Nucleus Pulposus Cell Medium from ScienCell Research Laboratories

Product Specs
ItemNucleus Pulposus Cell Medium
CompanyScienCell Research Laboratories
Price Pricing Info Supplier Page View Company Product Page
Catalog Number4801
Quantity500 ml
TypeMedia

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ScienCell Research Laboratories

1610 Faraday Ave

Carlsbad, California 92008

United States

Phone: 877-602-8549
760-602-8575

Email: info@ScienCellonline.com

Company Profile

Website: http://www.sciencellonline.com

(1) Inhibition of miR-96-5p alleviates intervertebral disc degeneration by regulating the peroxisome proliferator-activated receptor γ/nuclear factor-kappaB pathwayJ Orthop Surg ResDecember 1, 2023Xusheng Li, Qian Hou, Wenqi Yuan, Xuehua Zhan, Haifeng YuanScienCell Research Laboratories. Cells were cultured in the Nucleus Pulposus Cell Medium (NPCM, Cat. #4801) in an incubator at 37 °C and 5% CO2. In order to find the optimal experimental concentration, IL-1β

(2) Injectable decellularized nucleus pulposus tissue exhibits neuroinhibitory propertiesJOR SPINEMarch 1, 2022Logan M. Piening, David J. Lillyman, Fei San Lee, Alvaro Moreno Lozano, Jeremy R. Miles, Rebecca A. Wachschange. Briefly, 100 mg of wet decellularized NP tissue was eluted in 1 ml complete NP media (Sciencell, 4801) for 72 hours at 37°C. Human NP cells (Sciencell, 4800) were grown to passage 4, then split and seeded

(3) High hydrostatic pressure (30 atm) enhances the apoptosis and inhibits the proteoglycan synthesis and extracellular matrix level of human nucleus pulposus cells via promoting the Wnt/β-catenin pathwayBioengineeredFebruary 1, 2022Zongting Shi, Jun He, Jian He, Yuan Xu(Shanghai, China) was used to culture cells. Then, HNPCs were cultured in Nucleus Pulposus Cell Medium (4801, ScienCell Research Laboratories, Carlsbad, California, USA) at 37°C with 5% CO2, and the cell medium

(4) Circ_0004354 might compete with circ_0040039 to induce NPCs death and inflammatory response by targeting miR-345-3p-FAF1/TP73 axis in intervertebral disc degenerationOxidative medicine and cellular longevityJanuary 1, 2022Li Y, Wu X, Li J, Du L, Wang X, Cao J, Li H, Huo Z, Li G, Pan D, et al.Research Laboratories (Sciencell, Cat. #4800, USA). NPCs were cultured in NPC Medium (Sciencell, Cat. #4801, USA) containing 5 mL NPCs growth supplement, 5 mL penicillin/streptomycin solution, and 10 mL fetal

(5) Silencing ATF3 Might Delay TBHP-Induced Intervertebral Disc Degeneration by Repressing NPC Ferroptosis, Apoptosis, and ECM DegradationOxidative medicine and cellular longevityJanuary 1, 2022Li Y, Pan D, Wang X, Huo Z, Wu X, Li J, Cao J, Xu H, Du L, Xu B.(ScienCell, Cat. #4800, USA). The cells were cultured in Nucleus Pulposus Cell Medium (NPCM, Cat. #4801, ScienCell, USA) supplemented with 10 mL fetal bovine serum, 5 mL NPC growth supplement, and 5 mL penicillin/streptomycin

(6) Identification of circ-FAM169A sponges miR-583 involved in the regulation of intervertebral disc degenerationJournal of Orthopaedic TranslationJanuary 1, 2021Yongjin Li, Dayu Pan, Shen Liu, Xuewu Xing, Hengxing Zhou, Bin Zhang, Di Zhang, Bo Li, Guowang Li, Bo Tao, et al.human intervertebral disc. NPCs were cultured in 500 ml Nucleus Pulposus Cell Medium (NPCM, Cat. #4801, Sciencell, USA) containing 10 ml fetal bovine serum, 5 ml NPCs growth supplement, and 5 ml penicillin/streptomycin

(7) Circ_0040039 May Aggravate Intervertebral Disk Degeneration by Regulating the MiR-874-3p-ESR1 PathwayFrontiers in geneticsJanuary 1, 2021Li Y, Wang X, Xu H, Li G, Huo Z, Du L, Zhang K, Shen L, Li H, Xu B.from the NP of human intervertebral disc. NPCs were cultured in Nucleus Pulposus Cell Medium (Cat. #4801, ScienCell, United States) containing 10 mL fetal bovine serum, 5 mL NPC growth supplement, and 5 mL

(8) Recombinant human bone morphogenetic protein 2 and 7 inhibit the degeneration of intervertebral discs by blocking the Puma-dependent apoptotic signalingInternational journal of biological sciencesJanuary 1, 2021Xie S, Zhao C, Chen W, Li G, Xiong Z, Tang X, Zhang F, Xiao H.ScienCell Research Laboratories (Carisbad, CA, USA; #4800). Cells were cultured in a NPCM (NP cell medium) (Carisbad; #4801) containing 10% fetal bovine serum (FBS) (Sigma-Aldrich, Shanghai, China; #F0926) and 100 U/mL penicillin-streptomycin

(9) Silencing of Long Non-coding RNA NEAT1 Upregulates miR-195a to Attenuate Intervertebral Disk Degeneration via the BAX/BAK PathwayFrontiers in Molecular BiosciencesAugust 11, 2020Ning Tang, Yulei Dong, Jiaming Liu, Hong ZhaoScienCell Research Laboratories (Carlsbad, CA, United States). The Nucleus Pulposus cell medium (Cat. No. 4801), consisting of 500 ml basal medium, 10 ml fetal bovine serum (FBS, Cat. No. 0010), 5 ml Nucleus Pulposus Cell Growth Supplement

(10) Resveratrol protects against apoptosis induced by interleukin-1β in nucleus pulposus cells via activating mTOR/caspase-3 and GSK-3β/caspase-3 pathwaysBioscience ReportsJuly 31, 2020Xiaohui Guo, Xiaoliang Bai, Feng Zhang, Long Zheng, Wenyuan Ding, Sidong Yangstudy Reagents/antibodies Manufacturers City/Country Catalog number Source DMEM/F12 Sciencell San Diego, CA, U.S.A. 4801 N/A FBS Sciencell San Diego, CA, U.S.A. 0025 Bovine Trypsin Gibco New York, U.S.A. 25200-056 Porcine DMSO MP California,

(11) Development of an in vitro intervertebral disc innervation model to screen neuroinhibitory biomaterialsJournal of Orthopaedic ResearchMay 1, 2020Sarah M. Romereim, Caleb A. Johnston, Adan L. Redwine, Rebecca A. WachsCell Culture Commercially available human NP cells (4800; ScienCell, Carlsbad, CA) were expanded in complete NP media (4801; ScienCell) in a T75 flask coated with poly‐L‐lysine (413; Scien-Cell) at 37°C in hypoxic conditions

(12) Gamma-secretase inhibitor suppressed Notch1 intracellular domain combination with p65 and resulted in the inhibition of the NF-κB signaling pathway induced by IL-1β and TNF-α in nucleus pulposus cellsJournal of cellular biochemistryFebruary 1, 2019Huang Y, Mei W, Chen J, Jiang T, Zhou Z, Yin G, Fan J.the volume fraction. 2.5 Cell culture hNPCs (ScienCell 4800, Carlsbad, CA) and cell‐specific media (4801) were purchased from ScienCell. hNPCs were cultured at 37°C/5% CO2, and then grown to 90% confluence.

(13) Long noncoding RNA GAS5 promotes apoptosis in primary nucleus pulposus cells derived from the human intervertebral disc via Bcl‑2 downregulation and caspase‑3 upregulationMolecular Medicine ReportsJanuary 22, 2019Yifeng Wang, Qingxin Song, Xuan Huang, Zhi Chen, Fan Zhang, Kun Wang, Guofeng Huang, Hongxing ShenScienCell Research Laboratories, Inc. (cat. no. 4800) (20,21), and cultured in NPC medium (cat. no. 4801; both ScienCell Research Laboratories, Inc., San Diego, CA, USA) at 37°C in a humidified atmosphere

(14) Pro-Inflammatory Stimuli Influence Expression of Intercellular Adhesion Molecule 1 in Human Anulus Fibrosus Cells through FAK/ERK/GSK3 and PKCδ Signaling PathwaysInternational Journal of Molecular SciencesDecember 25, 2018Bor-Ren Huang, Da-Tian Bau, Tzu-Sheng Chen, I-Chen Chuang, Cheng-Fang Tsai, Pei-Chun Chang, Horng-Chaung Hsu, Dah-Yuu LuScienCell Research Laboratories (Cat# 4810; Carlsbad, CA, USA). Cells were cultured in NP cell medium with supplements (Cat. #4801; ScienCell Research Laboratories) on poly-L-lysine-coated tissue culture dishes. The medium was changed

(15) EGFR is a pivotal regulator of thrombin-mediated inflammation in primary human nucleus pulposus cultureScientific ReportsDecember 1, 2017Bor-Ren Huang, Tzu-Sheng Chen, Da-Tian Bau, I-Chen Chuang, Cheng-Fang Tsai, Pei-Chun Chang, Dah-Yuu LuCA, USA). Cells were cultured in Nucleus Pulposus Cell Medium with supplements (NPCM, Sciencell, Cat.#4801) on poly-L-lysine coated tissue culture dishes. The media was changed every two days and cells were

(16) Effects of kartogenin on the attenuated nucleus pulposus cell degeneration of intervertebral discs induced by interleukin-1β and tumor necrosis factor-αInternational Journal of Molecular MedicineNovember 24, 2017Yao Huang, Tao Jiang, Jian Chen, Guo-Yong Yin, Jin Fanculture media for the hNPCs is the special media purchased from ScienCell Research Laboratories (cat. no. 4801). The culture media for the mouse intervertebral discs contained 10% fetal bovine serum (Hyclone; GE

(17) Gallic acid inhibits the release of ADAMTS4 in nucleus pulposus cells by inhibiting p65 phosphorylation and acetylation of the NF-κB signaling pathwayOncotargetJuly 18, 2017Yao Huang, Jian Chen, Tao Jiang, Zheng Zhou, Bin Lv, Guoyong Yin, Jin FanTNF-α and GA Human nucleus pulposus cells (ScienCell 4800, USA) and cell-specific media (ScienCell 4801, USA) were purchased from ScienCell. The cells were cultured at 37 °C/5% CO2, and then grown to 90%

(18) Creation of an injectable in situ gelling native extracellular matrix for nucleus pulposus tissue engineeringThe Spine JournalMarch 1, 2017Rebecca A. Wachs, Ella N. Hoogenboezem, Hammad I. Huda, Shangjing Xin, Stacy L. Porvasnik, Christine E. Schmidt1% penicillin-streptomycin, 10% fetal bovine serum, and 1% nucleus pulposus cell growth supplement (#4801, ScienCell Research Laboratories) at 37°C and in normoxic conditions. Nucleus pulposus cells from passage

(19) IL-1β/HMGB1 signalling promotes the inflammatory cytokines release via TLR signalling in human intervertebral disc cellsBioscience ReportsOctober 1, 2016Fang Fang, Dianming JiangScienCell Research Laboratories and were cultured under 5 % CO2 at 37 ◦ C in nucleus pulposus cell medium (NPCM, Cat. No. 4801) (ScienCell Research Laboratories), which were supplemented with 10 % fetal bovine serum (FBS) (Invitrogen),

(20) Link-N Exerts Its Growth Factor-Like Effects through Activation of Smad 1/5 Signaling in Human IVD CellsGlobal Spine JournalMay 1, 2014F. Mwale, P. Madiraju, L. Haglund, P. J. Roughley, J. Antoniouannulus fibrosus (AF) regions. The cells were cultured to 90% confluence in NP Cell Medium (NPCM, Cat. No.4801, ScienCell). Then the cells were incubated overnight in serum-free DMEM followed by treatment with Link-N

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Nucleus Pulposus Cell Medium from ScienCell Research Laboratories

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Nucleus Pulposus Cell Medium from ScienCell Research Laboratories

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