TMB ELISA PEROXIDASE SUBSTRATE from Rockland Immunochemicals, Inc.

Product Specs
ItemTMB ELISA PEROXIDASE SUBSTRATE
CompanyRockland Immunochemicals, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberTMBE-1000
Quantity1 L
Conjugate/Tag/LabelUnconjugated
PurityTMB ELISA Peroxidase Substrate specifications: pH: 4.0 +/- 0.2 Stability @ 18° to 26°C: PASS Stability @ 4°C: PASS QC Raw Material: PASS Absorbance check of final product: PASS Performance Data per ELISA: PASS
ApplicationsELISA
FormatLiquid - clear to very light blue colored liquid
Concentration1X
StorageStore container at 4° C prior to opening. Protect from moisture and light. No special shipping conditions or precautions are required

Specifications/Features

Application Note: TMB ELISA Peroxidase Substrate comes ready to use. No dilutions are required. TMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions.

Add to Compare List

Rockland Immunochemicals, Inc.

P.O. Box 5199

Limerick, Pennsylvania 19468

United States

Phone: (484) 791-3823
Fax: (484) 369-8654

Company Profile

Website: https://www.rockland.com

(1) AMPK suppresses Th2 cell responses by repressing mTORC2Experimental & molecular medicineAugust 1, 2022Pandit M, Timilshina M, Gu Y, Acharya S, Chung Y, Seo SU, Chang JH.dilution of 1:1000. Subsequently, the plates were incubated with TMBE ELISA peroxidase substrate (Cat # TMBE-1000; Rockland Immunochemicals, Inc., PA, USA) for 15 min in the dark followed by 2 N H2SO4. The absorbance

(2) Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISAJournal of immunological methodsJune 1, 2022Quach HQ, Ovsyannikova IG, Poland GA, Kennedy RB.(catalog no. 207–4302) and TMB (3, 3′, 5, 5’-Tetramethylbezidine) ELISA peroxidase substrate (catalog no. TMBE-1000) were purchased from Rockland Immunochemicals, Inc. (Limerick, PA). Bovine serum albumin (BSA, catalog

(3) LKB1-PTEN axis controls Th1 and Th17 cell differentiation via regulating mTORC1Journal of molecular medicine (Berlin, Germany)August 1, 2021Pandit M, Timilshina M, Chang JH.

(4) Immunological imprint of COVID-19 on human peripheral blood leukocyte populationsAllergyMarch 1, 2021Kratzer B, Trapin D, Ettel P, Körmöczi U, Rottal A, Tuppy F, Feichter M, Gattinger P, Borochova K, Dorofeeva Y, et al.

(5) Serum lipoprotein-derived fatty acids regulate hypoxia-inducible factorThe Journal of biological chemistryDecember 25, 2020Shao W, Hwang J, Liu C, Mukhopadhyay D, Zhao S, Shen MC, Selen ES, Wolfgang MJ, Farber SA, Espenshade PJ.

(6) Convergent structural features of respiratory syncytial virus neutralizing antibodies and plasticity of the site V epitope on prefusion FPLoS pathogensNovember 1, 2020Harshbarger W, Tian S, Wahome N, Balsaraf A, Bhattacharya D, Jiang D, Pandey R, Tungare K, Friedrich K, Mehzabeen N, et al.at RT, followed by a wash and incubation for 20 min with 100 μl/well of TMB substrate (Rockland cat# TMBE-1000) at RT. Following incubation, the reaction was stopped by adding 100 μl/well of 2.0 N Sulfuric Acid

(7) Assessing the compatibility of primary human hepatocyte culture within porous silk spongesRSC advancesOctober 12, 2020Kukla DA, Stoppel WL, Kaplan DL, Khetani SR.

(8) Antibody-secreting cell destiny emerges during the initial stages of B-cell activationNature communicationsAugust 10, 2020Scharer CD, Patterson DG, Mi T, Price MJ, Hicks SL, Boss JM.Biotechnology; 1021-05), washed, and incubated with tetramethylbenzidine ELISA peroxidase substrate (Rockland; TMBE-1000) for 20 min at room temperature (50 μl/well). 50 μl of 0.2 M sulfuric acid was applied to each well

(9) Microscale Collagen and Fibroblast Interactions Enhance Primary Human Hepatocyte Functions in Three-Dimensional ModelsGene expressionJune 12, 2020Kukla DA, Crampton AL, Wood DK, Khetani SR.

(10) The Histone Demethylase LSD1 Regulates B Cell Proliferation and Plasmablast DifferentiationJournal of immunology (Baltimore, Md. : 1950)November 1, 2018Haines RR, Barwick BG, Scharer CD, Majumder P, Randall TD, Boss JM.at room temperature, and washed. Plates were incubated with TMB ELISA peroxidase substrate (Rockland TMBE-1000) for twenty minutes at room temperature (50 μl/well) to develop a color reaction. 50 μl of 0.2 M sulfuric

(11) Intra-seasonal antibody repertoire analysis of a subject immunized with an MF59®-adjuvanted pandemic 2009 H1N1 vaccineVaccineAugust 23, 2018Ferdman J, Palladino G, Liao HX, Moody MA, Kepler TB, Del Giudice G, Dormitzer PR, Harrison SC, Settembre EC, Suphaphiphat P.incubated 1h at RT washed three times. Colorimetric outcomes were developed with TMB Substrate (Rockland, TMBE-1000) for 5 min at RT and stopped with 2N sulfuric acid (BDH, BDH3500–1). Plates were incubated an additional

(12) Antibodies against adenovirus fiber and penton base proteins inhibit adenovirus vector-mediated transduction in the liver following systemic administrationScientific reportsAugust 17, 2018Tomita K, Sakurai F, Iizuka S, Hemmi M, Wakabayashi K, Machitani M, Tachibana M, Katayama K, Kamada H, Mizuguchi H.

(13) B cell activation and plasma cell differentiation are inhibited by de novo DNA methylationNature communicationsMay 15, 2018Barwick BG, Scharer CD, Martinez RJ, Price MJ, Wein AN, Haines RR, Bally APR, Kohlmeier JE, Boss JM.

(14) Pre-fusion RSV F strongly boosts pre-fusion specific neutralizing responses in cattle pre-exposed to bovine RSVNature communicationsOctober 20, 2017Steff AM, Monroe J, Friedrich K, Chandramouli S, Nguyen TL, Tian S, Vandepaer S, Toussaint JF, Carfi A.at RT, followed by a wash and incubation for 20 min with 100 μl/well of TMB substrate (Rockland cat# TMBE-1000) at RT. Following incubation, the reaction was stopped by adding 100 μl/well of 2.0 N Sulfuric Acid

(15) Immunometric Antibody Sandwich Enzyme-Linked Immunosorbent AssayCold Spring Harbor ProtocolsJune 1, 2017Thomas O. Kohl, Carl A. Ascoliat http://cshprotocols.cshlp.org/site/recipes. Reagents 3,3′ ,5,5′ -tetramethylbenzidine (TMB) (Rockland Immunochemicals, #TMBE-1000) (for horseradish peroxidase [HRP]-based assays) Antibodies of known concentration for antigen-speciﬁc

(16) Immunometric Double-Antibody Sandwich Enzyme-Linked Immunosorbent AssayCold Spring Harbor ProtocolsJune 1, 2017Thomas O. Kohl, Carl A. Ascoliat http://cshprotocols.cshlp.org/site/recipes. Reagents 3,3′ ,5,5′ -tetramethylbenzidine (TMB) (Rockland Immunochemicals, #TMBE-1000) (for horseradish peroxidase [HRP]-based assays) Antibody diluent solution (1% [v/v] ﬁsh gel [Sigma-Aldrich,

(17) Indirect Immunometric ELISACold Spring Harbor ProtocolsMay 3, 2017Thomas O. Kohl, Carl A. Ascoliat http://cshprotocols.cshlp.org/site/recipes. Reagents 3,3′ ,5,5′ -tetramethylbenzidine (TMB) (Rockland Immunochemicals, #TMBE-1000) (for HRP-based assays) Antibodies of known concentration Antibody diluent solution (1% [v/v] ﬁsh gel

(18) Novel antibody probes for the characterization of endosialin/TEM-1OncotargetOctober 25, 2016O'Shannessy DJ, Smith MF, Somers EB, Jackson SM, Albone E, Tomkowicz B, Cheng X, Park Y, Fernando D, Milinichik A, et al.

(19) Cytomegalovirus viral load within blood increases markedly in healthy people over the age of 70 yearsImmunity & ageing : I & AJanuary 1, 2016Parry HM, Zuo J, Frumento G, Mirajkar N, Inman C, Edwards E, Griffiths M, Pratt G, Moss P.

(20) Antigenic Characterization of the HCMV gH/gL/gO and Pentamer Cell Entry Complexes Reveals Binding Sites for Potently Neutralizing Human AntibodiesPLoS pathogensOctober 1, 2015Ciferri C, Chandramouli S, Leitner A, Donnarumma D, Cianfrocco MA, Gerrein R, Friedrich K, Aggarwal Y, Palladino G, Aebersold R, et al.at RT. Wells were washed and incubated for 30 min with 100 μl/well of TMB substrate (Rockland cat# TMBE-1000). Following incubation, the reaction was stopped by adding 100 μl/well of 2.0 N Sulfuric Acid (BDH cat#

Be the First to Write a Review!

TMBE ELISA Peroxidase Substrate - ELISA