Deep VentDNA Polymerase from New England Biolabs

Product Specs
ItemDeep VentDNA Polymerase
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0258S
Formatunits/ml
Concentration2,000 units/ml
Quantity200 units
ApplicationsDNA Analysis
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeDNA Polymerase
Temperature-20 C

Specifications/Features

Comments:
Item name is changed

Buffer:
1X ThermoPol Reaction Buffer:

20 mM Tris-HCl

10 mM (NH₄)₂SO₄

10 mM KCl

2 mM MgSO₄

0.1 % Triton X-100

pH 8.8 @ 25°C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Fluorescent Protein Variants Generated by Reassembly between Skeleton and ChromophoreACS OmegaFebruary 2, 2021Tingting Sun, Tianpeng Li, Ke Yi, Guoquan Yan, Xiaolian Gao(Sangon Biotech, Shanghai, China, Table S2). PCR reactions were performed using DeepVent polymerase (M0258L, NEB) as described in the Supporting Information Methods. The amplified products were digested with

(2) A cell-free antibody engineering platform rapidly generates SARS-CoV-2 neutralizing antibodiesbioRxiv : the preprint server for biologyOctober 30, 2020Chen X, Gentili M, Hacohen N, Regev A.Phusion DNA polymerase (New England Biolabs, M0530L) and Deep Vent DNA polymerase (New England Biolabs, M0258L) were used for both PCRs to ensure efficient amplification. PCR cycle number was chosen to avoid over-amplification

(3) Chronic exposure of a freshwater mussel to elevated p CO ₂ : Effects on the control of biomineralization and ion‐regulatory responsesEnvironmental Toxicology and ChemistryFebruary 1, 2018Jennifer D. Jeffrey, Kelly D. Hannan, Caleb T. Hasler, Cory D. SuskiµL) were 2 µL cDNA, 0.2 µM primer, and 5 µL Taq th o 5X Master Mix (Taq 5X Master Mix, catalog no. M0258L; New England BioLabs). Cycling conditions were 95 °C (30 s), 55 °C (30 s), and 68 °C (30 s) for 38 cycles

(4) An Inducible Alpha-Synuclein Expressing Neuronal Cell Line Model for Parkinson's Disease1Journal of Alzheimer's disease : JADJanuary 1, 2018Vasquez V, Mitra J, Perry G, Rao KS, Hegde ML.bluntended full-length α-Syn amplified from pcDNA-WT-α Syn plasmid using Deep Vent DNA polymerase (M0258, NEB) into a pCW-Cas9 vector (gift from Eric Lander and David Sabatini, Addgene plasmid 50661) [19]

(5) Examining Sources of Error in PCR by Single-Molecule SequencingPLOS ONEJanuary 6, 2017Vladimir Potapov, Jennifer L. OngDNA Polymerase (#M0491), Phusion High Fidelity DNA Polymerase (#M0530), Deep Vent DNA Polymerase (#M0258), and Taq DNA polymerase (#M0273) are supplied by New England Biolabs. Other polymerases were purchased

(6) Chromatin-Bound Oxidized α-Synuclein Causes Strand Breaks in Neuronal Genomes in in vitro Models of Parkinson's DiseaseJournal of Alzheimer's disease : JADJanuary 1, 2017Vasquez V, Mitra J, Hegde PM, Pandey A, Sengupta S, Mitra S, Rao KS, Hegde ML.blunt-ended full-length α-Syn amplified from pcDNA-WT-α-Syn plasmid using Deep Vent DNA polymerase (M0258, NEB) into pCWCas9 vector (gift from Eric Lander and David Sabatini, Addgene plasmid 50661) [32] and digested

(7) BB0347, from the Lyme Disease Spirochete Borrelia burgdorferi, Is Surface Exposed and Interacts with the CS1 Heparin-Binding Domain of Human FibronectinPLoS ONESeptember 27, 2013Robert A. Gaultney, Tammy Gonzalez, Angela M. Floden, Catherine A. Brissetterespectively. Recombinant Protein Production DNA sequences were amplified via PCR using DeepVent DNA Polymerase (NEB, #M0258S; Ipswich, MA) to prevent addition of the 3′ A-overhang onto amplified nucleotide segments. Primers used

(8) Identification of Signaling Protein Complexes by Parallel Affinity Precipitation Coupled with Mass SpectrometryCell Communication InsightsSeptember 11, 2013Heng Lu, Qishan Lin, Jihe Zhao(for HA-tag);1,7–10pHAN mammalian expression vector (for Myc-tag);1,11,12Deep Vent DNA polymerase (#M0258S; New England Biolabs, Ipswich, MA, USA);T4 DNA ligase (#M0202S; New England Biolabs);Aurum Plasmid Mini Kit

(9) Effective modifications for improved homologous recombination and high-efficiency generation of recombinant adenovirus-based vectorsJournal of Virological MethodsNovember 1, 2008Chengxiang Wu, Vivek R. Nerurkar, Richard Yanagihara, Yuanan Lu5′-gcatcaagaacagaagtatgtc-3′ . In this PCR amplification, Deep Vent DNA polymerase (NEB, Beverly, MA, Cat. No. M0258S) was used and the PCR product was directly ligated into pShuttle-CMV-GFP DNA that had been linearized

Be the First to Write a Review!