Description
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
Description:
RNase H (Ribonuclease H ) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single or double-stranded DNA.
Source:
An
E. coli strain that carries the cloned RNase H gene (rnh) from
Escherichia coli Applications:
- Removal of poly(A) tails of mRNA hybridized to poly(dT)
- Removal of mRNA during second strand cDNA synthesis
Reagents Supplied:
RNase H Reaction Buffer (10X)