TMB High Sensitivity Substrate Solution from BioLegend

Product Specs
ItemTMB High Sensitivity Substrate Solution
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number421501
ApplicationsELISA
Quantity60 mL
TypeSOLUTION

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Short Carbon Nanotube-Based Delivery of mRNA for HIV-1 VaccinesBiomoleculesJuly 7, 2023Yang Xu, Tammy Ferguson, Kazuya Masuda, Mohammad Adnan Siddiqui, Kelsi Poole Smith, Olivia Vest, Brad Brooks, Ziyou Zhou, Judy Obliosca, Xiang-Peng Kong, et al.PBS-0.05% Tween and 3 times with PBS, 75 µL/well of TMB High Sensitivity Substrate at RT (BioLegend Cat#: 421501, San Diego, CA, USA) was added and the plates were left for 10 to 15 min in the dark. Briefly, 75 µL/well

(2) Primary and secondary functions of HLA-E are determined by stability and conformation of the peptide-bound complexesCell ReportsJune 14, 2022Lucy C. Walters, Daniel Rozbesky, Karl Harlos, Max Quastel, Hong Sun, Sebastian Springer, Robert P. Rambo, Fiyaz Mohammed, E. Yvonne Jones, Andrew J. McMichael, et al.Netherlands",https://ccb.lumc.nl/ ELISA Coating buffer(x5),Biolegend,Cat#421701 ELISA Wash Buffer (x20),Biolegend,Cat#421501 TMB High sensitivity Substrate,Biolegend,Cat#421501 Stop Solution,Biolegend,Cat#423001 ,, Critical

(3) Hepatocyte-specific activity of TSC22D4 triggers progressive NAFLD by impairing mitochondrial functionMolecular MetabolismJune 1, 2022Gretchen Wolff et al.accuracy was assessed using a colorimetric method with tetramethyl benzidine substrate (TMB, BioLegend, #421501) and Horseradish Peroxidase (HRP, Life Technologies, #31490) as described at Rodriguez et al. [24].

(4) Single-nucleus RNA-seq2 reveals functional crosstalk between liver zonation and ploidyNature CommunicationsDecember 1, 2021M. L. Richter et al.well-plates was assessed using a colorimetric method with tetramethyl benzidine substrate (TMB, BioLegend, #421501) and 50 μg/mL of Horseradish Peroxidase (HRP, Life Technologies, #31490)125. Only when the plate alignment

(5) Comparison of immunoglobulin E anti-thyroid peroxidase antibodies in patients with Hashimoto thyroiditis and chronic spontaneous urticariaMedicine and Pharmacy ReportsJanuary 29, 2021Songül Çildağ, Çiğdem Yenisey, Mustafa Ünübol, Taşkın Şentürkwere washed three times with washing buffer. Subsequently, 100 µL TMB substrate (Biolegend, Cat No. 421501, CA, USA) was added to each well and the plates were incubated at least 1 hour at 37°C (depending on

(6) Convalescent plasma-mediated resolution of COVID-19 in a patient with humoral immunodeficiencyCell Reports MedicineJanuary 19, 2021Kazuhito Honjo, Ronnie M. Russell, Ran Li, Weimin Liu, Regina Stoltz, Edlue M. Tabengwa, Yutao Hua, Lynn Prichard, Ashton N. Kornbrust, Sarah Sterrett, et al.Liquid Substrate",Sigma-Aldrich,Cat# T0440-1L "3,3′,5,5′-Tetramethylbenzidine (TMB)",BioLegend,Cat# 421501 Sulfuric Acid (1N),ICCA,Cat# 8300-32 SARS CoV2 His-tag,"Brouwer et al., Produced In House",PMID: 32540902 SARS

(7) Rapid, direct detection of bacterial topoisomerase 1-DNA adducts by RADAR/ELISAAnalytical BiochemistryNovember 1, 2020Devapriya Sinha, Kostantin Kiianitsa, David R. Sherman, Nancy Maizelsand washed as above. Signal detection was performed using TMB High Sensitivity Solution (BioLegend, #421501) according to manufacturer’s instructions. Absorbance was read at 450 and 570 nm, and the A570 reading

(8) Interrogating the recognition landscape of a conserved HIV-specific TCR reveals distinct bacterial peptide cross-reactivityeLifeJuly 27, 2020Mendoza JL, Fischer S, Gee MH, Lam LH, Brackenridge S, Powrie FM, Birnbaum M, McMichael AJ, Garcia KC, Gillespie GM.UV-peptide exchange ELISA (1:1000) "Chemical compound, drug",TMB High sensitivity Substrate,Biolegend,421501,Assay development step (2) for UV-peptide exchange ELISA (100 μL/well) "Chemical compound, drug",Stop

(9) Long-Term Delivery of an Anti-SIV Monoclonal Antibody With AAVFrontiers in ImmunologyMarch 17, 2020José M. Martinez-Navio, Sebastian P. Fuchs, Desiree E. Mendes, Eva G. Rakasz, Guangping Gao, Jeffrey D. Lifson, Ronald C. Desrosiers(Catalog# 2015-05, Santa Cruz) and developed with TMB High Sensitivity Substrate Solution (Catalog# 421501, Biolegend). The reaction was stopped by the addition of 50 µl of acidic stop solution (SouthernBiotech, Birmingham,

(10) Removal of N-Linked Glycosylation Enhances PD-L1 Detection and Predicts Anti-PD-1/PD-L1 Therapeutic EfficacyCancer cellAugust 12, 2019Lee HH, Wang YN, Xia W, Chen CH, Rau KM, Ye L, Wei Y, Chou CK, Wang SC, Yan M, et al.TMB Substrate,Cell Signaling Technology,Cat#7004 TMB High Sensitivity Substrate Solution,BioLegend,Cat#421501 STOP Solution,Cell Signaling Technology,Cat#7002 Stop Solution for TMB Substrate,BioLegend,Cat#423001 BSA

(11) Mass Spectral Detection of Diethoxyphospho-Tyrosine Adducts on Proteins from HEK293 Cells Using Monoclonal Antibody depY for EnrichmentChemical Research in ToxicologyJune 18, 2018Seda Onder, Lawrence M. Schopfer, Ozden Tacal, Thomas A. Blake, Rudolph C. Johnson, Oksana Lockridgeperoxidase was from Cell Signaling 7076, stored at −20 °C. 3,3′,5,5′-Tetramethylbenzidine was from BioLegend 421501. Trifluoroacetic acid sequencing grade was from Beckman 290204. Chlorpyrifos oxon 98% pure was from

(12) Designing and overproducing a tandem epitope of gp350/220 that shows a potential to become an EBV vaccineHeliyonMarch 1, 2018Widodo, Veronica Margarecaesha Anyndita N, Dluha N, Rifa'i M, Himmah K, Wahyuningsih MD.After washing three separate times, 100 ul TMB High Sensitivity Substrate Solution (Catalog number: 421501, BioLegend) was added to wells for 2 min, followed by the addition of 50 ul of stop solution. The absorbance

(13) Interplay of PA-X and NS1 Proteins in Replication and Pathogenesis of a Temperature-Sensitive 2009 Pandemic H1N1 Influenza A VirusJournal of virologySeptember 1, 2017Nogales A, Rodriguez L, DeDiego ML, Topham DJ, Martínez-Sobrido L.

(14) The immunoreceptor NKG2D promotes tumour growth in a model of hepatocellular carcinomaNature communicationsJanuary 27, 2017Sheppard S, Guedes J, Mroz A, Zavitsanou AM, Kudo H, Rothery SM, Angelopoulos P, Goldin R, Guerra N.

(15) Acetylated Histones in Apoptotic Microparticles Drive the Formation of Neutrophil Extracellular Traps in Active Lupus NephritisFrontiers in immunologyJanuary 1, 2017Rother N, Pieterse E, Lubbers J, Hilbrands L, van der Vlag J.

(16) Determinants of VH1-46 Cross-Reactivity to Pemphigus Vulgaris Autoantigen Desmoglein 3 and Rotavirus Antigen VP6Journal of immunology (Baltimore, Md. : 1950)August 15, 2016Cho MJ, Ellebrecht CT, Hammers CM, Mukherjee EM, Sapparapu G, Boudreaux CE, McDonald SM, Crowe JE Jr, Payne AS.

(17) Influenza A virus attenuation by codon deoptimization of the NS gene for vaccine developmentJournal of virologySeptember 1, 2014Nogales A, Baker SF, Ortiz-Riaño E, Dewhurst S, Topham DJ, Martínez-Sobrido L.

(18) Ultrasensitive isolation, identification and quantification of DNA-protein adducts by ELISA-based RADAR assayNucleic acids researchJuly 1, 2014Kiianitsa K, Maizels N.

(19) Neurofascin IgG4 antibodies in CIDP associate with disabling tremor and poor response to IVIgNeurologyMarch 11, 2014Querol L, Nogales-Gadea G, Rojas-Garcia R, Diaz-Manera J, Pardo J, Ortega-Moreno A, Sedano MJ, Gallardo E, Berciano J, Blesa R, et al.

(20) Characterization of an arachidonic acid-deficient (Fads1 knockout) mouse modelJournal of lipid researchJuly 1, 2012Fan YY, Monk JM, Hou TY, Callway E, Vincent L, Weeks B, Yang P, Chapkin RS.

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TMB High Sensitivity Substrate Solution from BioLegend

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TMB High Sensitivity Substrate Solution from BioLegend

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