Description
KAPA Library Amplification Kits contain KAPA HiFi DNA Polymerase, a novel enzyme engineered for ultra-high fidelity and robustness using Kapa’s directed evolution technology platform.
KAPA HiFi has become the enzyme of choice for NGS library amplification due to its ability to amplify complex DNA populations with high fidelity, high efficiency, decreased PCR duplication rates and very low bias. This results in lower duplication rates and improved coverage of GC- and AT rich regions, promoters, low complexity and other challenging regions in all NGS library construction workflows requiring library amplification.
In addition to the standard library amplification formulation, KAPA HiFi is available in a unique real-time formulation, for applications that demand precise control over library amplification. A uracil-tolerant variant, KAPA HiFi Uracil+ is also available for the high-efficiency, high-fidelity, low-bias amplification of libraries constructed from bisulfite-treated DNA.
KAPA Library Amplification Kits for Illumina platforms now also include an optimally formulated Library Amplification Primer Mix.
Kits include KAPA HiFi HotStart ReadyMix (2X), a convenient PCR master mix containing KAPA HiFi HotStart DNA Polymerase, KAPA dNTPs, reaction buffer, and MgCl2 at a final concentration of 2.5 mM. Kits with primers are also available. Kits for real-time library amplification include a real-time version of the KAPA HiFi HotStart ReadyMix, as well as fluorescent standards to monitor library amplification.