HpyCH4V from New England Biolabs

Product Specs
ItemHpyCH4V
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0620L
Quantity500 units
Concentration5,000 units/ml
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

TG/CA

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Single duplex DNA sequencing with CODEC detects mutations with high sensitivityNature GeneticsMay 1, 2023Jin H. Bae, Ruolin Liu, Eugenia Roberts, Erica Nguyen, Shervin Tabrizi, Justin Rhoades, Timothy Blewett, Kan Xiong, Gregory Gydush, Douglas Shea, et al.65 °C for 30 min. We modified ddBTP-blocked ER/AT22 to start with fragmentation with HpyCH4V (NEB, R0620S) and AluI (NEB, R0137S; Extended Data Fig. 6b) in NEB CutSmart buffer at 37 °C for 15 min, purified

(2) ChIATAC is an efficient strategy for multi-omics mapping of 3D epigenomes from low-cell inputsNature CommunicationsJanuary 13, 2023Haoxi Chai, Harianto Tjong, Peng Li, Wei Liao, Ping Wang, Chee Hong Wong, Chew Yee Ngan, Warren J. Leonard, Chia-Lin Wei, Yijun Ruanincubated at 37 °C for 20 min. Cells were then in situ digested by AluI (NEB; R0137L) or AluI+HpyCH4V (NEB; R0620L) restriction enzymes at 37 °C for at least 2 h or overnight by adding 2 μl AluI/ 1 μl AluI + 1 μl HpyCH4V,

(3) Reversible epigenetic alterations regulate class I HLA loss in prostate cancerCommunications BiologySeptember 1, 2022Tamara S. Rodems et al.10 units/µL (New England Biolabs, Cat# R0137L) and HpyCH4V at 5 units/µL (New England Biolabs, Cat# R0620L)) in 20 µL reactions containing 1x CutSmart Buffer (New England Biolabs, Cat# B7204S) for 15 min at

(4) Somatic mutation landscapes at single-molecule resolutionNatureMay 20, 2021Federico Abascal et al.in: https://github.com/cancerit/NanoSeq https://github.com/fa8sanger/NanoSeq_Paper_Code Introduction Reagents NEBNext® Ultra™ II Q5® Master Mix (NEB: M0544L) HpyCH4V (NEB: R0620L) T4 DNA Ligase (NEB: M0202L) dATP Solution (NEB: N0440S) xGen CS Adapter - Tech Access (IDT: 1080799) CutSmart® Buffer

(5) Mapping the epigenomic and transcriptomic interplay during memory formation and recall in the hippocampal engram ensembleNature NeuroscienceDecember 1, 2020Asaf Marco et al.Life Technologies, 11205D). For the second restriction enzyme we used the 4-base cutter Hpych4V (10 U /per sample, R0620S, New England Biolabs, USA). After A-tailing reaction (see original protocol for details), samples were

(6) Anosmin1 Shuttles Fgf to Facilitate Its Diffusion, Increase Its Local Concentration, and Induce Sensory OrgansDevelopmental CellSeptember 1, 2018John Wang, Yandong Yin, Stephanie Lau, Jagadish Sankaran, Eli Rothenberg, Thorsten Wohland, Martin Meier-Schellersheim, Holger Knaut"Alexa Fluor 594 Carboxylic Acid, tris(triethylammonium) salt",Thermo Fisher,A33082 BsrI,New England Biolabs,R0527S Hpych4v,New England Biolabs,R0620L AciI,New England Biolabs,R0551S ApoI,New England Biolabs,R0566L Taqαi,New England Biolabs,R0149L Brefeldin

(7) Genome-wide mapping of autonomous promoter activity in human cellsNature BiotechnologyFebruary 1, 2017Joris van Arensbergen, Vincent D. FitzPatrick, Marcel de Haas, Ludo Pagie, Jasper Sluimer, Harmen J. Bussemaker, Bas van Steenseldigested for 1 hour with 10 units of frequent cutter Nla III (#R0125S; NEB) or 10 units of HpyCH4V (#R0620L; NEB), bead purified and self-ligated again in a final volume of 1 ml. This material was purified by

(8) In silico analysis identifies novel restriction enzyme combinations that expand reduced representation bisulfite sequencing CpG coverageBMC Research NotesDecember 1, 2014Daniel B Martinez-Arguelles, Sunghoon Lee, Vassilios Papadopoulosnumber AluI,AG|CT,37,CutSmart,80,No,R0137S BfaI,C|TAG,37,CutSmart,80,No,R0568S HaeIII,GG|CC,37,CutSmart,80,No,R0108S HpyCH4V,TG|CA,37,CutSmart,65,No,R0620S MluCI,|AATT,37,CutSmart,No,No,R0538S MseI,T|TAA,37,CutSmart,65,No,R0525S MspI,C|CGG,37,CutSmart,No,No,R0106S TaqI,T|CGA,65,CutSmart,80,dam,R0149S CviQI,G|TAC,25,NEB

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HpyCH4V

HpyCH4V from New England Biolabs

Description

Sequence

New England Biolabs

Citations (8)

Reviews

HpyCH4V from New England Biolabs

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