MACSQuant® Analyzer 16 Flow Cytometer from Miltenyi Biotec

Product Specs
ItemMACSQuant® Analyzer 16 Flow Cytometer
CompanyMiltenyi Biotec
Price Get Pricing Get Quote for this Product
Catalog Number130-109-803
QuantityEA
Abs / Exc405 nm (violet), 488 nm (blue), 640 nm (red)
DetectionFSC, SSC, 14 fluorescence channels
Volume1µL min. measurement volume (10 µL dead volume)
ApplicationsFlow Cytometry
ThroughputUp to 15,000 events per second
Dimensions(WxDxH) 669 x 400 x 394-553.5 mm (adjustable touch screen)
Measuring Time< 25 minutes per 96-well plate (5 µL measurement volume per well)
Flow Rate25, 50, or 100 µL/min or automate flow rate to maintain 500, 1000, or 2000 events/second
SoftwareMACSQuantify, integrated acquisition and analysis software
Format1.5/2.0/5.0/15/50 mL tubes or 24/48/96-well plates

Add to Compare List

Local Office

Miltenyi Biotec

Miltenyi Biotec Inc.

2303 Lindbergh Street

Auburn, CA 95602

United States

Phone: Phone: +1 530 888 8871
Toll Free: 800 FOR MACS
Fax: +1 530 888 8925

Email: macs@miltenyibiotec.com

Company Profile

Website: www.miltenyibiotec.com

International Office

Miltenyi Biotec

Miltenyi Biotec B.V. & Co. KG

Friedrich-Ebert-Straße 68

51429 Bergisch Gladbach

Germany

Phone: +49 2204 8306-0
Fax: +49 2204 8306-235

Email: macs@miltenyibiotec.de

Company Profile

Website: www.miltenyibiotec.com

(1) Isolation methods determine human neutrophil responses after stimulationFrontiers in ImmunologyNovember 24, 2023Vanessa Krémer, Ophélie Godon, Pierre Bruhns, Friederike Jönsson, Luc de Chaisemartinfor 15 min. The cells were washed and resuspended in MACS buffer (Miltenyi Biotec) and aquired on a MACSQuant 16 Analyzer (Miltenyi Biotec), prior to analysis of data using FlowJo 10.6.2. Analysis of purity, quantity

(2) Plasmacytoid Dendritic Cell, Slan+-Monocyte and Natural Killer Cell Counts Function as Blood Cell-Based Biomarkers for Predicting Responses to Immune Checkpoint Inhibitor Monotherapy in Non-Small Cell Lung Cancer PatientsCancers (Basel)November 3, 2023Francesca Pettinella, Chiara Lattanzi, Marta Donini, Elena Caveggion, Olivia Marini, Giulia Iannoto, Sara Costa, Elena Zenaro, Tiago Moderno Fortunato, Sara Gasperini, et al.were acquired using an eight-color three-laser MACSQuant 10 Analyzer or a fourteen-color three-laser MACSQuant 16 Analyzer (Miltenyi Biotec, Bergisch Gladbach, Germany) while the data analysis was performed using the

(3) The slan antigen identifies the prototypical non-classical CD16+-monocytes in human bloodFrontiers in ImmunologyOctober 27, 2023Nicola Tamassia, Francisco Bianchetto-Aguilera, Sara Gasperini, Alessio Grimaldi, Claudia Montaldo, Federica Calzetti, Elisa Gardiman, Ilaria Signoretto, Monica Castellucci, Vincenzo Barnaba, et al.APC (REA491, Miltenyi Biotec) for 30 min at RT. Sample fluorescence was then measured by a 14-color MACSQuant16 Analyzer (Miltenyi Biotec) flow cytometer, while data analysis was performed using FlowJo software version

(4) Comparative Immune Response after Vaccination with SOBERANA® 02 and SOBERANA® plus Heterologous Scheme and Natural Infection in Young ChildrenVaccines (Basel)October 25, 2023Rocmira Pérez-Nicado, Chiara Massa, Laura Marta Rodríguez-Noda, Anja Müller, Rinaldo Puga-Gómez, Yariset Ricardo-Delgado, Beatriz Paredes-Moreno, Meiby Rodríguez-González, Marylé García-Ferrer, Ilianet Palmero-Álvarez, et al.Gladbach, Germany, 130-128-032), following the manufacturer’s instructions. Data were acquired on a MACSQuant16 Flow Cytometer (Miltenyi-Biotec) and analyzed by FlowJo software X 10.0.7 (FlowJo LLC, Ashland, OR,

(5) Discovery of neutralizing SARS-CoV-2 antibodies enriched in a unique antigen specific B cell clusterPLOS ONESeptember 20, 2023Stine Sofie Frank Lende, Nanna Møller Barnkob, Randi Westh Hansen, Harsh Bansia, Mike Vestergaard, Frederik Holm Rothemejer, Anne Worsaae, Deijona Brown, Maria Lange Pedersen, Anna Halling Folkmar Rahimic, et al.in 1% PFA for at least 15 min at 4°C, prior to analysis of cell eGFP expression on a Miltenyi Biotec MACSquant16 flow cytometry. In vivo studies Animal experiments were conducted under animal license 2020-15-0201-00726

(6) SARS-CoV-2 specific plasma cells acquire long-lived phenotypes in human bone marrowEBioMedicineAugust 8, 2023Axel R. Schulz, Leonard Fiebig, Heike Hirseland, Lisa-Marie Diekmann, Simon Reinke, Sebastian Hardt, Antonia Niedobitek, Henrik E. Meiazide), pelleted at 700×g, for 7 min, at 4 °C, resuspended in 2 mL PBA, and counted volumetrically on a MACSQuant 16 (Miltenyi Biotech). Up to 15 × 106 cells were transferred to a FACS-Tube (Sarstedt), pelleted and resuspended

(7) Oral prednisolone suppresses skin inflammation in a healthy volunteer imiquimod challenge modelFrontiers in ImmunologyJuly 20, 2023Salma Assil, Thomas P. Buters, Pieter W. Hameeteman, Charlie Hallard, Nicoline Treijtel, Tessa Niemeyer – Van der Kolk, Marieke L. de Kam, Edwin F. I. I. I. Florencia, Errol P. Prens, Martijn B. A. van Doorn, et al.cells and incubated for 30 min on ice. The stained samples were washed with PBS and measured using MACSQuant 16 (Miltenyi Biotec GmbH). Flow cytometry data were analyzed with Flowlogic 7.2 (Inivai). Parallel to the

(8) Protective immunity induced by an inhaled SARS-CoV-2 subunit vaccineVaccineJuly 19, 2023Elizabeth Elder, Chandrashekar Bangalore Revanna, Catharina Johansson, Robert P.A. Wallin, Johan Sjödahl, Ola Winqvist, Ali Mirazimi(ThermoFisher Scientific) according to the manufacturer’s instructions. The samples was analyzed with a MACSQuant16 instrument (Miltenyi Biotech). 3.9 SARS-CoV-2 RT-qPCR Equal volumes of BAL from each mouse was mixed

(9) Identification of peripheral vascular function measures and circulating biomarkers of mitochondrial function in patients with mitochondrial diseaseClinical and Translational ScienceJuly 1, 2023Sebastiaan J. W. van Kraaij, Diana R. Pereira, Bastiaan Smal, Luciana Summo, Anne Konkel, Janine Lossie, Andreas Busjahn, Tom N. Grammatopoulos, Erica Klaassen, Robert Fischer, et al.After staining, PBMCs were washed twice with phosphate buffer saline and analyzed by flow cytometry (MACSQuant16, Miltenyi Biotec). For flow cytometry data, to ensure proper gate setting, a minimum of 100,000 events

(10) The RIG-I agonist M8 triggers cell death and natural killer cell activation in human papillomavirus-associated cancer and potentiates cisplatin cytotoxicityCancer Immunology, ImmunotherapyJune 25, 2023Carlo Girone, Federica Calati, Irene Lo Cigno, Valentina Salvi, Valentina Tassinari, Tiziana Schioppa, Cinzia Borgogna, Ludovica Lospinoso Severini, John Hiscott, Cristina Cerboni, et al.F4/80+ (macrophages), CD3+CD4+ and CD3+C8+ (T cells) and NK1.1+CD3− (NK cells). Cells were analyzed on a MACSQuant16 (Miltenyi) and analyzed with FlowJo software (RRID:SCR_008520, Treestar) [28]. Quantitative PCR RNA

(11) Plasmodium falciparum Gametes and Sporozoites Hijack Plasmin and Factor H To Evade Host Complement KillingMicrobiology spectrumJune 15, 2023Ernest M, Rosa TFA, Pala ZR, Kudyba HM, Sweeney B, Reiss T, Pradel G, Vega-Rodríguez J.with propidium iodide (1 μg/mL; Thermo Fisher Scientific) for 15 min and analyzed using the Miltenyi MACSQuant 16 flow cytometer. Single-stain controls were used for compensation. Enzyme-linked immunosorbent assay. To

(12) Increased retention of functional mitochondria in mature sickle red blood cells is associated with increased sickling tendency, hemolysis and oxidative stressHaematologicaJune 1, 2023Sofia Esperti, Elie Nader, Antoine Stier, Camille Boisson, Romain Carin, Muriel Marano, Mélanie Robert, Marie Martin, Françoise Horand, Agnes Cibiel, et al.and Mitotracker Red CMXRos Dye (Invitrogen) were used to label active mitochondria. Flow cytometry (MACSQuant 16, Miltenyi), MitoTracker(R) Deep Red probe (MTKdr, Sigma-aldrich) and anti-CD71 antibody were used to

(13) Immune Monitoring of Mycophenolate Mofetil Activity in Healthy Volunteers Using Ex Vivo T Cell Function AssaysPharmaceuticsMay 31, 2023Aliede E. in ’t Veld, Manon A. A. Jansen, Marieke L. de Kam, Yalҫin Yavuz, Dirk Jan A. R. Moes, Kathalijne A. Oudhoff, Mariette I. E. van Poelgeest, Jacobus Burggraaf, Matthijs Moerlandway as in our previous study [17] using an EdU kit (Thermo Fisher Scientific, (Waltham, MA, USA). A MACSQuant 16 analyzer (Miltenyi Biotec, Bergisch Gladbach, Germany) was used for flow cytometry analysis of EdU incorporation.

(14) Continuous freeze-drying of messenger RNA lipid nanoparticles enables storage at higher temperaturesJournal of controlled release : official journal of the Controlled Release SocietyMay 1, 2023Meulewaeter S, Nuytten G, Cheng MHY, De Smedt SC, Cullis PR, De Beer T, Lentacker I, Verbeke R.stained to identify dead cells using a 7-AAD live/dead stain (ThermoFisher) and were analyzed using the MACSQuant 16 flow cytometer (Miltenyi Biotec) to quantify eGFP-expression. 2.6 Cryo-electron microscopy mRNA LNPs

(15) Impact of age and comorbidities on SARS-CoV-2 vaccine-induced T cell immunityCommunications MedicineApril 24, 2023Lisa Loksø Dietz, Anna Karina Juhl, Ole Schmeltz Søgaard, Joanne Reekie, Henrik Nielsen, Isik Somuncu Johansen, Thomas Benfield, Lothar Wiese, Nina Breinholt Stærke, Tomas Østergaard Jensen, et al.P0DTC2) of SARS-CoV-2 (Wuhan-Hu-1 lineage). Following stimulation, cells were washed, stained and run on MACSQuant16 (Miltenyi Biotec). Data was analyzed using FlowJo™ v10.8.1 Software (BD Biosciences). For viability

(16) Nanobody‐mediated complement activation to kill HIV‐infected cellsEMBO Molecular MedicineApril 11, 2023Maria Lange Pedersen, Dennis Vestergaard Pedersen, Mikael Becher Lykkegaard Winkler, Heidi Gytz Olesen, Ole Schmeltz Søgaard, Lars Østergaard, Nick Stub Laursen, Anna Halling Folkmar Rahimic, Martin Tolstrupcurve using the GraphPad Prism software. Data processing and statistical analysis The Miltenyi Biotec MACSQuant 16 flow cytometer was used to acquire data by flow cytometry. Data were analyzed using FlowJo (version

(17) The Relationship between PBLS and Osteosarcoma Distribution in Different Subgroups and the Survival and Prognosis of OsteosarcomaJournal of OncologyApril 7, 2023Peng Luo, Peng ZhouCD3+ (%), CD3+CD4+ (%), and CD3+CD8+ (%) in IPBs suspension cells were detected using flow cytometry (130-109-803, Miltenyi Biotec, Germany). Meanwhile, the ratios of CD4+/CD8+, CD4+/CD3+, and CD8+/CD3+ in each group

(18) Immunosuppression by hydroxychloroquine: mechanistic proof in in vitro experiments but limited systemic activity in a randomized placebo-controlled clinical pharmacology studyImmunologic ResearchFebruary 22, 2023Aliede E. in ‘t Veld, Hendrika W. Grievink, Johan L. van der Plas, Boukje C. Eveleens Maarse, Sebastiaan J. W. van Kraaij, Tess D. Woutman, Mascha Schoonakker, Naomi B. Klarenbeek, Marieke L. de Kam, Ingrid M. C. Kamerling, et al.antibodies and propidium iodide as viability dye (all Miltenyi Biotec, Bergisch-Gladbach, Germany) using a MACSQuant 16 analyzer (Miltenyi Biotec). After 24 h, culture supernatants were collected for cytokine analysis. PBMC

(19) The Effects of Interstitial Lung Diseases on Alveolar Extracellular Vesicles Profile: A Multicenter StudyInternational Journal of Molecular SciencesFebruary 17, 2023Miriana d’Alessandro, Sara Gangi, Piera Soccio, Elisabet Cantó, Rubén Osuna-Gómez, Laura Bergantini, Paolo Cameli, Gaia Fabbri, Sara Croce, Giulia Scioscia, et al.Cassina De’ Pecchi–Milano, Italy), while EVs isolated at Barcelona Hospital were characterized using a MACSQuant Analyzer 16 Flow Cytometer (Miltenyi Biotec, Bergisch Gladbach, Germany). According to literature data, the results from the two

(20) The immunoregulatory effect of the TREM2-agonist Sulfavant A in human allogeneic mixed lymphocyte reactionFrontiers in ImmunologyFebruary 14, 2023Giusi Barra, Carmela Gallo, Dalila Carbone, Marcello Ziaco, Mario Dell’Isola, Mario Affuso, Emiliano Manzo, Genoveffa Nuzzo, Laura Fioretto, Giuliana D’Ippolito, et al.CFSE negative cells. Flow cytometry Flow cytometry was performed by FACSAriaTM (BD Bioscience) or MACSQUANT 16® Analyzer (Miltenyi Biotec). For surface staining, cells were washed in PBS and stained with Viobility™

Be the First to Write a Review!

Related Products

Item	Catalog Number	Get Quote
MACSQuantify™ Software	MACSQuantify	Get Pricing