HpaII Methyltransferase from New England Biolabs

Product Specs
ItemHpaII Methyltransferase
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0214S
Concentration4,000 units/ml
Quantity100 units
ApplicationsFunctional Genomics
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeEnzymes
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to protect 1 µg lambda DNA in 1 hour at 37°C in a total reaction volume of 10 µl against cleavage by HpaII restriction endonuclease.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Accurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPSGenome BiologyDecember 1, 2020Yibin Liu et al.PCR amplification of pNIC28-Bsa4 plasmid (Addgene, cat. no. 26103) and methylated by HpaII Methyltransferase (NEB, M0214S). Unmethylated lambda DNA (Promega) was also methylated by HpaII Methyltransferase for CmCGG methylation. Detailed

(2) Antagonistic control of DDK binding to licensed replication origins by Mcm2 and Rad53eLifeJuly 23, 2020Syafiq Abd Wahab, Dirk Remusprotein",Rad53D339A(6xHis-Rad53D339A),PMID:32341532,, "Peptide, recombinant protein",HpaII methyltransferase,NEB,Cat. #: M0214S, Commercial assay or kit,SilverQuest Silver Staining Kit,Invitrogen (ThermoFisher),Cat. #: LC6070, "Chemical

(3) Copper bis-Dipyridoquinoxaline Is a Potent DNA Intercalator that Induces Superoxide-Mediated Cleavage via the Minor GrooveMoleculesNovember 26, 2019Zara Molphy, Vickie McKee, Andrew Kellettenzymes R0171S), MspI (NEB, R0106S) and HpaII-MT of400 400ng ngofofthe theamplified amplified sequence (NEB, R0106S) and HpaII-MT(NEB, (NEB,M0214S). M0214S). A A quantity quantity of sequence waswas treated with eachenzyme enzymefor for1 1h hatat3737◦

(4) Molecular Basis for ATP-Hydrolysis-Driven DNA Translocation by the CMG Helicase of the Eukaryotic ReplisomeCell ReportsSeptember 3, 2019Patrik Eickhoff et al.2017",N/A Ctf4,"Gambus et al., 2009",N/A Pol epsilon(exo-),"Goswami et al., 2018",N/A M.HpaII,NEB,M0214S ,, Deposited Data,Deposited Data,Deposited Data ,, Drosophila CMG-DNA state 1A cryo-EM map,This

(5) DNA base flipping analytical pipelineBiology Methods and ProtocolsAugust 11, 2017Peng Zhang, Florian D. Hastert, Anne K. Ludwig, Kai Breitwieser, Maria Hofstätter, M. Cristina Cardoso(Clontech Laboratories, USA) were used. M.HpaII methyltransferase was purchased from NEB (cat. no. M0214S). Electrophoretic mobility shift analysis Purified proteins were incubated with the different fluorescently

(6) Hormone stimulation of androgen receptor mediates dynamic changes in DNA methylation patterns at regulatory elementsOncotargetDecember 15, 2015Vineet K. Dhiman, Kristopher Attwood, Moray J. Campbell, Dominic J. Smiraglia(New England Biolabs, Cat# M0226S) for total methylation and HpaII methyltransferase (New England Biolabs, Cat# M0214S) for partial methylation. Samples were then mixed and incubated overnight at 37°C. Afterwards, each

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