A microtiter plate was coated over night with Mouse anti Bovine interferon-γ antibody, clone CC330 (MCA2112) at concentration of 5μg/ml in ELISA coating buffer (BUF030A) and recombinant bovine interferon gamma (PBP007) as a standard. After washing with ELISA Wash buffer (BUF031A) and blocking with ELISA BSA Block (BUF032A) samples were detected using biotinylated Mouse anti Bovine interferon-γ antibody (MCA1783B) at 5μg/ml followed by incubation with streptavidin:HRP conjugate (STAR5B) at 1/1000. Finally, TMB core HRP substrate (BUF056A) was applied for color development. Data is presented as the mean of three measurements.
A microtiter plate was coated over night with MCA2112 at concentration of 5μg/ml in. After washing with and blocking with samples were detected using antibody at 5μg/ml followed by incubation with streptavidin:HRP conjugate at 1/1000. Finally was used as substrate. Data is presented as the mean of three measurements.
A microtiter plate was coated over night with MCA2112 at concentration of 5μg/ml in BUF030A. After washing with BUF031A and blocking with BUF032A samples were detected using MCA1783B antibody at 5μg/ml followed by incubation with streptavidin:HRP conjugate STAR5B at 1/1000. Finally, BUF056A was used as substrate. Data is presented as the mean of three measurements.
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