Fig 1: Injection experiment performed in the Teixeira lab.3-6 day old w1118 DrosDel isogenic (Ryder et al., 2004; Chrostek et al., 2013) females were injected with 36.8nL of either buffer (4mM Tris-HCL pH 7.6, 4mM NaCl, 20µM EDTA, 1% (w/v) sucrose and 2% w/v dextran) or a–Actinin in buffer (1µg/µl, Cytoskeleton, #027AT01-A). Flies were collected 24h post infection and RNA extracted from 5 pools of 10 flies per condition, using tripleXtractor reagent (GRiSP, # GB23.0200) followed by DNase treatment (Promega, #M6101). cDNA synthesis was performed with M-MLV Reverse Transcriptase (Promega, #M1705) and Random Primers (Promega, #C1181). cDNA was diluted ten times in DEPC water (Invitrogen, #46-2224) and analysed for gene expression by qPCR using iTaq Universal SYBR Green Supermix (Bio-rad, #1725125). Reactions were carried out using a QuantStudio 7 Flex machine. Relative expression ratios of TotM (A) and TotA (B) were calculated with the Pfaffl method (Pfaffl, 2001), using Rp49 as reference gene and buffer injected flies as control values. TotM and TotA expression is induced by Actinin (linear mixed model, p < 0.001).