RNase H from New England Biolabs

Product Specs
ItemRNase H
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0297S
Concentration5,000 units/ml
Quantity250 units
ApplicationsFunctional Genomics
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme that will hydrolyze 1 nmol of the RNA in [³H]-labeled poly(rA)^.poly(dT), to acid-soluble ribonucleotides in a total reaction volume of 50 µl in 20 minutes at 37°C in 1X RNase H Reaction Buffer with 10 nmol [³H]-labeled poly(rA) and 12.5 µg poly(dT).

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Function of m 5 C RNA methyltransferase NOP2 in high-grade serous ovarian cancerCancer Biology & TherapyDecember 31, 2023Shimin Yang, Dongmei Zhou, Chunxiao Zhang, Jiangdong Xiang, Xiaowei Xiconverted into DNA duplexes using E. coli DNA polymerase I (NEB, cat.m0209, USA) with RNase H (NEB, cat.m0297, USA) for two-strand synthesis. At the same time, dUTP Solution (Thermo Fisher, cat.R0133, CA, USA)

(2) Enterogenic Stenotrophomonas maltophilia migrates to the mammary gland to induce mastitis by activating the calcium-ROS-AMPK-mTOR-autophagy pathwayJ Anim Sci BiotechnolDecember 20, 2023Zhaoqi He, Caijun Zhao, Yuhong He, Zhuoyu Liu, Guyue Fan, Kun Zhu, Yiqi Wang, Naisheng Zhang, Yunhe Fu, Xiaoyu Huconverted into DNA duplexes using E. coli DNA polymerase I (NEB, cat. m0209, USA) and RNase H (NEB, cat. m0297, USA), while the duplexes were doped with dUTP Solution (R0133, Thermo Fisher Scientific, California,

(3) Blue light impairs cornea and corneal wound healing by downregulating VCAM1 partlyiScienceDecember 15, 2023Kuangqi Chen, Le Jin, Yingying Wen, Qianjie Yang, Xiang Li, Liyue Zhang, Liyin Wang, Yutong Xia, Zhitong Chen, Chen Xie, et al.synthesize cDNA. Then, E. coli DNA polymerase I (NEB, article number m0209) and RNase H (NEB, article number m0297) were used for double-stranded synthesis, and the compound double-stranded DNA and RNA were converted

(4) Fangji Dihuang formulation ameliorated DNCB-induced atopic dermatitis-like skin lesions by IL-17 signaling pathway: integrating network analysis and experimental validationFrontiers in PharmacologyNovember 27, 2023Wenting Zhao, Honghong Jiang, Yunfan Gu, Weiming Zhang, Shijie Bao, Ming Dai, Bilin Dong, Ya Yang, Ke Li, Li Qin, et al.(CAT#1896649, CA, United States). E. coli DNA Polymerase I (NEB, CAT#m0209, United States) and RNase H (CAT#m0297, NEB, United States) were utilized for two-strand synthesis in order to transform the double strands

(5) Hypoxia-induced transcriptional stress is mediated by ROS-induced R-loopsNucleic Acids ResearchNovember 27, 2023Tiffany S Ma, Katja R Worth, Conor Maher, Natalie Ng, Chiara Beghè, Natalia Gromak, Anna M Rose, Ester M Hammondrunning DNA samples on a 1.5% agarose gel. RNase H treatment was used as described by the manufacturer (M0297; NEB) for 2.5 h at 37°C for control samples. Immunoprecipitation was carried out by incubating equal

(6) Study on the Effect of Oleic Acid-Induced Lipogenic Differentiation of Skeletal Muscle Satellite Cells in Yanbian Cattle and Related MechanismsAnimals (Basel)November 23, 2023Bin Sun, Jianfu Sun, Qiang Li, Ying Wang, Enze Wang, Huaina Jin, Huan Hua, Qiyun Jin, Xiangzi LiU-labeled stranded DNAs with E. coli DNA polymerase I (NEB, cat.m0209, Ipswich, MA, USA), RNase H (NEB, cat.m0297, Ipswich, MA, USA), and dUTP Solution (Thermo Fisher, cat.R0133, Waltham, CA, USA). An A-base is then

(7) Rfoot‐seq: Transcriptomic RNase Footprinting for Mapping Stable RNA‐Protein Complexes and Rapid Ribosome ProfilingCurrent ProtocolsNovember 20, 2023Qianru Li, Emily K. Stroup, Zhe Jiand a partial Illumina read 1 sequencing adapter. RNase H (5,000 U/mL; New England BioLabs, cat. no. M0297L) PCR1 forward and reverse primers (Synthesized by IDT, see the sequence in Table 2)Ultra II Q5 master

(8) H3.1K27me1 loss confers Arabidopsis resistance to Geminivirus by sequestering DNA repair proteins onto host genomeNature CommunicationsNovember 18, 2023Zhen Wang, Claudia M. Castillo-González, Changjiang Zhao, Chun-Yip Tong, Changhao Li, Songxiao Zhong, Zhiyang Liu, Kaili Xie, Jiaying Zhu, Zhongshou Wu, et al.strand synthesis was conducted with dUTP mixture (20 mM dUTP, 10 mM dATP, dCTP, and dGTP), RNase H (NEB, M0297), DNA polymerase I (NEB, M0209) and E. coli DNA ligase (NEB, M0205). End repair was conducted with NEBNext

(9) Connecting Chromatin Structures to Gene Regulation Using Dynamic Polymer SimulationsbioRxivNovember 15, 2023Yi Fu, Finnegan Clark, Sofia Nomikou, Aristotelis Tsirigos, Timothee LionnetQuick-RNA Miniprep Kit (Zymo; #R1054S). The probes were then digested with 4 uL RNaseH (New England Biolabs M0297S) and 4 uL RNaseA (Thermo Scientific; EN0531) in RNaseH buffer using the following program: 37 °C 2 h,

(10) Epigenetic combined with transcriptomic analysis of the m6A methylome after spared nerve injury-induced neuropathic pain in miceNeural Regeneration ResearchNovember 1, 2023Fanning Zeng, Jun Cao, Zexuan Hong, Yitian Lu, Zaisheng Qin, Tao TaoU-labeled second-stranded DNAs with Escherichia coli DNA polymerase I (NEB, Cat# m0209), RNase H (NEB, Cat# m0297) and dUTP Solution (Thermo Fisher, Cat# R0133). An A-base was then added to the blunt ends of each strand,

(11) PCLLA-nanoHA Bone Substitute Promotes M2 Macrophage Polarization and Improves Alveolar Bone Repair in Diabetic EnvironmentsJournal of Functional BiomaterialsOctober 30, 2023Dandan Wang, Ling Wei, Jialin Hao, Weifeng Tang, Yuan Zhou, Chenguang Zhang, Jinming Wangusing E. coli DNA polymerase I (NEB, cat.m0209, Thermo Fisher, Waltham, MA, USA), RNase H (NEB, cat.m0297, USA), and dUTP Solution (Thermo Fisher, cat.R0133, USA). Blunt ends were generated by adding an A-base

(12) The chromatin network helps prevent cancer-associated mutagenesis at transcription-replication conflictsNature CommunicationsOctober 28, 2023Aleix Bayona-Feliu, Emilia Herrera-Moyano, Nibal Badra-Fajardo, Iván Galván-Femenía, María Eugenia Soler-Oliva, Andrés Aguileraantibody (hybridoma HB-8730). As control, samples were in vitro treated with RNase H (New England Biolabs M0297L). Finally, DNA-RNA hybrid immunoprecipitated fractions were measured by quantitative PCR (qPCR) using

(13) SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9CellOctober 26, 2023Nora Schmidt, Sabina Ganskih, Yuanjie Wei, Alexander Gabel, Sebastian Zielinski, Hasmik Keshishian, Caleb A. Lareau, Liv Zimmermann, Jana Makroczyova, Cadence Pearce, et al.V5-Trap Magnetic Agarose,Proteintech,V5TMA Triton-X 100,Sigma-Aldrich,T9284-100ML RNase H,New England Biolabs,M0297L 4x LDS sample buffer,Thermo Fisher Scientific,B0007 QX200 ddPCR EvaGreen Supermix,BioRad,1864034 QX200 droplet generation oil for EvaGreen

(14) Schlafen-5 inhibits LINE-1 retrotranspositioniScienceOctober 20, 2023Jiwei Ding, Shujie Wang, Qipeng Liu, Yuqing Duan, Tingting Cheng, Zhongjie Ye, Zhanding Cui, Ao Zhang, Qiuyu Liu, Zixiong Zhang, et al.(G418),absin,Cat:#abs812846 DNase,Takara,Cat:#2270A RNasin,Promega,Cat:#N2115 Recombinant RNase inhibitor,Takara,Cat:#2313A RNase H,New England BioLabs,Cat:#M0297L Dynabeads Protein A,Invitrogen,Cat:#10002D ,, Critical commercial assays,Critical commercial assays,Critical

(15) CEP20 promotes invasion and metastasis of non-small cell lung cancer cells by depolymerizing microtubulesScientific ReportsOctober 14, 2023Sijie Feng, Shuai Yuan, Baohua Hou, Zhiqiang Liu, Yanjun Xu, Shuangying Hao, Yunkun Lusynthesize U-labeled second-stranded DNAs with E. coli DNA polymerase I (NEB, m0209, USA), RNase H (NEB, m0297, USA), and dUTP solution (Thermo Fisher, R0133, USA). An A-base is then added to the blunt ends of each

(16) HybriSeq: Probe-based Device-free Single-cell RNA ProfilingbioRxivOctober 5, 2023Daniel Foyt, David Brown, Shuqin Zhou, Bo Huangwashing, cells were resuspended in Rnase H reaction buffer containing 20 U/mL of Rnase H enzyme (NEB M0297S). Cells were incubated for one hour at 37 °C with gentle agitation. Released probes were quantified

(17) MicroRNA-122-functionalized DNA tetrahedron stimulate hepatic differentiation of human mesenchymal stem cells for acute liver failure therapyBioactive MaterialsOctober 1, 2023Hongyan Wei, Fenfang Li, Tiantian Xue, Haixia Wang, Enguo Ju, Mingqiang Li, Yu TaoU-labeled second-stranded DNAs with E. coli DNA polymerase I (NEB, cat.m0209, USA), RNase H (NEB, cat.m0297, USA) and dUTP solution (Thermo Fisher, cat.R0133, USA). An A-base is then added to the blunt ends of

(18) Transcriptomic profiling of human granulosa cells between women with advanced maternal age with different ovarian reserveJournal of Assisted Reproduction and GeneticsOctober 1, 2023Zhi-cheng Jia, Yong-qian Li, Bo-wen Zhou, Qing-chang Xia, Pei-xuan Wang, Xiao-xuan Wang, Zhen-gao Sun, Ying GuoU-labeled second-stranded DNAs with E. coli DNA polymerase I (NEB, cat.m0209, USA), RNase H (NEB, cat.m0297, USA), and dUTP solution (Thermo Fisher, cat.R0133, USA). A-bases were added to the blunt ends of each

(19) Changes in m(6)A RNA methylation are associated with male sterility in wolfberryBMC plant biologySeptember 29, 2023Zhao J, Zhang C, Li S, Yuan M, Mu W, Yang J, Ma Y, Guan C, Ma C.second-stranded DNAs was synthesized with E.coli DNA polymerase I (NEB, cat.m0209, USA), RNase H (NEB, cat.m0297, USA) and the dUTP Solution (Thermo Fisher, cat.R0133, USA). Next, the A base, which connected to the

(20) Integrative Transcriptomics and Proteomics Analysis Reveals Immune Response Process in Bovine Viral Diarrhea Virus-1-Infected Peripheral Blood Mononuclear CellsVeterinary SciencesSeptember 28, 2023Kang Zhang, Jingyan Zhang, Lei Wang, Qiang Liang, Yuhui Niu, Linlin Gu, Yanming Wei, Jianxi Liemploying E. coli DNA polymerase I (cat. m0209; New England Biolabs, Ipswich, Ma, USA), RNase H (cat. m0297; New England Biolabs, Ipswich, MA, USA), and a solution containing dUTP (cat. R0133; Thermo Fisher Scientific,

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RNase H from New England Biolabs

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RNase H from New England Biolabs

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