BD Accuri™ C6 Plus Flow Cytometer from BD Biosciences

Product Specs
ItemBD Accuri™ C6 Plus Flow Cytometer
CompanyBD Biosciences
Price Get Pricing Get Quote for this Product
Catalog NumberBD Accuri C6 Plus
QuantityEA
Abs / Exc488 nm (Blue), 640 nm (Red)
DetectionThe system is equipped with a blue laser and a red laser, and capable of 6 detection parameters; (including FSC and SSC). Two scatter detectors and four fluorescence detectors with interference filters optimized for the detection of FITC, PE, PerCP-Cy™5.5 and APC.
Sample TypeStandard 96-well plates (flat, round and v-bottom) Deep-well 96-well plates 48-well plates. Plates manufactured to the guidelines published by the American National Standards Institute, submitted by the Society for Biomolecular Screening, should be compatible with the BD CSampler Plus. 12 x 75-mm tubes can also be accommodated using the supplied 24-tube rack.
SensitivityFluorescence sensitivity

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Website: http://www.bdbiosciences.com/

(1) Upregulated LAMA3 modulates proliferation, adhesion, migration and epithelial‑to‑mesenchymal transition of cholangiocarcinoma cellsSci RepDecember 18, 2023Kittiya Islam, Brinda Balasubramanian, Simran Venkatraman, Parichut Thummarati, Janpen Tunganuntarat, Nut Phueakphud, Phongthon Kanjanasirirat, Tanawadee Khumpanied, Pornparn Kongpracha, Yingpinyapat Kittirat, et al.Scientific) and then incubated at room temperature for 5 min in the dark. Cells (104) were collected using BD Accuri C6 Plus flow cytometer (BD Biosciences) and the percentage of apoptotic cells was acquired from an addition of the percentage

(2) Strain and temperature dependent aggregation of Candida auris is attenuated by inhibition of surface amyloid proteinsThe Cell SurfaceDecember 15, 2023Dhara Malavia-Jones, Rhys A. Farrer, Mark H.T. Stappers, Matt B. Edmondson, Andrew M. Borman, Elizabeth M. Johnson, Peter N. Lipke, Neil A.R. Gowrpm. Following incubation, yeast cell suspensions were vortexed and 30,000 events were analysed using BD Accuri C6 Plus flow cytometer (BD Biosciences, Sydney) – and gated as in (Silva-Dias et al., 2012). For amyloid exposure, C. auris

(3) Frentizole, a Nontoxic Immunosuppressive Drug, and Its Analogs Display Antitumor Activity via Tubulin InhibitionInt J Mol SciDecember 14, 2023Sergio Ramos, Alba Vicente-Blázquez, Marta López-Rubio, Laura Gallego-Yerga, Raquel Álvarez, Rafael Peláez50 μg/mL propidium iodide (Sigma-Aldrich, St. Louis, MO, USA), and Triton 10× at room temperature. A BD Accuri™ C6 Plus Flow Cytometer (BD Bioscience, Madrid, Spain) was used to analyze samples, and BD Accuri™ C6 Software (version 1.0.264.21)

(4) Forsythiaside A Activates AMP-Activated Protein Kinase and Regulates Oxidative Stress via Nrf2 SignalingInt J Mol SciDecember 1, 2023Young Eun Kim, Seon-Been Bak, Min-Jin Kim, Su-Jin Bae, Won-Yung Lee, Young Woo Kimcells were trypsinized and centrifuged at 4 °C for 3 min at 3000 rpm. Cell MMP was detected using a BD Accuri™ C6 Plus flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA) [5,7]. 4.6. FACS Analysis of Annexin V and PI Staining HepG2 cells

(5) Resistomycin Inhibits Wnt/β-Catenin Signaling to Induce the Apoptotic Death of Human Colorectal Cancer CellsMarine DrugsNovember 29, 2023Yaoyao Zhu, E Zhang, Huan Gao, Chuangeng Shang, Mengxiong Yin, Mingtao Ma, Yu Liu, Xuanfeng Zhang, Xia Liapoptosis detection kit (Bestbio, Shanghai, China) as per the provided directions, followed by analysis using a BD Accuri™ C6 Plus flow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA). 4.6. Immunofluorescence The cells were plated on glass

(6) Resolvin D1 promotes the resolution of inflammation in the ACLF rat model by increasing the proportion of Treg cellsImmunity, Inflammation and DiseaseNovember 20, 2023Linjun Chen, Yixuan Huang, Yizhen Chen, Jiaxuan Chen, Xueye You, Laiyu Zou, Jiabing Chen, Zhixin Chen, Xiaozhong Wang, Yuehong Huang30 min at room temperature to mark the Treg cells. The detection of Treg cells was performed using a BD Accuri C6 Plus flow cytometer (BD). 2.6 Real‐time polymerase chain reaction (RT‐PCR) Total RNA of rat liver was extracted by RNA

(7) Infection pressure in apes has driven selection for CD4 alleles that resist lentivirus (HIV/SIV) infectionbioRxivNovember 18, 2023Cody J. Warren, Arturo Barbachano-Guerrero, Vanessa L. Bauer, Alex C. Stabell, Obaiah Dirasantha, Qing Yang, Sara L. SawyerBD Biosciences 560635) and AlexaFluor647 mouse anti-human CD4 (BD Biosciences, 566681), and analyzed using a BD Accuri C6 Plus flow cytometer (BD Biosciences). HIV/SIV Envelope clones used in this study. Envelope clones for HIV-1 and SIVcpz

(8) A Macrophages-Enriched Head and Neck Tumor Spheroid Model to Study Foslip® Behavior in Tumor MicroenvironmentInternational Journal of NanomedicineNovember 9, 2023Aurélie Francois, Luca Dirheimer, Alicia Chateau, Henri-Pierre Lassalle, Ilya Yakavets, Lina BezdetnayaSSC-A characteristics-gated events to identify cell populations. Flow cytometry was performed using a BD Accuri C6 Plus flow cytometer (BD Biosciences, Erembodegem, Belgium). Polarization of Macrophages Recombinant human IL-4, IL-10,

(9) Green tea extract improves cyclophosphamide-induced immunosuppression in mouse spleen and enhances the immune activity of RAW 264.7 cellsHeliyonNovember 8, 2023Jeong-Won Kim, Jin-Hwa Kim, Chang-Yeop Kim, Ji-Soo Jeong, Je-Won Ko, Tae-Won Kimmin at 4 °C in the dark. After washing the cells twice, FACS data were acquired and analyzed using a BD Accuri C6 Plus flow cytometer (BD Biosciences). 2.2.5 Histopathological examination A portion of formalin-fixed spleen was processed,

(10) Tarin-Loaded Nanoliposomes Activate Apoptosis and Autophagy and Inhibit the Migration of Human Mammary Adenocarcinoma CellsInternational Journal of NanomedicineNovember 7, 2023Raiane Vieira Cardoso, Patricia Ribeiro Pereira, Cyntia Silva Freitas, Érika Bertozzi de Aquino Mattos, Anna Victoria De Freitas De Freitas Silva, Victor do Valle Midlej, Mauricio Afonso Vericimo, Carlos Adam Conte-Júnior, Vania Margaret Flosi Paschoalindark according to the manufacturer’s instructions. Cells were then analyzed by flow cytometry using a BD Accuri C6 plus Flow Cytometer (BD Bioscience) and fluorescence as detected at 502/530 nm absorption/emission wavelengths (FL-1). Statistical

(11) Loss of ADAR1 in macrophages in combination with interferon gamma suppresses tumor growth by remodeling the tumor microenvironmentJournal for ImmunoTherapy of CancerNovember 7, 2023Weiwei Lin, Yikai Luo, Jie Wu, Haowan Zhang, Ge Jin, Chahua Guo, Hang Zhou, Han Liang, Xiaoyan Xuthen stained for intracellular targets using specific antibodies. Stained cells were acquired using a BD Accuri C6 Plus Flow Cytometer, and FlowJo was used to analyze data. CD8+ T cells chemotactic assay We added 600 µL RPMI 1640 containing

(12) Impaired mitophagosome–lysosome fusion mediates olanzapine‐induced agingAging CellNovember 1, 2023Xi Chen, Zhizhen Wang, Peng Zheng, Anjila Dongol, Yuanyi Xie, Xing Ge, Mingxuan Zheng, Xuemei Dang, Zehra Boz Seyhan, Nathan Nagaratnam, et al.level, respectively. After being washed with PBS three times, cells were collected and analyzed by BD Accuri™ C6 Plus flow cytometer (BD Biosciences) and FlowJo software (BD). 4.11 Immunofluorescence assay The immunofluorescence assay

(13) The mutations on the envelope glycoprotein D contribute to the enhanced neurotropism of the pseudorabies virus variantJournal of Biological ChemistryNovember 1, 2023Hongxia Wu, Hansong Qi, Bing Wang, Mingzhi Li, Liang Qu, Su Li, Yuzi Luo, Lian-Feng Li, Guang-Lai Zheng, Hua-Ji Qiu, et al.goat anti-murine IgG (H+L) Alexa Fluor 488 for 45 min at 4 °C. A total of 104 cells were analyzed by a BD Accuri C6 Plus Flow Cytometer (BD Bioscience), and the data were analyzed using the FlowJo software version 10 (TreeStar). Puriﬁcation

(14) The biological effects of terahertz wave radiation-induced injury on neural stem cellsiScienceOctober 20, 2023Yunxia Wang, Yu Xiong, Man Chen, Fei Liu, Haiyan He, Qinlong Ma, Peng Gao, Guiming Xiang, Liqun Zhang9.0.0,GraphPad,https://www.graphpad.com/ ,, Other,Other,Other ,, Leica DMI8 fluorescence microscope,Leica,Leica DMI8 BD Accuri C6 plus flow cytometer,BD,BD Accuri C6 plus

(15) Pectic homogalacturonan sensed by Bacillus acts as host associated cue to promote establishment and persistence in the rhizosphereiScienceOctober 20, 2023Farah Boubsi, Grégory Hoff, Anthony Arguelles Arias, Sébastien Steels, Sofija Andrić, Adrien Anckaert, Romain Roulard, Augustin Rigolet, Olivier van Wuytswinkel, Marc Ongenahomogenizer,Bandelin,https://profilab24.com/en/laboratory/ultrasonic/bandelin-sonopuls-hd-2070-homogeniser BD Accuri™ C6 Plus Flow cytometer,BD Biosciences,https://www.bdbiosciences.com/en-us/products/instruments/flow-cytometers/research-cell-analyzers/bd-accuri-c6-plus Nikon

(16) Besnoitia besnoiti-induced neutrophil clustering and neutrophil extracellular trap formation depend on P2X1 purinergic receptor signalingFrontiers in ImmunologyOctober 3, 2023Gabriel Espinosa, Iván Conejeros, Lisbeth Rojas-Barón, Carlos Rodrigo Hermosilla, Anja Taubertapoptosis detection kit (ab14085, Abcam) following the manufacturer´s instructions and analyzed with a BD Accuri C6 Plus Flow Cytometer (BD, USA). FlowJo v10.8.1 software was used to determine the apoptosis/necrosis rate. 2.10 Statistical

(17) Targeting human mitochondrial NAD(P)+-dependent malic enzyme (ME2) impairs energy metabolism and redox state and exhibits antileukemic activity in acute myeloid leukemiaCell Oncol (Dordr)October 1, 2023Kun-Chi Chen, I-Hsin Hsiao, Yu-Nan Huang, Yu-Tung Chou, Yi-Chun Lin, Ju-Yi Hsieh, Yung-Lung Chang, Kang-Hsi Wu, Guang-Yaw Liu, Hui-Chih Hungwashed with PBS buffer and subjected to fluorescence-activated cell sorter (FACS) analysis. Using a BD Accuri C6 Plus flow cytometer, data was collected (BD Biosciences, San Jose, CA, USA). Live cell fluorescence microscopy ATP was

(18) Functional inhibition of the RNA‐binding protein HuR sensitizes triple‐negative breast cancer to chemotherapyMolecular OncologyOctober 1, 2023Lanjing Wei, Qi Zhang, Cuncong Zhong, Lily He, Yuxia Zhang, Ahlam M. Armaly, Jeffrey Aubé, Danny R. Welch, Liang Xu, Xiaoqing WuRNAse) and incubated for 30 min at room temperature in the dark. Stained cells were analyzed by the BD Accuri C6 Plus flow cytometer (BD Science). A total of 1 × 105 events (FL2 > 80 000) for each sample were counted and calculated by

(19) Ex Vivo Colonic Fermentation of NUTRIOSE® Exerts Immuno-Modulatory Properties and Strong Anti-Inflammatory EffectsNutrientsSeptember 30, 2023Caroline Perreau, Clementine Thabuis, Lynn Verstrepen, Jonas Ghyselinck, Massimo Marzoratiparticular sequences in their DNA. Total cell counts in the various samples were determined using a BD Accuri C6 Plus Flow Cytometer (BD Biosciences, Franklin Lakes, NJ, USA) using the high flow rate setting with a threshold of 700 on

(20) The PML1-WDR5 axis regulates H3K4me3 marks and promotes stemness of estrogen receptor-positive breast cancerResearch SquareSeptember 9, 2023Hung-Ying Kao, Chun-Peng Pai, Han Wang, Neel Agarwal, Joshua Adams, Zhenghao Liu, Darcie Seachrist, Ruth Keri, William SchiemannALDEFLUOR assay was performed following the manufacturer’s instructions, followed by flow cytometry using a BD Accuri C6 Plus Flow Cytometer (BD Biosciences) with the electronic gating set according to cells stained with the corresponding Control

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BD Accuri™ C6 Plus Flow Cytometer from BD Biosciences

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BD Accuri™ C6 Plus Flow Cytometer from BD Biosciences

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