Description
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
- Supplied with 2 µg control DNA
Description:
Cre Recombinase is a Type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between
loxP sites. The enzyme requires no energy cofactors and Cre-mediated recombination quickly reaches equilibrium between substrate and reaction products. The
loxP recognition element is a 34 base pair (bp) sequence comprised of two 13 bp inverted repeats flanking an 8 bp spacer region which confers directionality. Recombination products depend on the location and relative orientation of the
loxP sites. Two DNA species containing single
loxP sites will be fused. DNA between directly repeated
loxP sites will be excised in circular form while DNA between opposing
loxP sites will be inverted with respect to external sequences.
Source:
Purified from an
E. coli strain carrying a plasmid encoding Cre Recombinase from bacteriophage P1 with additional N-terminal Ala and Gly residues.
Applications:
- Excision of DNA between two loxP sites
- Fusion of DNA molecules containing loxP sites
- Inversion of DNA between loxP sites
Reagents Supplied:
pLox2+ (linearized)
Cre Recombinase Reaction Buffer (10X)