Clarity™ Western ECL Substrate, 200 ml from Bio-Rad

Product Specs
ItemClarity™ Western ECL Substrate, 200 ml
CompanyBio-Rad
Price Supplier Page View Company Product Page
Catalog Number1705060
Quantity200 mL

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

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Website: http://discover.bio-rad.com

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(1) Splice-Switching Antisense Oligonucleotides Targeting Extra- and Intracellular Domains of Epidermal Growth Factor Receptor in Cancer CellsBiomedicinesDecember 13, 2023Akilandeswari Ashwini Balachandran, Prithi Raguraman, Kamal Rahimizadeh, Rakesh N. Veedufor two hours at room temperature. After washing, a Clarity Western ECL detection kit (Biorad; Cat# 1705060, South Granville, NSW, Australia) was used to detect the signals. Images were captured using the Fusion

(2) MicroRNA-451a inhibits gemcitabine-refractory biliary tract cancer progression by suppressing the MIF-mediated PI3K/AKT pathwayMolecular Therapy - Nucleic AcidsDecember 12, 2023Taisuke Obata, Koichiro Tsutsumi, Eijiro Ueta, Takashi Oda, Tatsuya Kikuchi, Soichiro Ako, Yuki Fujii, Tatsuhiro Yamazaki, Daisuke Uchida, Kazuyuki Matsumoto, et al.1:2,000 and 1:4,000, respectively. Reactive bands were detected using Clarity Western ECL Substrate (#1705060; Bio-Rad) and ImageQuant LAS 4000 (GE Healthcare Bio-Sciences AB, Uppsala, Sweden). The signal intensity

(3) Ubiquitin signaling and the proteasome drive human DNA–protein crosslink repairNucleic Acids ResearchDecember 11, 2023Maram Essawy, Lisa Chesner, Duha Alshareef, Shaofei Ji, Natalia Tretyakova, Colin Campbellused at a 1:10 000 dilution. Clarity Max ECL Western Blotting Substrate was purchased from Bio-Rad (#1705060). Biological resources Human oxoguanine glycosylase 1 (OGG1) was expressed and purified from BL21(DE3)

(4) Effects of 1,25-Vitamin D3 and 24,25-Vitamin D3 on Corneal Nerve Regeneration in Diabetic MiceBiomoleculesDecember 6, 2023Xiaowen Lu, Zhong Chen, Jerry Lu, Mitchell A. Watskythe enhanced chemiluminescence (ECL) detection system were obtained from Bio-Rad (Catalog # 1704272, 1705060). 2.2. Animal Experiments All animal studies were approved by the Augusta University IACUC, and the

(5) Systematic assessment of transcriptomic and metabolic reprogramming by blue light exposure coupled with agingPNAS NexusDecember 5, 2023Jia Huang, Fan Zhou, Huanchan Zhou, Xiaoqi Zheng, Zhengyi Huo, Meng Yang, Zihe Xu, Runzhou Liu, Luoluo Wang, Xiaoyun Wangcorresponding secondary antibody, each targeted protein was detected using Clarity Western ECL Substrate (#1705060, Bio-Rad, Hercules, CA, USA) and photographed with a Tanon 5200 Chemiluminescent Imaging System (Tanon,

(6) The role of inflammation and antioxidant defenses in the cardiotoxicity of doxorubicin in elderly CD-1 male miceArchives of ToxicologyDecember 1, 2023Ana Reis-Mendes, Mariana Ferreira, José Alberto Duarte, Margarida Duarte-Araújo, Fernando Remião, Félix Carvalho, Emília Sousa, Maria Lourdes Bastos, Vera Marisa Costaand immunohistochemistry are shown in Supplementary Table S1. Enhanced chemiluminescence (Clarity Western ECL, ref. 1705060) reagents and the Bio-Rad DC protein assay kit were bought from Bio-Rad Laboratories (Hercules, CA,

(7) DYRK-family kinases regulate Candida albicans morphogenesis and virulence through the Ras1/PKA pathwaymBioNovember 28, 2023Jessie MacAlpine, Zhongle Liu, Saif Hossain, Luke Whitesell, Nicole Robbins, Leah E. Cowenfor 10 minutes each, the blot was developed using the Clarity Western ECL Substrate Kit (Bio-Rad, #1705060), and the blot was imaged on a ChemiDoc Bio-Rad imager. Mouse model of C. albicans dermatitis BALB/c

(8) Biophysical properties of NaV1.5 channels from atrial-like and ventricular-like cardiomyocytes derived from human induced pluripotent stem cellsScientific ReportsNovember 24, 2023Charles-Albert Chapotte-Baldacci, Marion Pierre, Mohammed Djemai, Valérie Pouliot, Mohamed Chahinesecondary antibodies. Proteins were revealed using Clarity and Clarity Max Western ECL substrates(Cat# 1705060 and Cat# 1705062, Bio-Rad) and were visualized using the ChemiDoc system (Bio-Rad, ON, Canada). Relative

(9) Neither alpha-synuclein-preformed fibrils derived from patients with GBA1 mutations nor the host murine genotype significantly influence seeding efficacy in the mouse olfactory bulbbioRxivNovember 23, 2023Sara Walton, Alexis Fenyi, Tyler Tittle, Ellen Sidransky, Gian Pal, Solji Choi, Ronald Melki, Bryan A. Killinger, Jeffrey H. KordowerNaCl, 0.1% Tween-20) and imaged using enhanced chemiluminescence (ECL) substrate (Biorad, product # 1705060) and Chemidoc imager (Biorad). Western Blotting Proteins were extracted from fixed floating tissue

(10) Flavones provide resistance to DUX4-induced toxicity via an mTor-independent mechanismCell Death & DiseaseNovember 16, 2023Justin Cohen, Shushu Huang, Katherine E. Koczwara, Kristen T. Woods, Vincent Ho, Keryn G. Woodman, Jack L. Arbiser, Katelyn Daman, Monkol Lek, Charles P. Emerson, et al.(Cell Signaling 7074P2 and 7076S). ECL reagents: Clarity Western ECL Substrate (BIO-RAD, Hercules, CA 1705060) and Clarity MAX Western ECL Substrate (BIO-RAD1705062). Blots were visualized on a BIO-RAD ChemiDoc

(11) The Novel Tetra-Specific Drug C-192, Conjugated Using UniStac, Alleviates Non-Alcoholic Steatohepatitis in an MCD Diet-Induced Mouse ModelPharmaceuticals (Basel)November 13, 2023Jihye Kim, Nakho Chang, Yunki Kim, Jaehyun Lee, Daeseok Oh, Jaeyoung Choi, Onyou Kim, Sujin Kim, Myongho Choi, Junyeob Lee, et al.from TOCRIS (Bristol, UK). The protein bands were visualized using Clarity™ Western ECL Substrate (#1705060, Biorad, Hercules, CA, USA) after hybridization with 1:5000 diluted HRP-conjugated secondary antibodies

(12) The Ultraviolet Irradiation of Keratinocytes Induces Ectopic Expression of LINE-1 Retrotransposon Machinery and Leads to Cellular SenescenceBiomedicinesNovember 10, 2023Fadi Touma, Marine Lambert, Amelia Martínez Villarreal, Jennifer Gantchev, Brandon Ramchatesingh, Ivan V. LitvinovRRID: AB_2107311), 1:2000 dilution in 1% BSA in PBS, and Clarity™ Western ECL Substrate (BioRad Cat# 1705060, Hercules, CA, USA). 2.4. RT-qPCR For the mRNA analysis, 100,000 HaCaT or 300,000 N/TERT1 cells were

(13) Sex differences in the neuronal transcriptome and synaptic mitochondrial function in the cerebral cortex of a multiple sclerosis modelFrontiers in NeurologyNovember 2, 2023Noriko Itoh, Yuichiro Itoh, Linsey Stiles, Rhonda Voskuhlmin in 1xPBST. Detection was achieved using Clarity™ Western ECL Substrate (BioRad, Hercules, CA, #1705060) and imaged by Azure c300 (Azure Biosystems, Dublin, CA). The following primary antibodies were used:

(14) Voltage-dependent anion channel 1 (VDAC1) overexpression alleviates cardiac fibroblast activation in cardiac fibrosis via regulating fatty acid metabolismRedox BiologyNovember 1, 2023Geer Tian, Junteng Zhou, Yue Quan, Qihang Kong, Junli Li, Yanguo Xin, Wenchao Wu, Xiaoqiang Tang, Xiaojing Liuprimary and secondary antibodies were detected using an enhanced chemiluminescence (ECL) kit (Bio-Rad, 1705060). Immunoblots were quantified using Image J software. The primary antibodies used in this study were

(15) Early Depletion of Neutrophils Reduces Retinal Inflammation and Neovascularization in Mice with Oxygen-Induced RetinopathyInternational Journal of Molecular SciencesOctober 27, 2023Devy Deliyanti, Varaporn Suphapimol, Phoebe Ang, Xiuying Tang, Abhirup Jayasimhan, Jennifer L. Wilkinson-Berkafor 1 h at room temperature. Protein bands were visualized using the ECL detection system (Bio-Rad, #1705060) and a ChemiDoc XRS+ (BioRad). Signals were quantified using ImageJ software v1.5.3 (National Institute

(16) The GPCR adaptor protein Norbin regulates S1PR1 trafficking and the morphology, cell cycle and survival of PC12 cellsScientific ReportsOctober 25, 2023Valdemar B. I. Johansen, Elizabeth Hampson, Elpida Tsonou, Chiara Pantarelli, Julia Y. Chu, Laraine Crossland, Hanneke Okkenhaug, Andrew J. Massey, David C. Hornigold, Heidi C. E. Welch, et al.goat anti-rabbit IgG (Bio-Rad, 1706515, 1:3000). Detection was done using Clarity ECL reagent (Bio-Rad, 1705060, 1:3000). Blots for phosphorylated proteins were done first, then stripped, and reprobed for the total

(17) MED19 encodes two unique protein isoforms that confer prostate cancer growth under low androgen through distinct gene expression programsScientific ReportsOctober 25, 2023Rachel Ruoff, Hannah Weber, Ying Wang, Hongying Huang, Ellen Shapiro, David Fenyö, Michael J. Garabedianloading control. Protein bands were visualized using a Clarity Western ECL Substrate (BioRad; cat #1705060), and images were acquired on an iBright CL1000 (ThermoFisher Scientific) using the “Auto-Exposure”

(18) Combination of Cysteine and Glutathione Prevents Ethanol-Induced Hangover and Liver Damage by Modulation of Nrf2 Signaling in HepG2 Cells and MiceAntioxidants (Basel)October 20, 2023Hyeongyeong Kim, Hyung Joo Suh, Ki-Bae Hong, Eun-Jin Jung, Yejin Ahnreacted at 25 °C for 2 h. Next, the membrane was incubated with electrochemiluminescence substrate (#1705060, Bio-Rad, Hercules, CA, USA) for 5 min and analyzed using FluorChem M (ProteinSimple, Santa Clara, CA,

(19) Cryptococcus neoformans adapts to the host environment through TOR-mediated remodeling of phospholipid asymmetryNature CommunicationsOctober 18, 2023Laura C. Ristow, Andrew J. Jezewski, Benjamin J. Chadwick, Mark A. Stamnes, Xiaorong Lin, Damian J. Krysanwas washed 3 times for 5 min with TBST, then developed with chemiluminescent substrate (catalog no. 1705060; BioRad) and imaged on a myECL imager (ThermoFisher). Rim101-GFP pulldown and western blot GFP-pulldown

(20) Synergistic roles of CBX4 chromo and SIM domains in regulating senescence of primary human osteoarthritic chondrocytesArthritis Research & TherapyOctober 12, 2023Yu-Hsiu Chen, Xin Zhang, David Attarian, Virginia Byers Kraustemperature for 1 h. β-actin protein bands were visualized using Clarity™ Western ECL Substrate (Bio-Rad, 1705060); CBX4 protein bands were visualized using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo

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(1)

4/13/2020Sensitive and Stable ECL Substrate from Bio-Rad Elva ZhangDefinitely recommended for western blot experiments.
(1)

8/20/2015Clarity Western Blotting ECL Substrate Evan HermelWe have had difficulties getting good signal on our Licor digital western blot imager, using standard ECL substrates. Bio-Rad's Clarity reagent solved this problem with a good signal after a shorter ... Read Review

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