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Milk Blockers
Nonfat Dry Milk
Nonfat Dry Milk from Cell Signaling Technology
Be the first to write a review!
Citations:
(103)
Supplier Page
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Cell Signaling Technology for
Nonfat Dry Milk
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Product Specs
Item
Nonfat Dry Milk
Company
Cell Signaling Technology
Price
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Supplier Page
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Catalog Number
9999S
Quantity
250 g
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Cell Signaling Technology
3 Trask Lane
Danvers
,
Massachusetts
01923
United States
Phone
: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488
Company Profile
Website:
http://www.cellsignal.com/
Website:
Technical Support
Citations (103)
(1)
Variants in the CETP gene affect levels of HDL cholesterol by reducing the amount, and not the specific lipid transfer activity, of secreted CETP
PLoS One
December 1, 2023
Åsa Schawlann Ølnes, Marianne Teigen, Jon K. Laerdahl, Trond P. Leren, Thea Bismo Strøm, Katrine Bjune
weight marker. Non-specific binding sites were blocked using 5%
Blotting
Grade Blocker Non-Fat Dry Milk (
9999S
;
Cell Signaling Technology
, Danvers, MA) for 1 h at 22°C. A
horseradish
peroxidase-conjugated mouse
(2)
Exploiting TLK1 and Cisplatin Synergy for Synthetic Lethality in Androgen-Insensitive Prostate Cancer
Biomedicines
November 7, 2023
Siddhant Bhoir, Oluwatobi Ogundepo, Xiuping Yu, Runhua Shi, Arrigo De Benedetti
membrane
was blocked with 5% non-fat dry milk (
Cell Signaling Technology
, Danvers, MA, USA; Cat. No.
9999S
) in 1X Tris-buffered saline with Tween-20 (TBST) for 1 h at room temperature. Following blocking, the
(3)
Critical Velocity, Maximal Lactate Steady State, and Muscle MCT1 and MCT4 after Exhaustive Running in Mice
International Journal of Molecular Sciences
October 30, 2023
Juan B. Orsi, Lara S. Araujo, Pedro P. M. Scariot, Emanuel E. C. Polisel, Luisa O. Cardoso, Claudio A. Gobatto, Fúlvia B. Manchado-Gobatto
of a
Li-COR
Odyssey Fc imaging system. Blocking was performed in 1% milk (skimmed milk powder, cat#
9999
,
Cell Signaling Technology
®, Danvers, MA, USA) in PBS (10 mL of PBS, 0.1 g of milk-NFDM) for 1 h and
(4)
Actin polymerization inhibition by targeting ARPC2 affects intestinal stem cell homeostasis
Burns & Trauma
October 16, 2023
Ruzhen Zhang, Sheng Chen, Zhifan Yang, Ning Zhang, Kenan Guo, Keyi Lv, Zimo Zhou, Meijiao Gao, Xiancheng Hu, Yongping Su,
et al.
Chemical), paraformaldehyde (
Thermo
; 305 362),
EDTA
(Beyotime ST066), nonfat dry milk (
Cell Signaling
9999S
),
goat
serum (Bioss; C-0005),
TRIzol reagent
(
Invitrogen
; 269 201),
bovine serum albumin (BSA
) (Beyotime;
(5)
Synergistic roles of CBX4 chromo and SIM domains in regulating senescence of primary human osteoarthritic chondrocytes
Arthritis Research & Therapy
October 12, 2023
Yu-Hsiu Chen, Xin Zhang, David Attarian, Virginia Byers Kraus
Trans-blot turbo
system (
Bio-Rad
,#
1704274
). The
membrane
was blocked with 5% w/v fat-free milk (
CST
9999
s) for 1 h at room temperature.
Membranes
were washed three times with Tris-buffered saline Tween 20
(6)
Decreased liver B vitamin-related enzymes as a metabolic hallmark of cancer cachexia
Nature Communications
October 6, 2023
Yasushi Kojima, Emi Mishiro-Sato, Teruaki Fujishita, Kiyotoshi Satoh, Rie Kajino-Sakamoto, Isao Oze, Kazuki Nozawa, Yukiya Narita, Takatsugu Ogata, Keitaro Matsuo,
et al.
Tris
-based saline with 0.1%
Tween-20
and 5% w/v nonfat dry milk (
Cell Signaling Technology
MA, USA; Cat#
9999
),
membranes
were incubated overnight with
Malonyl-Lysine [Mal-K] MultiMab Rabbit mAb mix
(
Cell Signaling Technology
(7)
Tripterygium glycosides sensitizes cisplatin chemotherapeutic potency by modulating gut microbiota in epithelial ovarian cancer
Frontiers in Cellular and Infection Microbiology
September 25, 2023
Xinlu Zhan, Qi Zuo, Genhua Huang, Zhanghua Qi, Yufan Wang, Sihong Zhu, Yanying Zhong, Yifei Xiong, Tingtao Chen, Buzhen Tan
transferred onto a 0.22 µm polyvinylidene fluoride
membrane
.
Membranes
were blocked in 5% skimmed milk (
CST
, #
9999
) in Tris-buffered saline with 0.1% Tween 20 (TBST) at room temperature for 90 min. The primary antibody
(8)
Ze-Qi decoction inhibits non-small cell lung cancer growth and metastasis by modulating the PI3K/Akt/p53 signaling pathway
Journal of Traditional and Complementary Medicine
September 1, 2023
Jingtao Zhang, Zifan Zhuang, Minghao Guo, Kai Wu, Qingfeng Yang, Xin Min, Wenqiang Cui, Fei Xu
polyvinylidene difluoride
membranes
(Millipore, MA, USA). The
membranes
were blocked with 5% non-fat dry milk (
9999S
;
Cell Signaling Technology
, Danvers, MA, USA) for 2 h, then incubated with primary antibodies at 4 °C
(9)
(‐)‐Epigallocatechin‐3‐gallate induced apoptosis by dissociation of c‐FLIP/Ku70 complex in gastric cancer cells
Journal of Cellular and Molecular Medicine
September 1, 2023
Mahtab Shahriari Felordi, Mehdi Alikhani, Zahra Farzaneh, Mahmoud Alipour Choshali, Marzieh Ebrahimi, Hamidreza Aboulkheyr Es, Abbas Piryaei, Mustapha Najimi, Massoud Vosough
(
Sigma‐Aldrich
.
P2850
); BCA
assay
(Thermo Fisher Scientific); nonfat dry milk (
Cell Signaling Technology
, #
9999S
) from; ECL
western blotting detection
reagent (Thermo Scientific, RPN2232); 0.1% NP‐40 (Sigma‐Aldrich,
(10)
Single-dose mucosal replicon-particle vaccine protects against lethal Nipah virus infection up to 3 days after vaccination
Sci Adv
August 4, 2023
Stephen R. Welch, Jessica R. Spengler, Sarah C. Genzer, JoAnn D. Coleman-McCray, Jessica R. Harmon, Teresa E. Sorvillo, Florine E. M. Scholte, Sergio E. Rodriguez, T. Justin O'Neal, Jana M. Ritter,
et al.
washer. Wells were blocked for 1 hour at 37°C with blocking buffer [5% milk (
Cell Signaling Technology
,
9999S
) in wash buffer (TBS-T)]. Serially diluted plasma in blocking buffer was added to the blocked plates
(11)
Comprehensive characterization of human brain-derived extracellular vesicles using multiple isolation methods: Implications for diagnostic and therapeutic applications
Journal of extracellular vesicles
August 1, 2023
Zhang Z, Yu K, You Y, Jiang P, Wu Z, DeTure MA, Dickson DW, Ikezu S, Peng J, Ikezu T.
nitrocellulose
membranes
(Cat#
1620115
,
Bio‐Rad
). The
membranes
were blocked with 5% non‐fat milk (Cat#
9999S
,
Cell Signaling Technology
) and incubated with primary antibodies overnight at 4°C. The following primary
(12)
The Extract of Scutellaria baicalensis Attenuates the Pattern Recognition Receptor Pathway Activation Induced by Influenza A Virus in Macrophages
Viruses
July 8, 2023
Yang M, Ma L, Su R, Guo R, Zhou N, Liu M, Wu J, Wang Y, Hao Y.
(No.
ISEQ00010
,
Millipore
, Billrica, MA, USA), then blocked in TBST solution with 5% non-fat milk (No.
9999S
,
CST
, USA) for 1 h. The PVDF
membranes
were incubated with anti-NP (
1:1000
,
mouse
, No.
ab128193
, Abcam,
(13)
Inherited ARPC5 mutations cause an actinopathy impairing cell motility and disrupting cytokine signaling
Nature communications
June 22, 2023
Nunes-Santos CJ, Kuehn H, Boast B, Hwang S, Kuhns DB, Stoddard J, Niemela JE, Fink DL, Pittaluga S, Abu-Asab M,
et al
.
(Invitrogen). All
membranes
were blocked in 5% (wt/vol) of nonfat dry milk (
Cell Signaling Technology
, Cat.
9999
) in 1X Tris-buffered saline with 0.1% Tween 20 (TBS-
T, Cell
Signaling Technology, Cat. 9997), incubated
(14)
Evaluation of therapeutic PD-1 antibodies by an advanced single-molecule imaging system detecting human PD-1 microclusters
Nature communications
June 6, 2023
Nishi W, Wakamatsu E, Machiyama H, Matsushima R, Saito K, Yoshida Y, Nishikawa T, Takehara T, Toyota H, Furuhata M,
et al
.
citrate buffer, pH 4.5, for 24 h at 37 °C, blocked with 5% nonfat dried milk (
Cell Signaling Technology
,
9999S
) in PBS for 30 min at 37 °C, removed the cover glass, and left to stand in the
assay
medium (Hepes-buffered
(15)
SPINK2 Protein Expression Is an Independent Adverse Prognostic Marker in AML and Is Potentially Implicated in the Regulation of Ferroptosis and Immune Response
International Journal of Molecular Sciences
June 2, 2023
Herbert Augustus Pitts, Chi-Keung Cheng, Joyce Sin Cheung, Murphy Ka-Hei Sun, Yuk-Lin Yung, Hoi-Yun Chan, Raymond S. M. Wong, Sze-Fai Yip, Ka-Ngai Lau, Wai Shan Wong,
et al.
for 1 h at room temperature with 5% non-fat dry milk (
Cell Signaling Technology
, Danvers, MA, USA, #
9999
) in TBS TweenTM 20 Buffer (#
28360
,
ThermoFisher
). This was followed by
incubation
with primary antibodies
(16)
Activating an Adaptive Immune Response with a Telomerase-Mediated Telomere Targeting Therapeutic in Hepatocellular Carcinoma
Molecular cancer therapeutics
June 1, 2023
Mender I, Siteni S, Barron S, Flusche AM, Kubota N, Yu C, Cornelius C, Tedone E, Maziveyi M, Grichuk A,
et al
.
Membranes
were blocked with TBST buffer containing 5% skim milk (
Cell Signaling Technology
, Catalog No.
9999
) for 1 hour and incubated overnight at 4°C with various primary antibodies (
1:1,000
). Following three
(17)
FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicity
Cell metabolism
May 2, 2023
Wieder N, Fried JC, Kim C, Sidhom EH, Brown MR, Marshall JL, Arevalo C, Dvela-Levitt M, Kost-Alimova M, Sieber J,
et al
.
(#
1704155
,
BioRad
) according to the manufacturer’s protocol.
Membranes
were blocked in 5%
Nonfat Dry Milk
(#
9999S
,
Cell Signaling Technology
) in PBS with 0.1% Tween 20 (PBS-T).
AKT
and pAKT blots were blocked instead
(18)
Super Carbonate Apatite-miR-497a-5p Complex Is a Promising Therapeutic Option against Inflammatory Bowel Disease
Pharmaceuticals (Basel)
April 19, 2023
Naoto Tsujimura, Takayuki Ogino, Masayuki Hiraki, Taisei Kai, Hiroyuki Yamamoto, Haruka Hirose, Yuhki Yokoyama, Yuki Sekido, Tsuyoshi Hata, Norikatsu Miyoshi,
et al.
#
1620177
). The
membranes
were blocked with 5% non-fat dry milk (
Cell Signaling Technology
, Inc. Cat, No. #
9999
, Beverly, MA, USA) in TBS with Tween-20 (TBS-T; 50 mM Tris, 158 mM NaCl, 2.7 mM KCl, pH 7.5, 0.1% Tween-20)
(19)
Aberrant somatic calcium channel function in cNurr1 and LRRK2-G2019S mice
npj Parkinson's Disease
April 7, 2023
Olga Skiteva, Ning Yao, Ioannis Mantas, Xiaoqun Zhang, Thomas Perlmann, Per Svenningsson, Karima Chergui
for 1 h at room temperature with 5% (w/v) fat-free dry milk (
Cell Signaling
, Danvers, USA, Cat. No.
9999
S) in TBS-T (Tris base 0.05 mol/L, NaCl 0.15 mol/L, tween 0.1%).
Immunoblotting
was carried out with
(20)
FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicity
bioRxiv : the preprint server for biology
February 20, 2023
Wieder N, Fried JC, Kim C, Sidhom EH, Brown MR, Marshall JL, Arevalo C, Dvela-Levitt M, Kost-Alimova M, Sieber J,
et al
.
(#
1704155
,
BioRad
) according to the manufacturer’s protocol.
Membranes
were blocked in 5%
Nonfat Dry Milk
(#
9999S
,
Cell Signaling Technology
) in PBS with 0.1% Tween® 20 (PBS-T).
AKT
and pAKT blots were blocked instead
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Nonfat Dry Milk from Cell Signaling Technology
Supplier Page
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Pricing Info
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