SpectraMax® Paradigm® Multi-Mode Microplate Detection Platform from Molecular Devices LLC

Product Specs
ItemSpectraMax® Paradigm® Multi-Mode Microplate Detection Platform
CompanyMolecular Devices LLC
Price Get Pricing Get Quote for this Product
DetectionFluorescence / Fluorescence Polarization / Time Resolved Fluorescence / Luminescence
Optical SystemMonochromator
Plate Formats6 - 1536 well plates
Temperature Range4 - 45 °C
Wavelength(s)230 - 1000 nm
Light SourceHigh Powered LED / Flash Lamp
ApplicationsAbsorbance, ELISA, Fluorescence, FP, Luminescence, , Western blotting, HTRF, Kinase/Phosphatase Assays, AlphaScreen, TRF, TR-FRET & HTRF

Specifications/Features

Shaking, Temperature control

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Molecular Devices LLC

3860 N First Street

San Jose, California 95134

United States

Phone: 408-747-1700

Company Profile

Website: http://www.moleculardevices.com/

(1) Vertical impedance electrode array for spatiotemporal dynamics monitoring of 3D cells under drug diffusion effectiScienceDecember 15, 2023Yong Qiu, Xin Liu, Yuxuan Zhu, Deming Jiang, Fengheng Li, Weijie Yu, Hao Wan, Liujing Zhuang, Yuxiang Pan, Ping Wang96-well plates and the fluorescence intensity of each well was measured under the microplate reader (SpectraMax Paradigm, Molecular Devices, USA). Optimization of detection conditions is accomplished through a built-in program.

(2) Structural Basis of μ-Opioid Receptor-Targeting by a Nanobody AntagonistbioRxivDecember 7, 2023Jun Yu, Amit Kumar, Xuefeng Zhang, Charlotte Martin, Pierre Raia, Antoine Koehl, Toon Laeremans, Jan Steyaert, Aashish Manglik, Steven Ballet, et al.triplicates. Upon reaching equilibrium after 3 h, the FRET signal at 620 nm and 660 nm was read using a SpectraMax Paradigm Multi-Mode Microplate Reader (Molecular Devices). The ratio of the acceptor and donor emission signals

(3) MicroRNAs in Small Extracellular Vesicles from Amniotic Fluid and Maternal Plasma Associated with Fetal Palate Development in MiceInt J Mol SciDecember 6, 2023Xige Zhao, Xia Peng, Zhiwei Wang, Xiaoyu Zheng, Xiaotong Wang, Yijia Wang, Jing Chen, Dong Yuan, Ying Liu, Juan Du24, 48, and 72 h, followed by incubation at 37 °C for 2 h. Each sample was analyzed at 450 nm with a SpectraMax Paradigm microplate reader (10822-512, Molecular Devices, San Jose, CA, USA) [62]. 4.7. Apoptosis Assay Approximately

(4) Melatonin suppresses Akt/mTOR/S6K activity, induces cell apoptosis, and synergistically inhibits cell growth with sunitinib in renal carcinoma cells via reversing Warburg effectRedox report : communications in free radical researchDecember 1, 2023Xue KH, Jiang YF, Bai JY, Zhang DZ, Chen YH, Ma JB, Zhu ZJ, Wang X, Guo P.for 10 min. The ATP content in cell lysates and mitochondrial suspensions were then measured using a SpectraMax Paradigm multi-mode microplate reader (Molecular Devices). A portion of the tumor tissue was added to 150 μL

(5) Mutant p53R211* ameliorates inflammatory arthritis in AIA rats via inhibition of TBK1-IRF3 innate immune responseInflammation ResearchDecember 1, 2023Yaling Zeng, Jerome P. L. Ng, Linna Wang, Xiongfei Xu, Betty Yuen Kwan Law, Guobing Chen, Hang Hong Lo, Lijun Yang, Jiujie Yang, Lei Zhang, et al.(MEIMIAN, China) according to manufacturer’s instruction. The absorbance was measured at 450 nm using SpectraMax Paradigm (Molecular Devices, USA). Nuclear and cytoplasmic extraction The transfected cells were harvested

(6) Metformin Suppresses Stemness of Non-Small-Cell Lung Cancer Induced by Paclitaxel through FOXO3aInternational Journal of Molecular SciencesNovember 22, 2023Zhimin Tang, Yilan Zhang, Zhengyi Yu, Zhijun Luothe culture and incubated for 2 h. Absorbance at 450 nm was measured using an auto-microplate reader (SpectraMax Paradigm Molecular Devices, Silicon Valley, CA, USA). The percentage of inhibition was calculated using the following

(7) CDC7 inhibition induces replication stress-mediated aneuploid cells with an inflammatory phenotype sensitizing tumors to immune checkpoint blockadeNature CommunicationsNovember 18, 2023Tomoko Yamamori Morita, Jie Yu, Yukie Kashima, Ryo Kamata, Gaku Yamamoto, Tatsunori Minamide, Chiaki Mashima, Miyuki Yoshiya, Yuta Sakae, Toyohiro Yamauchi, et al.The supernatant was then incubated at 37 °C for 2 h, and the optical density was read at 650 nm in SpectraMax Paradigm (Molecular devices). Luciferase activity was measured using the QUANTI-Luc (InvivoGen, #rep-qlc). The

(8) Low Glycolysis Is Neuroprotective during Anoxic Spreading Depolarization (SD) and Reoxygenation in LocustseNeuroNovember 6, 2023Yuyang Wang (王宇扬), Alexander G. Little, Maria J. Aristizabal, R. Meldrum Robertson(Sigma-Aldrich; catalog #QPBCA). All colorimetric and fluorometric assays were performed using the SpectraMax Paradigm microplate reader (Molecular Devices). Caspase-3 activity assay The level of neural caspase three

(9) Berberine and aspirin prevent traumatic heterotopic ossification by inhibition of BMP signalling pathway and osteogenic differentiationJournal of cellular and molecular medicineNovember 1, 2023Fan J, Gao J, Chen J, Hou J, Liu M, Dang Y, Lin H.Biotechnology). After 1 h, the OD value of each well was measured at 450 nm using a micro‐plate reader (SpectraMax Paradigm Multi‐Mode Detection Platform, Molecular Devices). 2.4 Alkaline phosphatase (ALP) staining and Alizarin

(10) Cistanche deserticola Polysaccharide Reduces Inflammation and Aging Phenotypes in the Dermal Fibroblasts through the Activation of the NRF2/HO-1 PathwayInternational Journal of Molecular SciencesOctober 28, 2023Kento Takaya, Toru Asou, Kazuo Kishiwere washed with Hanks’ balanced salt solution, and the fluorescence intensity was measured using a SpectraMax Paradigm (Molecular Devices, San Jose, CA, USA) using excitation at: 490–520 nm/emission: 510–540 nm. LDH levels

(11) β-Caryophyllene Inhibits Oxaliplatin-Induced Peripheral Neuropathy in Mice: Role of Cannabinoid Type 2 Receptors, Oxidative Stress and NeuroinflammationAntioxidants (Basel)October 22, 2023Jonathan Paulo Agnes, Barbara dos Santos, Raquel Nascimento das Neves, Vitória Maria Marques Luciano, Larissa Benvenutti, Fernanda Capitanio Goldoni, Roberta Giusti Schran, José Roberto Santin, Nara Lins Meira Quintão, Alfeu Zanotto-Filhocentrifuged to remove debris, and the fluorescence was read at 485/532 nm (excitation/emission) using a Spectramax Paradigm microplate reader (Molecular Devices, San Jose, CA, USA). The data were expressed as DCF fluorescence

(12) Non-Steroidal Estrogens Inhibit Influenza Virus by Interacting with Hemagglutinin and Preventing Viral FusionInternational Journal of Molecular SciencesOctober 19, 2023Elisa Franzi, Gregory Mathez, Soraya Dinant, Charlotte Deloizy, Laurent Kaiser, Caroline Tapparel, Ronan Le Goffic, Valeria Cagnowas added to the cells and blocked with 1N Chloridric Acid. The absorbance was read at 450 nm with SpectraMAx Paradigm (Molecular Devices, San Jose, CA, USA). The percentage of infection was calculated by subtracting the

(13) Synthesis and evaluation of a novel PET ligand, a GSK’963 analog, aiming at autoradiography and imaging of the receptor interacting protein kinase 1 in the brainEJNMMI Radiopharmacy and ChemistryOctober 18, 2023Hiroshi Ikenuma, Aya Ogata, Hiroko Koyama, Bin Ji, Hideki Ishii, Takashi Yamada, Junichiro Abe, Chie Seki, Yuji Nagai, Masanori Ichise, et al.incubated for 45 min at room temperature under shading. Luminescence was measured with a microplate reader (SpectraMax® Paradigm®, MOLECULAR DEVICES). Curve fitting was performed using GraphPad Prism® sigmoidal dose–response software

(14) Effect of mRNA-LNP components of two globally-marketed COVID-19 vaccines on efficacy and stabilitynpj VaccinesOctober 11, 2023Lizhou Zhang, Kunal R. More, Amrita Ojha, Cody B. Jackson, Brian D. Quinlan, Hao Li, Wenhui He, Michael Farzan, Norbert Pardi, Hyeryun Choefollowed by addition of 100 μl 1× Ribogreen dye to each well to bind RNA. Fluorescence was measured by SpectraMax Paradigm Multi-Mode Detection Platform (Molecular Devices) at 485 nm excitation and 528 nm emission. mRNA encapsulation

(15) Improve BBB Penetration and Cytotoxicity of Palbociclib in U87-MG Glioblastoma Cells Delivered by Dual Peptide Functionalized NanoparticlesPharmaceuticsOctober 6, 2023Yu-Chen Lo, Wen-Jen Linthe formazan crystals. The absorbance was measured at 570 nm and 690 nm using a microplate reader (SpectraMax Paradigm, Molecular Devices, San Jose, CA, USA), and the cell viability (%) was calculated using equation 3.

(16) Investigation and Evaluation of the Transdermal Delivery of Ibuprofen in Various Characterized Nano-Drug Delivery SystemsPharmaceuticsOctober 3, 2023Jeanri Myburgh, Wilna Liebenberg, Clarissa Willers, Admire Dube, Minja Gerberon a microplate shaker for 1 h to solubilize the MTT-formazan crystals. The plates were placed in a SpectraMax® Paradigm® multi-mode microplate reader (Molecular devices, San Jose, CA, USA) to measure the absorbance with

(17) Structure–Activity Relationships of Low Molecular Weight Alginate Oligosaccharide Therapy against Pseudomonas aeruginosaBiomoleculesSeptember 8, 2023Manon F. Pritchard, Lydia C. Powell, Jennifer Y. M. Adams, Georgina Menzies, Saira Khan, Anne Tøndervik, Håvard Sletta, Olav Aarstad, Gudmund Skjåk-Bræk, Stephen McKenna, et al.temperature in the dark for 5 min, mixed briefly, and luminescence measured on a Molecular Devices SpectraMax Paradigm Multi-Mode Detection Platform. The MSD was calculated using the Tukey–Kramer method using Minitab 17.2.1

(18) Zonula occludens-1 distribution and barrier functions are affected by epithelial proliferation and turnover ratesCell proliferationSeptember 1, 2023Imafuku K, Iwata H, Natsuga K, Okumura M, Kobayashi Y, Kitahata H, Kubo A, Nagayama M, Ujiie H.1 h. The tracer fluorescence intensities of the apical and bottom chambers were measured using the SpectraMax Paradigm Multi‐Mode Microplate Reader (Molecular Devices, San Jose, CA). The ratio of tracer fluorescence intensities

(19) Nitric Oxide Detection Using a Chemical Trap Method for Applications in Bacterial Systems †MicroorganismsAugust 31, 2023Marilene Silva Oliveira, Karina F. D. N. Santos, Railane Monteiro de Paula, Luciana C. Vitorino, Layara A. Bessa, Alexander Greer, Paolo Di Mascio, João C. P. de Souza, Claudia C. G. Martin-Didonetthe supernatant was deposited in a 96-well ELISA plate (suitable for fluorescence) and analyzed using SpectraMax Paradigm Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) with excitation and emission at

(20) Specific inhibition of an anticancer target, polo-like kinase 1, by allosterically dismantling its mechanism of substrate recognitionProceedings of the National Academy of SciencesAugust 29, 2023Jung-Eun Park, Klara Kirsch, Hobin Lee, Paola Oliva, Jong Il Ahn, Harsha Ravishankar, Yan Zeng, Stephen D. Fox, Samuel A. Kirby, Pooja Badhwar, et al.concentrations. All the samples were analyzed approximately 30 min after reaction in a 384-well format using the SpectraMax Paradigm multimode microplate detection platform (Molecular Devices). Data were analyzed after carrying out at

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