261 Ribo-Zero rRNA Removal Kit (H/M/R),Illumina,MRZH116 CircLigase,Lucigen,CL4115K ,, Deposited Data,, ,, RNA-seq

(15) Visualization of Double-Stranded RNA Colocalizing With Pattern Recognition Receptors in Arenavirus Infected CellsFrontiers in Cellular and Infection MicrobiologyJuly 24, 2018Elizabeth J. Mateer, Slobodan Paessler, Cheng Huang(1:1,000, Invitrogen). RNase III was purchased from Life technologies (AM2290) and RNase I from Promega (M4261). Immunofluorescence staining A549 cells were grown on PDL coated coverslips (Neuvitro) and infected

(16) Generation of ribosome imprinted polymers for sensitive detection of translational responsesScientific reportsJuly 26, 2017King HA, El-Sharif HF, Matia-González AM, Iadevaia V, Fowotade A, Reddy SM, Gerber AP.centrifuged twice at 18,500 × g for 10 min at 4 °C to pellet mitochondria. 10 U of RNase ONE (Promega #M4261) was added to the cleared extract and incubated for 2 min on ice, and the partial RNA digest was terminated

(17) Protein Interaction Profile Sequencing (PIP‐seq)Current Protocols in Molecular BiologyOctober 11, 2016Shawn W. Foley, Brian D. Gregoryno. AM9740) 100% EtOH (Decon Labs, cat. no. 2716) 70% EtOH RNase ONE buffer (Promega, cat. no. M4261) RNase ONE (Promega, cat. no. M4261) RNase stop buffer, 16× (see recipe) QIAzol (Qiagen, cat.

(18) Conserved mRNA-binding proteomes in eukaryotic organismsNature structural & molecular biologyDecember 1, 2015Matia-González AM, Laing EE, Gerber AP.controls were introduced at this stage: the extract was either supplemented with 100 U RNase ONE (Promega, M4265) for 2 h at 37 °C to digest RNA; or 20 mg of poly(A) (Sigma, P9403) was added to the extract for competition

(19) CAGE (Cap Analysis of Gene Expression): A Protocol for the Detection of Promoter and Transcriptional NetworksMethods in Molecular BiologyJune 4, 2014Hazuki Takahashi, Sachiko Kato, Mitsuyoshi Murata, Piero CarninciAgencourt AMPure XP Kit (BECKMAN COULTER, Cat# A63881, 60 ml) RNase ONE Ribonuclease (Promega, Cat# M4261, 1000 U) MPG Streptavidin (TAKARA, Cat# 6124A, 2ml) OliGreen ssDNA Quantitation Kit (Molecular Probes,

(20) RAMPAGE: Promoter Activity Profiling by Paired‐End Sequencing of 5′‐Complete cDNAsCurrent Protocols in Molecular BiologyNovember 11, 2013Philippe Batut, Thomas R. Gingeras(Sigma-Aldrich, 500g, Cat # S1804-500G) RNAse I digestion: – RNAse I (5-10 U/μl) (Promega, 1,000U, Cat # M4261) – EDTA 0.5M, pH8.0 (Ambion, 100 mL, Cat # AM9260G) Cap-trapping: – MPG Streptavidin beads (PureBiotech

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RNase ONE Ribonuclease from Promega

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RNase ONE Ribonuclease from Promega

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