Description
Dynabeads M-280 Streptavidin beads are 2.8-µm monosized, monodispersed, superparamagnetic, and hydrophobic beads covalently coupled to streptavidin.
Dynabeads M-280 Streptavidin beads are 2.8-µm monosized, monodispersed, superparamagnetic, and hydrophobic beads covalently coupled to streptavidin. They are blocked with BSA and intended for use in the isolation and handling of biotinylated nucleic acids, antibodies, and other biotinylated ligands. The excellent dispersion abilities and the absence of magnetic remanence make the Dynabeads streptavidin products ideal for both manual and automated protocols, including microfluidic system protocols.
Benefits of Dynabeads M-280 Streptavidin beads include:
• Performance—high sensitivity and reproducibility secure consistent results
• Efficiency—fast binding kinetics and high binding capacity
• Flexibility—flexible protocols with gentle and efficient liquid-phase reaction kinetics
• Automation ready—easy automation for high-throughput needs on the Kingfisher Purification System
Consistency and reproducibility
The monolayer of streptavidin leaves most biotin-binding sites available for the binding of free biotin and biotinylated ligands. The specific and defined surface allows for efficient capture, separation, and downstream handling. In addition, the low size distribution (<5%) of the monosized beads and the streptavidin monolayer ensure negligible leakage, providing batch consistency and reproducibility of results.
High binding capacity
The size of the ligand and the biotinylation procedure will affect the binding capacity. The capacity also depends on steric availability and charge interaction between bead and ligand and between ligands. With two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization, one mg of Dynabeads M-280 Streptavidin typically binds:
• 490-750 pmol free biotin
• 10 µg biotinylated IgG
• ∼200 pmol biotinylated peptides
• ∼10 µg biotinylated ds-DNA
• ∼200 pmol biotinylated ss-oligonucleotides
Applications
Thermo Fisher streptavidin-coupled Dynabeads magnetic beads have been used and cited for a wide variety of applications. Although the beads can be used to isolate any biotinylated targets, these hydrophobic beads are particularly well suited for immunoassays, protein purification, cell isolation, phage display, and biopanning.
Automation ready with the KingFisher Purification System
The 2.8-µm magnetic beads are ideal for high-throughput enrichment. This process can be automated using any of our KingFisher Sample Purification Systems or liquid handlers.
Commercial supply
Our manufacturing sites are ISO 13485-certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are in the process of customizing Dynabeads M-280 Streptavidin beads in a commercial product or service or need a larger bulk volume of this product, please contact us or see our Dynabeads OEM page.