IMPACT KIT from New England Biolabs

The IMPACT™ (Intein Mediated Purification with an Affinity Chitin-binding Tag) Kit utilizes engineered protein splicing elements (termed inteins) to purify recombinant proteins by a single column. This kit distinguishes itself from other protein fusion systems by its ability to separate a recombinant protein from the affinity tag without the use of a protease.

• Single-column purification without the use of proteases to remove the affinity tag
• Able to produce target protein without vector–derived amino acids
• Fusion to either C-terminus or N-terminus of target protein
• Isolation of proteins with or without an N-terminal methionine residue
• Ligation and labeling of recombinant proteins