A revolutionary new approach to reagent deposition, the CHIP Chemical Printer allows accurate, reproducible, and reliable deposition of a wide variety of solutions to an even wider range of sample surfaces. The novel, patented combination of a high-resolution scanner with an array of four piezo-electric print heads makes accurate delivery of 100 picoliter droplets to the smallest features of sample/tissue surfaces possible. There is no longer reason to wonder if differences in ion signal are due to unintended variation in matrix or reagent deposition–the CHIP “digitizes” this process, introducing unsurpassed consistency to MALDI tissues analysis. Innovative software further simplifies complex profiling/imaging experiments by supporting point-file export to a range of MALDI-TOF mass spectrometers for fast and straightforward creation of automated experiments. In addition, each high-resolution sample image and the experimental features are archived for later reference. Application areas include: • Direct MS/MS analysis of proteins blotted on membrane • Direct identification of proteins detected by Western-blotting • New and emerging applications for printing MALDI matrices onto histological sections • Accelerating nanowestern applications The first step in the mass spectrometric investigation of surfaces is reproducibility and consistency–the first step is the CHIP.
Microscale on-membrane PMF analysis can be conducted on proteins transferred to a PVDF membrane utilizing the microdispensing function of a chemical printer (Refer to AXIMA Application 14, 17). Moreover, using the AXIMA-QIT ion trap MS in conjunction with the chemical printer enables MS analysis with high mass accuracy (<0.1Da). In this application, we succeeded in conducting MS/MS analysis of proteins immobilized on PVDF transfer membranes, something which has been difficult up to now due to the irregularities of the membrane surface and electrical charge. Direct on-membrane MS/MS analysis using the chemical printer together with the AXIMA-QIT, in addition to the established methodology for onmembrane PMF analysis, is expected to be extremely effective in identification of unknown proteins. Read More
Shimadzu Biotech, a global business unit of Shimadzu Corporation and Proteome Systems today announced that their jointly developed Chemical InkJet Printer (CHIP 1000) has been selected as one of the 100 most technologically significant products introduced into the marketplace over the past year. The R&D 100 award was bestowed by the independent judging panel and editors of R&D Magazine, in what was highlighted as a very competitive year of nominations. Read More