KingFisher™ Duo Prime Purification System from Thermo Fisher Scientific

Product Specs
ItemKingFisher™ Duo Prime Purification System
CompanyThermo Fisher Scientific
Price Get Pricing Get Quote for this Product
Catalog Number5400110
Capacityup to 12 (12-pin magnet head); up to 6 (6-pin magnet head)
QuantityEA
ApplicationsDNA/RNA Purification
Dimensions(WxDxH) 40 x 46 x 35 cm (15.8 x 16.1 x 17.7 inches)
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures.
Volume96 Deep well-plate : 50 to 1000µL
24 Deep well-plate : 200 to 5000µL
Elution strip : 30 to 130µL

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Detection of Novel US Neisseria meningitidis Urethritis Clade Subtypes in JapanEmerging Infectious DiseasesNovember 1, 2023Hideyuki Takahashi, Masatomo Morita, Mitsuru Yasuda, Yuki Ohama, Yoshitomo Kobori, Munekado Kojima, Ken Shimuta, Yukihiro Akeda, Makoto Ohnishigenomic DNA by using the MagMAX DNA Multi-Sample Ultra 2.0 Kit, which we then purified by using the KingFisher Duo Prime Purification System and measured concentrations by using a Qubit dsDNA HS assay kit (all from Thermo Fisher Scientific,

(2) Peripheral blood mononuclear cells exhibit increased mitochondrial respiration after adjuvant chemo‐ and radiotherapy for early breast cancerCancer MedicineAugust 1, 2023Ida Bager Christensen, Marie‐Louise Abrahamsen, Lucas Ribas, Kristian Buch‐Larsen, Djordje Marina, Michael Andersson, Steen Larsen, Peter Schwarz, Flemming Dela, Linn Gillbergrespirometry was performed using the Mag‐Bind® Blood and Tissue DNA HDQ 96 Kit (Omega Bio‐Tek) and the KingFisher™ Duo Prime purification system (Thermo Scientific™). DNA concentration was measured using Qubit Fluorometric Quantification with the

(3) SAPrIm, a semi-automated protocol for mid-throughput immunopeptidomicsFrontiers in ImmunologyJune 2, 2023Terry C. C. Lim Kam Sian, Gabriel Goncalves, Joel R. Steele, Tima Shamekhi, Liesl Bramberger, Dongbin Jin, Mohammad Shahbazy, Anthony W. Purcell, Sri Ramarathinam, Stoyan Stoychev, et al.#10812846001) 2.2 Additional materials/equipment Tissuelyser LT (Qiagen #85600)Kingfisher Duo (Thermo Scientific #5400110)C18 stage tips (Thermo Scientific #87784)KingFisher tip comb (Thermo Scientific #97003500)KingFisher

(4) SARS-CoV-2 viral variants can rapidly be identified for clinical decision making and population surveillance using a high-throughput digital droplet PCR assayScientific ReportsMay 10, 2023Olivier Pernet, Maia Weisenhaus, Chrysovalantis Stafylis, Christopher Williams, Mihaela Campan, Jonas Pettersson, Nicole Green, David M. Lee, Paul D. Thomas, Pamela Ward, et al.Extraction was done on either the KingFisher Apex Purification System (Cat#5,400,930, ThermoScientific) or KingFisher Duo Prime Purification System (Cat#5,400,110, ThermoScientific). Samples were eluted into 75μL of nuclease-free water and stored at

(5) Streamlined high-throughput cloning protocol to generate arrayed mutant librariesSTAR ProtocolsMarch 1, 2023Kerry T. Sun, Tark S. Patel, Justin Kim, Helen S.H. Tang, Ghazaleh Eskandari-Sedighi, Haresh Sureshkumar, Dean Schieve, S.A. Mokfreezing competent cells),Thomas Scientific,Cat#1165R30 Kingfisher Duo Prime,Thermo Fisher Scientific,Cat#5400110 Benchtop Refrigerated Centrifuge 5810R with S-4-104 rotor,Eppendorf,Cat#022628092 "Plate bucket adaptors

(6) LINKIN-associated proteins necessary for tissue integrity during collective cell migrationbioRxiv : the preprint server for biologyFebruary 9, 2023Tan CH, Cheng KW, Park H, Sternberg PW, Chou TF.extracted from cells using the MagMAX mirVana Total RNA Isolation Kit (Thermo Scientific, A27828) with KingFisher Duo Prime Purification System (Thermo Scientific). Total RNA was converted to cDNA using High-Capacity cDNA Reverse Transcription

(7) Accurate Detection of SARS-CoV-2 by Next-Generation Sequencing in Low Viral Load SpecimensInternational Journal of Molecular SciencesFebruary 9, 2023Marius Ilié, Jonathan Benzaquen, Véronique Hofman, Elodie Long-Mira, Sandra Lassalle, Jacques Boutros, Christophe Bontoux, Virginie Lespinet-Fabre, Olivier Bordone, Virginie Tanga, et al.MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit ((#A42352, Applied Biosystems, Foster City, CA, USA) using the KingFisher™ Duo Prime Purification System (Thermo Fisher Scientific, Waltham, MA, USA). In order to prepare the normalization of RNA samples before

(8) Assessing nuclear versus mitochondrial cell-free DNA (cfDNA) by qRT-PCR and droplet digital PCR using a piglet model of perinatal asphyxiaMolecular Biology ReportsFebruary 1, 2023Marie Bitenc, Benedicte Grebstad Tune, Maria Melheim, Monica Atneosen-Åsegg, Xiaoran Lai, Polona Rajar, Rønnaug Solberg, Lars Oliver Baumbuschextracted from plasma using the Mag-Bind cfDNA Kit (Omega Biotek), following the recommendations of the KingFisher Duo Prime Purification System (Thermo Fisher Scientific). Due to the low input starting volume of 450 μl, some modifications to the

(9) Different impact of bovine complement regulatory protein 46 (CD46_bov) as a cellular receptor for members of the species Pestivirus H and Pestivirus GEmerging Microbes & InfectionsDecember 31, 2022Elena Leveringhaus, Gökce Nur Cagatay, Juliane Hardt, Paul Becher, Alexander PostelMDBKΔCD46 cell line, cellular RNA was extracted from MDBK wild type (WT) and MDBKΔCD46 cells using the KingFisher™ Duo Prime Purification System (Thermo Fisher Scientific) and the IndiMag® Pathogen Kit (SP947457, Indical Bioscience) according to

(10) Pathogen Exposure in White Whales (Delphinapterus leucas) in Svalbard, NorwayPathogensDecember 29, 2022Ingebjørg H. Nymo, Ursula Siebert, Christine Baechlein, Alexander Postel, Eva M. Breines, Christian Lydersen, Kit M. Kovacs, Morten Trylandwas 5158. 2.2. Extraction of RNA Nucleic acid was extracted from serum samples (n = 27) using the KingFisher Duo Prime Purification System (ThermoFisher Scientific, Waltham, MA, USA) and the IndiMag Pathogen Kit (Indical Bioscience, Leipzig,

(11) Labeled TEMPO-Oxidized Mannan Differentiates Binding Profiles within the Collectin FamiliesInternational Journal of Molecular SciencesDecember 16, 2022Florent Le Guern, Anne Gaucher, Gina Cosentino, Marion Lagune, Henk P. Haagsman, Anne-Laure Roux, Damien Prim, Martin RottmanAvidities and competitions assessments were performed using magnetic beads operated by a Thermo Scientific KingFisher Duo Prime purification system (ThermoFisher, Illkirch, France). For ELISA-like avidity array assays, the absorbance of oxidated TMB

(12) PAC-FragmentDEL – photoactivated covalent capture of DNA-encoded fragments for hit discovery†RSC Medicinal ChemistryNovember 16, 2022Huiyong Ma, James B. Murray, Huadong Luo, Xuemin Cheng, Qiuxia Chen, Chao Song, Cong Duan, Ping Tan, Lifang Zhang, Jian Liu, et al.commercially available See ESI.† Screening, sequencing, and decoding Selection was carried out using a KingFisher Duo Prime Purification System (ThermoFisher) in a 96 well plate. 2.5 μM PAK4 (or 2-epimerase) was incubated with a mixture of the

(13) Is the Illegal Trade of Glass Eels (Anguilla anguilla) Increasing the Spread of Disease? A Case of EVEXMicroorganismsNovember 8, 2022Ivana Giovanna Zupičić, Dražen Oraić, Željko Pavlinec, Dinko Novosel, Luka Žuvić, Tanja Šegvić-Bubić, Snježana Zrnčićmolecular analysis (Applied Biosystems, Waltham, MA, USA) according to the manufacturer’s protocol on a KingFisher Duo Prime Purification System (Thermo Scientific, Waltham, MA, USA). 2.4.3. PCR Testing for the Presence of AnHV Extracted DNA was

(14) Dearth of influenza among older adults admitted with respiratory symptoms in Malaysia during the coronavirus disease 2019 pandemic in 2021Frontiers in MedicineOctober 10, 2022Maw Pin Tan, Chee Loon Leong, Yong Kek Pang, Rizah Mazzuin Razali, Ahmad Izuanuddin Ismail, I-Ching Sam, Rosilawati Abdul Rani, Jennifer Chong, Mohd Arif Mohd Zim, Aisya Natasya Musa, et al.Medical Device Authority, Malaysia. For extraction, KLGH, HT and SH used RNA Isolation Kit with the KingFisher Duo Prime Purification System (Thermo Fisher Scientific, USA), Viral RNA Isolation Kit with the Liferiver EX3600 (Liferiver Biotech,

(15) Quantitative reverse transcription PCR assay to detect a genetic marker of pyrethroid resistance in Culex mosquitoesPLOS ONEAugust 8, 2022Kelli M. Hager, Erick Gaona, Amy Kistler, Kalani Ratnasiri, Hanna Retallack, Miguel Barretto, Sarah S. Wheeler, Christopher M. Hoover, Eric J. Haas-Stapletonwas extracted using the MagMAX-96 Viral RNA Isolation Kit (which isolates both RNA and DNA) and the KingFisher Duo Prime Purification System programed with the MagMAX Pathogen Standard Volume software protocol as described by the manufacturer

(16) A Genome for Edith’s Checkerspot Butterfly: An Insect with Complex Host-Adaptive Suites and Rapid Evolutionary Responses to Environmental ChangesGenome Biology and EvolutionAugust 3, 2022Kalle Tunstrom, Christopher W Wheat, Camille Parmesan, Michael C Singer, Alexander S Mikheyevextracted using KingFisher Cell and Tissue DNA Kit from ThermoFisher scientific (N11997) using the robotic Kingfisher Duo Prime purification system. DNA quality was assessed using 260/280 ratio (Nanodrop 8000 spectrophotometer; Thermo Scientific, MA,

(17) Multifaceted Assessment of Wastewater-Based Epidemiology for SARS-CoV-2 in Selected Urban Communities in Davao City, Philippines: A Pilot StudyInternational Journal of Environmental Research and Public HealthJuly 19, 2022Maria Catherine B. Otero, Lyre Anni E. Murao, Mary Antoinette G. Limen, Daniel Rev A. Caalim, Paul Lorenzo A. Gaite, Michael G. Bacus, Joan T. Acaso, Refeim M. Miguel, Kahlil Corazo, Ineke E. Knot, et al.MagMax Viral/Pathogen Nucleic Acid Isolation Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA) in a Kingfisher Duo Prime Purification System (Thermo Fisher Scientific Inc., USA). RT-PCR detection of SARS-CoV-2 was performed in duplicate using

(18) Expanding the DNA-encoded library toolbox: identifying small molecules targeting RNANucleic Acids ResearchJuly 8, 2022Qiuxia Chen et al.HitGen. DEL selection HitGen DELs were used in the selection and automated selection was carried out using a KingFisher Duo Prime Purification System (ThermoFisher) in 96-well plate. 10 μM biotin-TAR RNA was incubated with 10.38 billion member DEL sample

(19) Genome Sequences of Five Arenaviruses from Pygmy Mice (Mus minutoides) in Sierra LeoneMicrobiology Resource AnnouncementsMay 19, 2022Matej Vučak, James Bangura, Bruno M. Ghersi, Jenna Nichols, Joseph Hughes, Ana da Silva Filipe, Alexandre Tremeau-Bravard, David J. Wolking, Emmanuel Amara, Abdulai Bangura, et al.each mouse. Nucleic acids were extracted using Applied Biosystems MagMAX CORE and pathogen kits with a KingFisher Duo Prime purification system and treated with Ambion DNase I. RNA was purified using the Beckman Coulter RNAClean XP kit and depleted

(20) SARS-CoV-2 circulation in Croatian wastewaters and the absence of SARS-CoV-2 in bivalve molluscan shellfishEnvironmental ResearchMay 1, 2022Dragan BrniÄ‡, Ivana LojkiÄ‡, Ines Å koko, Nina KreÅ¡iÄ‡, Ivana Å imiÄ‡, Tomislav Keros, Marin Ganjto, Dario Å tefanac, Branka Viduka, Dario KarÅ¡aj, et al.following manufacturer's instructions. The extraction procedure for MagMAX™ CORE kit was automated using KingFisher™ Duo Prime purification system (ThermoFisher Scientific, USA). The extracted nucleic acids were stored at −80 °C or processed immediately

Write a Review

(1)

4/10/2020Great Purification System for Nucleotide Acids by Thermo Fisher Patricia FrancoI really consider that this device meets the expectations for what was created in a very efficient way, so I really recommend it in any laboratory that merits its use.
(1)

11/4/2016KingFisher System - Perfect Solution For Customizable Automated Experiment Design Simen ZhaoThe aim of my research is the study of RNA modifications, which requires a lot of parallel RNA purification and IP experiments. KingFisher enables me to process up to 12 samples at a time with ... Read Review

Is It Time to Rethink Your Nucleic Acid Purification?
January 16, 2015
Purifying high quality DNA and RNA is critical for ensuring that the downstream applications go as smoothly as possible. Unfortunately the purification process itself can be time consuming and tedious which can ... read more