Description
KEY FEATURES AND BENEFITS
- Fast and easy processing using efficient spin-column procedure
- Total RNA, genomic DNA and proteins are all column-purified
using the same column
- Sequential isolation of nucleic acids and proteins from a single
lysate: no need to split the lysate
- Isolate high-quality RNA in all sizes, including large mRNA
and rRNA to microRNA (miRNA)
- Analysis is more reliable since RNA, DNA and proteins are
derived from the same sample
- Isolate high-quality genomic DNA with a molecular weight = 30kb
- High yields of isolated proteins
- No phenol or chloroform extractions
KIT SPECIFICATIONS:
Column Binding Capacity ...................................... 50μ g for RNA
20μ g for DNA
200μ g for protein
Maximum Column Loading Volume ................600μ L
Size of RNA Purified ................................................All sizes, including small RNA (<200 nt)
Size of DNA Purified ............................................... 30kb
Maximum Amount of Starting Material
Animal cells ............................................................. 3 x 106 cells
Animal tissues .......................................................25mg
Blood .............................................................................100μ L
Bacteria ....................................................................... 1 x 109 cells
Yeast .............................................................................. 1 x 108 cells
Fungi ..............................................................................50mg
Plant Tissues ...........................................................50mg
Time to complete purification process ......30 minutes for 10 purifications
Average Yields from RNA Purification
HeLa Cells (1 x 106 cells) .................................15μ g RNA
HeLa Cells (1 x 106 cells) .................................8μ g DNA
HeLa Cells (1 x 106 cells) .................................150μ g protein