MagneGST Protein Purification System from Promega

Product Specs
ItemMagneGST Protein Purification System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV8603
Quantity200 reactions
MethodMagnetic
Product TypeResins, Magnetic Beads
ModeAffinity Purification
Applicationspurification

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) The GhMAP3K62-GhMKK16-GhMPK32 kinase cascade regulates drought tolerance by activating GhEDT1-mediated ABA accumulation in cottonJournal of Advanced ResearchSeptember 1, 2023Lin Chen, Bing Zhang, Linjie Xia, Dandan Yue, Bei Han, Weinan Sun, Fengjiao Wang, Keith Lindsey, Xianlong Zhang, Xiyan YangHis-GhMPK32. The pull-down proteins were purified with MagneGSTTM Protein Purification System (Promega V8603) and MagneHisTM Protein Purification System (Promega V8550). The pull-down assay was performed as described

(2) A cap 0-dependent mRNA capture method to analyze the yeast transcriptomeNucleic Acids ResearchDecember 9, 2022Martyna Nowacka, Przemysław Latoch, Matylda A Izert, Natalia K Karolak, Rafal Tomecki, Michał Koper, Agnieszka Tudek, Agata L Starosta, Maria W Górnacapture using human eIF4E(K119A) or yeast Cdc33 or Cbp80-20 50 μl of MagneGST magnetic beads (Promega V8600) per 1 sample were washed 3 times with 1 ml of PBS. The GST binding capacity declared by the manufacturer

(3) Co-crystalization reveals the interaction between AtYchF1 and ppGppFrontiers in Molecular BiosciencesNovember 30, 2022Ming-Yan Cheung, Xiaorong Li, Yee-Shan Ku, Zhongzhou Chen, Hon-Ming LamExcellose® spin kit (Cat# 23020, Mbiotech, Haman, Korea) and MagneGST™ Protein Purification System (Cat# V8600, Promega, CA, United States) were then used to purify the expressed MBP- and GST-tagged proteins according

(4) African Swine Fever Virus EP364R and C129R Target Cyclic GMP-AMP To Inhibit the cGAS-STING Signaling PathwayJournal of VirologyAugust 10, 2022Niranjan Dodantenna et al.purification. Glutathione protein was purified using the MagneGST protein purification system (Promega; V8600) with minor modifications. Briefly, GST-tagged C129R, EP364R-WT, and EP364R-MT pEGB plasmids were transfected

(5) Cathepsin D interacts with adenosine A_2A receptors in mouse macrophages to modulate cell surface localization and inflammatory signalingThe Journal of biological chemistryMay 1, 2022Skopál A, Kéki T, Tóth PÁ, Csóka B, Koscsó B, Németh ZH, Antonioli L, Ivessa A, Ciruela F, Virág L, et al.no.: 114-15; Polyplus transfection); GST-specific MagneGST Protein Purification System (catalog no.: V8600; Promega); 8-well tissue culture–treated glass chamber slides (catalog no.: 354118; Falcon); 96-well

(6) The Zinc Finger Transcription Factor BbCmr1 Regulates Conidium Maturation in Beauveria bassianaMicrobiology SpectrumFebruary 23, 2022Jin-Feng Chen, Jun-Jie Tan, Jun-Yao Wang, A-Jing Mao, Xue-Ping Xu, Yan Zhang, Xue-li Zheng, Yu Liu, Dan jin, Xian-Bi Li, et al.(IPTG, 0.1 mM) was added to induce protein expression for 20 h at 20°C. Proteins were purified using the MagneGST Protein Purification System (Promega, USA) and verified by SDS–PAGE (Fig. S5). EMSA. For EMSAs, biotin-labeled DNA fragments containing

(7) PBK Enhances Cellular Proliferation With Histone H3 Phosphorylation and Suppresses Migration and Invasion With CDH1 Stabilization in Colorectal CancerFrontiers in PharmacologyJanuary 18, 2022Akira Koshino et al.GST-tagged proteins used in the kinase assays were produced in Escherichia coli and purified by the MagneGST™ Protein Purification System (Promega). In vitro kinase reactions were performed in 1x kinase buffer (Cell Signaling) with adenosine

(8) AKR1A1 Variant Associated With Schizophrenia Causes Exon Skipping, Leading to Loss of Enzymatic ActivityFrontiers in GeneticsDecember 6, 2021Kyoka Iino et al.min. Glutathione-S-transferase (GST) fusion AKR1A1 from BL21 E. coli transformed with pGEX-4T-2_AKR1A1 was purified using the MagneGST Protein Purification System (Promega) according to the manufacturer’s protocols. Protein concentrations were determined using the

(9) A Rice Immunophilin Homolog, OsFKBP12, Is a Negative Regulator of Both Biotic and Abiotic Stress ResponsesInternational Journal of Molecular SciencesNovember 20, 2020Ming-Yan Cheung, Wan-Kin Auyeung, Kwan-Pok Li, Hon-Ming Lamfusion proteins and MBP-tagged fusion proteins were extracted by MagneGSTTM Protein Purification System (V8600; Promega Biosciences, CA, USA) and SpinClean™ MBP Excellose® Spin Kit (23020; Mbiotech, Inc., Haman,

(10) ABAS1 from soybean is a 1R-subtype MYB transcriptional repressor that enhances ABA sensitivityJournal of experimental botanyMay 30, 2020Ku YS, Ni M, Muñoz NB, Xiao Z, Lo AW, Chen P, Li MW, Cheung MY, Xie M, Lam HM.transformed with pGEX-4T-1 alone or pGEX-4T-1-GmABAS1 using the MagneGST™ Protein Purification System (V8600, Promega) according to the manufacturer’s protocol. After protein elution, the protein buffer was changed

(11) A distinct class of plant and animal viral proteins that disrupt mitosis by directly interrupting the mitotic entry switch Wee1-Cdc25-Cdk1Science advancesMay 1, 2020Jin H, Du Z, Zhang Y, Antal J, Xia Z, Wang Y, Gao Y, Zhao X, Han X, Cheng Y, et al.with the GST-17K recombinant protein purified using a MagneGST protein purification system (Promega, V8603). The purified proteins were quantified using the Bradford assay and checked by SDS-PAGE. Cdc25 phosphatase activity assay

(12) Characterization of an MLP Homologue from Haemaphysalis longicornis (Acari: Ixodidae) TicksPathogensApril 14, 2020Jin Luo, Hui Shen, Qiaoyun Ren, Guiquan Guan, Bo Zhao, Hong Yin, Ronggui Chen, Hongying Zhao, Jianxun Luo, Xiangrui Li, et al.1.0 mM) for 8 h at 37 ◦ C and with shaking at 180 rpm. The recombinant rMLP was purified using the MagneGST™ Protein Purification System according to the manufacturer’s instructions (Promega, Madison, WI, USA), and the N- or C-terminal glutathione S-transferase (GST) tag

(13) AMP-activated protein kinase regulates circadian rhythm by affecting CLOCK in DrosophilaThe Journal of NeuroscienceFebruary 28, 2019Eunjoo Cho, Miri Kwon, Jaewon Jung, Doo Hyun Hyun Kang, Sanghee Jin, Sung-E Choi, Yup Kang, Eun Young Kimwere purified from Escherichia coli BL21 cells, which were transformed with pGEX4T-1-dClk, using the MagneGST protein purification system (Promega). Briefly, E. coli pellets were lysed with lysis buffer (MagneGST cell lysis reagent, Promega)

(14) TOX Regulates Growth, DNA Repair, and Genomic Instability in T-cell Acute Lymphoblastic LeukemiaCancer DiscoveryNovember 1, 2017Riadh Lobbardi et al.translated using the 1-Step Human High-Yield mini IVT kit (Pierce). TOX proteins were purified using the MagneGST Protein Purification System (Promega) and interactions with KU70/KU80 assessed on column. Specifically, 0.5μg–3μg of KU70/KU80 proteins

(15) Mechanical Control of Whole Body Shape by a Single Cuticular Protein Obstructor-E in Drosophila melanogasterPLOS GeneticsJanuary 11, 2017Reiko Tajiri, Nobuhiro Ogawa, Haruhiko Fujiwara, Tetsuya Kojimaaccording to the manufacturer’s instruction. Protein purification using glutathione beads was done using MagneGST Protein Purification System (Promega). Purified proteins were desalted using Microcon Centrifugal Filters (Merck Millipore), and

(16) ATP binding by the P-loop NTPase OsYchF1 (an unconventional G protein) contributes to biotic but not abiotic stress responsesProceedings of the National Academy of SciencesMarch 8, 2016Ming-Yan Cheung, Xiaorong Li, Rui Miao, Yu-Hang Fong, Kwan-Pok Li, Yuk-Lin Yung, Mei-Hui Yu, Kam-Bo Wong, Zhongzhou Chen, Hon-Ming Lamcultivating at 25 °C overnight. The protein was purified by using the MagneGST Protein Purification System (V8603; Promega). Nucleotide-binding assays were performed as described (30). One hundred nanomolar 2′/3′-O-(N-methyl-anthraniloyl)

(17) A Course-Based Undergraduate Research Experience Investigating p300 Bromodomain Mutations#Biochemistry and Molecular Biology EducationJanuary 2, 2016Erin K. Shanle, Ian K. Tsun, Brian D. StrahlSupporting Information File 2 (Week 3 and 4). In brief, protein purification was performed using the MagneGST protein purification system (Promega) according to the manufacturer's protocol with slight modifications. Briefly, pellets were

(18) The Photosystem II Oxygen-Evolving Complex Protein PsbP Interacts With the Coat Protein of Alfalfa mosaic virus and Inhibits Virus ReplicationMolecular Plant-Microbe InteractionsÂ®October 1, 2014Muthukumar Balasubramaniam, Bong-Suk Kim, Heather M. Hutchens-Williams, L. Sue Loesch-Friesco-immunoprecipitation assay. The pull-down assays with GST fusion proteins were performed with the MagneGST protein purification system (Promega Corp.) as per the manufacturer’s instructions. Briefly, pGEX-encoded GST protein or pGEX-CP-encoded GST-CP

(19) Expression of a Toll Signaling Regulator Serpin in a Mycoinsecticide for Increased VirulenceApplied and Environmental MicrobiologyAugust 1, 2014Linzhi Yang, Nemat O. Keyhani, Guirong Tang, Chuang Tian, Ruipeng Lu, Xin Wang, Yan Pei, Yanhua Fandesignated the crude extract. Soluble GST-Spn43Ac protein in the crude extract was purified using a MagneGST protein purification system (Promega). Glutathione S-transferase (GST) from the pGEX-6p-1 empty vector was also purified using the

(20) Evaluation of a disintegrin-like and metalloprotease with thrombospondin type 1 repeat motifs 13 (ADAMTS13) activity enzyme-linked immunosorbent assay for measuring plasma ADAMTS13 activity in dogsJournal of Veterinary Diagnostic InvestigationMarch 4, 2014Haruhiko Maruyama, Michiko Kaneko, Taiga Otake, Rui Kano, Yoshiki Yamaya, Toshihiro Watari, Atsuhiko Hasegawa, Hiroshi KamataSt. Louis, MO. His Mag Sepharose Ni, GE Healthcare, Little Chalfont, Buckinghamshire, United Kingdom. MagneGST protein purification system, Promega Corp., Madison, WI. Amicon Ultra-10k, EMD Millipore Corp., Billerica, MA. DC Protein Assay

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