SNAP-Surface 549 from New England Biolabs

Product Specs
ItemSNAP-Surface 549
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberS9112S
Quantity50 nmol
ApplicationsCellular Biology
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Simulating the conformational dynamics of the ATPase complex on proteasome using its free-energy landscapeSTAR ProtocolsDecember 1, 2023Rui Fang, Ying Luethanol,Decon Labs,Cat. # V1001 Pure hexane,Fisher Scientific,Cat. # H302 SNAP-surface 549,NEB,Cat. # S9112S Streptavidin,Invitrogen,Cat. # 434302 Tween-20,Sigma-Aldrich,Cat. # P1379 TEV protease,Invitrogen,Cat.

(2) Architecture of androgen receptor pathways amplifying glucagon-like peptide-1 insulinotropic action in male pancreatic β cellsCell reportsMay 30, 2023Xu W, Qadir MMF, Nasteska D, Mota de Sa P, Gorvin CM, Blandino-Rosano M, Evans CR, Ho T, Potapenko E, Veluthakal R, et al.Ingelheim,N/A Finasteride,Sigma Aldrich,Cat# F1293 Dutasteride,Sigma Aldrich,Cat# SML1221 SNAP-Surface 549,NEB,Cat# S9112S Exendin4,Sigma Aldrich,Cat# E7144 Exendin-Phe1,Imperial College London,N/A Streptozotocin,Sigma Aldrich,Cat#

(3) SIM imaging resolves endocytosis of SARS-CoV-2 spike RBD in living cellsCell chemical biologyMarch 16, 2023Miao L, Yan C, Chen Y, Zhou W, Zhou X, Qiao Q, Xu Z.293-TI cell culture supplement,Sino Biological,Cat # M293-SUPI SNAP-Cy3,New England BioLabs,Cat # S9112S SNAP-Alexa 488,New England BioLabs,Cat # S9129S Lyso561,KeyGEN BioTECH,Cat # KGMP006 Bafilomycin A1

(4) Identification of transient intermediates during spliceosome activation by single molecule fluorescence microscopyProceedings of the National Academy of SciencesNovember 29, 2022Xingyang Fu, Harpreet Kaur, Margaret L. Rodgers, Eric J. Montemayor, Samuel E. Butcher, Aaron A. HoskinsSNAP-tagged proteins were fluorophore labeled as previously described (48). Briefly, SNAP-Surface® 549 dye (S9112S, New England BioLabs; abbreviated elsewhere as SNAP-DY-549 or DY-549) in DMSO was added to 1.2 mL yWCE

(5) Aberrant Cholesterol Metabolism and Wnt/β-Catenin Signaling Coalesce via Frizzled5 in Supporting Cancer GrowthAdvanced science (Weinheim, Baden-Wurttemberg, Germany)October 1, 2022Zheng S, Lin J, Pang Z, Zhang H, Wang Y, Ma L, Zhang H, Zhang X, Chen M, Zhang X, et al.sodium,Sigma‐Aldrich,P4498 Methyl‐β‐cyclodextrin,Sigma‐Aldrich,332615 Sulfo‐NHS‐SS‐Biotin,APExBIO,A8005 SNAP‐Surface 549,New England Biolabs (NEB),#S9112S SNAP‐Cell Oregon Green,New England Biolabs (NEB),#S9104S Cycloheximide,Sigma‐Aldrich,5087390001 2‐Bromohexadecanoic

(6) A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteinsG3 (Bethesda, Md.)September 30, 2022Baek I, Le SN, Jeon J, Chun Y, Reed C, Buratowski S.Tfa2-HA3-SNAP). SNAP fusions in the extracts were labeled with SNAP-Surface 549 (New England Biolabs, cat# S9112S) during nuclear extract preparation. Extracts were then resolved by 10% SDS-PAGE. Center panel shows

(7) Conformational fingerprinting of allosteric modulators in metabotropic glutamate receptor 2eLifeJuly 1, 2022Brandon Wey-Hung Liauw, Arash Foroutan, Michael R Schamber, Weifeng Lu, Hamid Samareh Afsari, Reza Vafabakhshdrug",MNI-137,Tocris,"Cat # 4,388", "Chemical compound, drug",SNAP-Surface Alexa Fluor 549,New England Biolabs,S9112S, "Chemical compound, drug",SNAP-Surface Alexa Fluor 647,New England Biolabs,S9136S, "Chemical compound,

(8) An empirical energy landscape reveals mechanism of proteasome in polypeptide translocationeLifeJanuary 20, 2022Rui Fang, Jason Hon, Mengying Zhou, Ying Ludrug",Methotrexate,Sigma-Aldrich,A6770, "Chemical compound, drug",Biliverdin,Sigma-Aldrich,30891, "Chemical compound, drug",SNAP-surface-549,NEB,S9112S, "Strain, strain background (Escherichia coli)",NiCo21 DE3,NEB,C2529H, "Software, algorithm",MATLAB

(9) Single-molecule studies reveal branched pathways for activator-dependent assembly of RNA polymerase II pre-initiation complexesMolecular CellSeptember 1, 2021Inwha Baek, Larry J. Friedman, Jeff Gelles, Stephen Buratowskifusion proteins in nuclear extracts were labeled by adding SNAP-Surface 549 (New England BioLabs, #S9112S) to a final concentration of 0.4 μM (unless otherwise specified) and incubating at 4°C for 1 hr on a

(10) Calponin-homology domain mediated bending of membrane-associated actin filamentseLifeJuly 16, 2021Saravanan Palani, Sayantika Ghosh, Esther Ivorra-Molla, Scott Clarke, Andrejus Suchenko, Mohan K Balasubramanian, Darius Vasco Köster7.5, and 1 mM DTT), flash frozen in liquid N2 and stored at −80°C. SNAP labeling (SNAP-Surface 549, S9112S, NEB) of capping protein-beta and Rng2 1–189 was performed as per the manufactures protocol. SDS-PAGE Purity

(11) Antagonistic roles for Ataxin-2 structured and disordered domains in RNP condensationeLifeMarch 10, 2021Singh A, Hulsmeier J, Kandi AR, Pothapragada SS, Hillebrand J, Petrauskas A, Agrawal K, Rt K, Thiagarajan D, Jayaprakashappa D, et al.,Vectashield Mounting Medium,Vector Laboratories,Cat# H-1000, Chemical compound,SNAP-Surface 549,New England Biolabs,Cat# S9112S,IF (1:500) "Software, algorithm",TRIBE,"McMahon et al., 2016",https://github.com/rosbashlab/TRIBE, "Software,

(12) A Non-amyloid Prion Particle that Activates a Heritable Gene Expression ProgramMolecular CellJanuary 1, 2020Anupam K. Chakravarty, Tina Smejkal, Alan K. Itakura, David M. Garcia, Daniel F. Jaroszmiscellaneous resources" Chemicals,Chemicals,Chemicals SNAP-Surface® 549,New England Biolabs,Cat# S9112S SNAP-Surface® 488,New England Biolabs,Cat# S9124S Poly ethylene glycol (PEG) - MW 8000,Millipore Sigma,Cat#

(13) PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 PathwaysImmunityDecember 17, 2019Zhao Y, Lee CK, Lin CH, Gassen RB, Xu X, Huang Z, Xiao C, Bonorino C, Lu LF, Bui JD, et al.S9234S SNAP-Surface Alexa Fluor 647,New England Biolabs,Cat # S9136S SNAP-Surface 549,New England Biolabs,Cat # S9112S SNAP-Cell 505-Star,New England Biolabs,Cat # S9103S SNAP-Cell TMR-Star,New England Biolabs,Cat # S9105S SNAP-Cell 647-SiR

(14) Supported Cell Membrane Sheets to Monitor Protein AssemblyBIO-PROTOCOLSeptember 20, 2019Simon Erlendsson, Thor Seneca Thorsen, Kenneth Lindegaard MadsenThermoFisher, catalog number: A20000) 37. *SNAP-surface dye 568/647 (New England Biolabs, catalog numbers: S9112S, S9137) 38. D-MEM (Gibco, Sigma-Aldrich, catalog number: 11965-092) 39. Fetal Bovine Serum (Gibco, catalog

(15) Mechanisms of PDZ domain scaffold assembly illuminated by use of supported cell membrane sheetseLifeJanuary 3, 2019Simon Erlendsson, Thor Seneca Thorsen, Georges Vauquelin, Ina Ammendrup-Johnsen, Volker Wirth, Karen L Martinez, Kaare Teilum, Ulrik Gether, Kenneth Lindegaard Madsen(Sigma),, Other,Alexa Fluor C5 malemide 488/568,"A10254, A20341 (ThermoFisher)",, Other,SNAP-surface dye 568/647,"S9112S, S9137 (New England Biolabs)",, Other,Alexa Fluor 488 (NHS),A20000 (ThermoFisher),, Other,SuperSignal ELISA Femto Substrate

(16) Synergistic assembly of human pre-spliceosomes across introns and exonseLifeJune 22, 2018Braun JE, Friedman LJ, Gelles J, Moore MJ.centrifuging for 10 min at 12,000 × g, the SNAP-Surface 549 dye-benzylguanine conjugate (New England BioLabs, S9112S) was added to the supernatant at a final concentration of 200 nM and incubated for 30 min at 30°C. After

(17) Second-Generation Drosophila Chemical Tags: Sensitivity, Versatility, and SpeedGeneticsApril 1, 2017Ben Sutcliffe, Julian Ng, Thomas O. Auer, Mathias Pasche, Richard Benton, Gregory S. X. E. Jefferis, Sebastian Cachero,New England Biolabs,S9102S SNAP-Surface 549 (SNAP-549),Dyomics DY-549P1,560,575,SNAPm/f,No,New England Biolabs,S9112S CLIP-Surface 488 (CLIP-488),ATTO-TEC 488,506,526,CLIPm/f,No,New England Biolabs,S9232S CLIP-Surface 547

(18) A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expressionGenome biologyNovember 10, 2015Xi L, Schmidt JC, Zaug AJ, Ascarrunz DR, Cech TR.ImageQuant TL v2005 software. To detect the SNAP-tag, 10 μM SNAP Surface® 594 (New England Biolabs, S9112S) was added to the input samples at the beginning of the IP. The fluorescence signals were detected with

(19) Dynamic caveolae exclude bulk membrane proteins and are required for sorting of excess glycosphingolipidsNature CommunicationsNovember 1, 2015Elena Shvets, Vassilis Bitsikas, Gillian Howard, Carsten Gram Hansen, Benjamin J. Nichols647-conjugated human transferrin (Invitrogen, T23366); SNAP Cell surface Reagent 549 (New England Biolabs, S9112S); cyclohexamide (Santa-Cruz, sc-3508); okadaic acid (Santa-Cruz, sc-95060);and Magic Red Cathepsin L

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SNAP-Surface 549 from New England Biolabs

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New England Biolabs

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SNAP-Surface 549 from New England Biolabs

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