10X Flexi Enzyme Blend (SgfI & PmeI) from Promega

Product Specs
Item10X Flexi Enzyme Blend (SgfI & PmeI)
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberR1851
Quantity25microl

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Flexi Vector CloningMethods in Molecular BiologyFebruary 9, 2018Paul G. Blommel, Peter A. Martin, Kory D. Seder, Russell L. Wrobel, Brian G. FoxDNA Institute. 4.2.3. Flexi Vector Reagents The SgfI/PmeI 10X Enzyme Blend and Buffer (Product No. R1852), high concentration T4 DNA ligase (M1794), Magnesil PCR cleanup kits (A923A), Magnebot II magnetic

(2) Involvement of Conserved Amino Acids in the C-Terminal Region of LINE-1 ORF2p in RetrotranspositionGeneticsMarch 1, 2017Claiborne M Christian, Mark Sokolowski, Dawn deHaro, Kristine J Kines, Victoria P BelancioORF2 sequence of interest. PCR products were digested with SgfI/PmeI blend (Promega; catalogue number R1852) and cloned into the pBind vector (Promega Checkmate Mammalian Two Hybrid System; catalog number C934A).

(3) The importance of L1 ORF2p cryptic sequence to ORF2p fragment-mediated cytotoxicityMobile Genetic ElementsJuly 3, 2016Claiborne M. Christian, Kristine J. Kines, Victoria P. BelancioORF2 sequence of interest. PCR products were digested with SgfI/PmeI blend (Promega: catalog number R1852) and cloned into both the pAct and pBind vector (Promega Checkmate Mammalian Two Hybrid System: catalog

(4) Identification of L1 ORF2p sequence important to retrotransposition using Bipartile Alu retrotransposition (BAR)Nucleic Acids ResearchJune 2, 2016Claiborne M. Christian, Dawn deHaro, Kristine J. Kines, Mark Sokolowski, Victoria P. BelancioORF2 sequence of interest. PCR products were digested with SgfI/PmeI blend (Promega: catalogue number R1852) and cloned into the pBind vector (Promega Checkmate Mammalian Two Hybrid System: catalogue number C934A).

(5) MARCC (Matrix‐Assisted Reader Chromatin Capture): An Antibody‐Free Method to Enrich and Analyze Combinatorial Nucleosome ModificationsCurrent Protocols in Molecular BiologyJuly 1, 2015Zhangli Su, John M. Denu(GeneJet Gel Extraction Kit, Thermo Scientific, cat no. K0692) Restriction enzymes (10X Flexi Enzyme Blend, Promega, cat no. R1851) containing: 5X Flexi Digestion BufferFlexi Enzyme Blend (SgfI and PmeI)Carboxy Flexi Enzyme Blend (PmeI

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