Exonuclease III (E.coli) from New England Biolabs

Product Specs
ItemExonuclease III (E.coli)
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0206L
Quantity25000 units
Concentration100u/µl
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeEnzymes
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to remove 200 base pairs from each end of linearized double-stranded phiX174 DNA (650 µg/ml) in 10 minutes at 30°C.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Targeted Phasing of 2-200 Kilobase DNA Fragments with a Short-Read Sequencer and a Single-Tube Linked-Read Library MethodbioRxivMarch 10, 2023Veronika Mikhaylova, Madison Rzepka, Tetsuya Kawamura, Yu Xia, Peter L. Chang, Shiguo Zhou, Long Pham, Naisarg Modi, Likun Yao, Adrian Perez-Agustin, et al.then digested with 40 U of Exonuclease I (Cat. #X8010; Enzymatics®), 100 U of Exonuclease III (Cat. #M0206L; New England Biolabs), and 20 U of Lambda exonuclease (Cat. #M0262S; New England Biolabs) simultaneously

(2) RIOK3 promotes mTORC1 activation by facilitating SLC7A2-mediated arginine uptake in pancreatic ductal adenocarcinomaAgingFebruary 24, 2023Qin H, Sun R, Guo X, Fang L, Xu M, Teng Y, Zhen N, Wang A, Liu J.vectors which were cut by XhoI and BamHI. The expression vectors were produced utilizing Exonuclease III (M0206L, NEB, USA). Transfection was performed with Lipofectamine 2000 (29223-1-AP 1:1000, Proteintech, China). Lentiviral

(3) A reference human induced pluripotent stem cell line for large-scale collaborative studiesCell stem cellDecember 1, 2022Pantazis CB, Yang A, Lara E, McDonough JA, Blauwendraat C, Peng L, Oguro H, Kanaujiya J, Zou J, Sebesta D, et al.Fisher Scientific,14190-144 Hoechst 33258,Millipore Sigma,94403 Exonuclease III,New England Biolabs,M0206L Essential 8 medium,Thermo Fisher Scientific,A1517001 Y-27632,Selleck Chem,S1049 Doxorubicin,Bio-Techne,2252 Puromycin,Millipore Sigma

(4) On-microscope staging of live cells reveals changes in the dynamics of transcriptional bursting during differentiationNat CommunNovember 4, 2022D. M. Jeziorska, E. A. J. Tunnacliffe, J. M. Brown, H. Ayyub, J. Sloane-Stanley, J. A. Sharpe, B. C. Lagerholm, C. Babbs, A. J. H. Smith, V. J. Buckle, et al.incorporation during DNA replication. Cells were fixed and permeabilised before using exonuclease III (M0206L, NEB) digestion to enable resection of a single DNA strand after treatment with Hoechst 33258 and 254

(5) Pathogenic variants in SLF2 and SMC5 cause segmented chromosomes and mosaic variegated hyperploidyNature communicationsNovember 4, 2022Grange LJ, Reynolds JJ, Ullah F, Isidor B, Shearer RF, Latypova X, Baxley RM, Oliver AW, Ganesh A, Cooke SL, et al.33258 (Sigma-Aldrich, 861405) in 2x SSC for 15 min at RT, UV-irradiated, and digested with ExoIII (NEB M0206L) for at least 30 min at 37 °C. Slides were then washed once in 1x PBS and dehydrated in an ethanol series

(6) Reconfiguration of DNA nanostructures induced by enzymatic ligation treatmentNucleic Acids ResearchAugust 12, 2022Tanxi Bai et al.T4 polynucleotide kinase (Catalog #M0201L), T4 ligase (Catalog #M0202L), exonuclease III (Catalog #M0206L). DNA sequence design Structures shown in this study were adapted from previous studies (26,27), including

(7) Rapid Detection of Actinobacillus pleuropneumoniae From Clinical Samples Using Recombinase Polymerase AmplificationFrontiers in Veterinary ScienceMarch 25, 2022Oliver W. Stringer et al.Technologies, Risskov, Denmark, Cat #RPA-BF-2), 2 U/μL Escherichia coli Exonuclease III (NEB, Hitchin, UK, Cat #M0206L), 14 mM MgAc, and 1 μL of DNA template, in a total of 5 μL/reaction for limit of detection and specificity

(8) Efficient acquisition of tens of thousands of short tandem repeats in single-cell whole-genome-amplified DNASTAR ProtocolsDecember 17, 2021Liming Tao, Zipora Marx, Ofir Raz, Ehud ShapiroBuffer,Epicentre,Cat#A3210K Exonuclease I (E. coli),NEB,Cat#M0293L Exonuclease III (E. coli),NEB,Cat#M0206L RecJf,NEB,Cat#M0264L Exonuclease T,NEB,Cat#M0265L T7 Exonuclease,NEB,Cat#M0263L Lambda Exonuclease,NEB,Cat#M0262L NEBNext

(9) ATRX proximal protein associations boast roles beyond histone depositionPLoS geneticsNovember 1, 2021Scott WA, Dhanji EZ, Dyakov BJA, Dreseris ES, Asa JS, Grange LJ, Mirceta M, Pearson CE, Stewart GS, Gingras AC, et al.exposed to 365 nm UV, 5.4 x 103 J/m2 twice. Next, slides were treated with 10 U/μL exonuclease III (NEB, M0206L) for 40 min at 37°C. Cells were washed in 1X PBS and then dehydrated in a series of 70, 95, and 100%

(10) Genome profiles of pathologist-defined cell clusters by multiregional LCM and G&T-seq in one triple-negative breast cancer patientCell Reports MedicineOctober 19, 2021Zhongyi Zhu et al.(600U/μL),Enzymatics,Cat# L6030 Exonuclease I (20U/μL),NEB,Cat# M0293L Exonuclease III (100U/μL),NEB,Cat# M0206L dNTPs (2.5mM),Invitrogen,Cat# R72501 BSA,NEB,Cat# B9000S rTaq (5U/μL),Thermo Fisher,Cat# EP0402 Phosphate

(11) Large-scale preparation of fluorescence multiplex host cell reactivation (FM-HCR) reportersNature ProtocolsSeptember 1, 2021C. G. Piett, T. J. Pecen, D. J. Laverty, Z. D. Nagelcat. no. B7203S) NEBuffer™4 (NEB, cat. no. B7004S). Exonucleases: Exonuclease III (NEB, cat. no. M0206L) T5 Exonuclease (NEB, cat. no. M0363L) Dimethyl sulfoxide (DMSO; VWR, cat. no. BDH1115-1LP) Ethanol,

(12) Retrospective cell lineage reconstruction in humans by using short tandem repeatsCell Reports MethodsJuly 26, 2021Liming Tao et al.Buffer,EPICENTRE,Cat#A3210K Exonuclease I (E.coli),NEB,Cat#M0293L Exonuclease III (E.coli),NEB,Cat#M0206L RecJf,NEB,Cat#M0264L Exonuclease T,NEB,Cat#M0265L T7 Exonuclease,NEB,Cat#M0263L Lambda Exonuclease,NEB,Cat#M0262L NEBNext

(13) Telomere Length Dynamics and Chromosomal Instability for Predicting Individual Radiosensitivity and Risk via Machine LearningJournal of Personalized MedicineMarch 8, 2021Jared J. Luxton, Miles J. McKenna, Aidan M. Lewis, Lynn E. Taylor, Sameer G. Jhavar, Gregory P. Swanson, Susan M. BaileyCrosslinker (365 nm UV), and digested with exonuclease III (New England Biolabs, Ipswich, NA, USA; #M0206L) for 30 min. Paint hybridization mixture was applied to slides, which were then coverslipped, sealed

(14) Novel CRISPR-based sequence specific enrichment methods for target loci and single base mutationsPLOS ONEDecember 23, 2020Jennifer L. Steele, Richard C. Stevens, Oscar A. Cabrera, Gary J. Bassill, Sabrina M. Cramer, Felipe Guzman, Anthony P. Shuberthen mixed with target DNA and incubated for 60 minutes at 37°C. Next, exonuclease III (NEB, cat. #M0206L) and exonuclease VII (NEB, cat. #M0379L) were added with NEBuffer 1 (NEB, cat. #B7001S) and incubated

(15) Cohesin Disrupts Polycomb-Dependent Chromosome Interactions in Embryonic Stem CellsCell ReportsJanuary 21, 2020James D.P. Rhodes et al.53022 Digoxygenin-11-dUTP,Roche,Cat# 1073963 HEPES,GIBCO,Cat# 15630056 DAPI,Roche,Cat# 10236276001 Exonuclease III,NEB,Cat# M0206L Kreatech Hybridization Buffer,Leica,Cat# KBI-FHB T4 DNA ligase,Thermo Fisher Scientific,Cat# EL0012 T4 DNA polymerase

(16) A CO-FISH assay to assess sister chromatid segregation patterns in mitosis of mouse embryonic stem cellsChromosome ResearchJanuary 8, 2020Stephan Sauer, Sandra S. Burkett, Mark Lewandoski, Amar J. S. KlarExonuclease III treatment). This was followed by 25 min of Exonuclease III treatment at 37 °C (NEB M0206L, 1,000 U/slide in 100 μl volume), and two washes in 2× SSC. Next, chromosomal DNA was denatured by transferring

(17) A novel CRISPR/Cas9 associated technology for sequence-specific nucleic acid enrichmentPLOS ONEApril 18, 2019Richard C. Stevens, Jennifer L. Steele, William R. Glover, Jorge F. Sanchez-Garcia, Stephen D. Simpson, Devon O’Rourke, Jordan S. Ramsdell, Matthew D. MacManes, W. Kelley Thomas, Anthony P. Shuberthen mixed with target DNA and incubated for 60 minutes at 37˚C. Next, exonuclease III (NEB, cat. #M0206L) and exonuclease VII (NEB, cat. #M0379S) were added with NEB buffer 1 (NEB, cat. #B7001S) and incubated for

(18) A simple bead-based method for generating cost-effective co-barcoded sequence readsProtocol ExchangeOctober 11, 2018Brock Peters et al.(New England Biolabs, cat. no. M0293L) Exonuclease III \(100,000 units/mL) \(New England Biolabs, cat. no. M0206L) Formamide \(100%, 250 mL) \(Sigma-Aldrich, cat. no. 47671-250ML-F) Glycerol \(100%) \(Sigma-Aldrich,

(19) Multiple serine transposase dimers assemble the transposon-end synaptic complex during IS607-family transpositioneLifeOctober 5, 2018Wenyang Chen, Sridhar Mandali, Stephen P Hancock, Pramod Kumar, Michael Collazo, Duilio Cascio, Reid C Johnsonnumber: EN0521, "Peptide, recombinant protein",Exonuclease III,"NEB, Ipswich, MA",Catalog number: M0206L, "Peptide, recombinant protein",Proteinase K,"Roche, Germany",Catalog number: 03115828001, "Peptide,

(20) Resolving genomic disorder–associated breakpoints within segmental DNA duplications using massively parallel sequencingNature ProtocolsJune 1, 2014Xander Nuttle, Andy Itsara, Jay Shendure, Evan E. EichlerExonuclease I (New England BioLabs, cat. no. M0293L) Exonuclease III (New England BioLabs, cat. no. M0206L) 2X iProof PCR master mix (Bio Rad, cat. no. 172-5311) Primer SLXA_PE_MIPBC_FOR (100 μM, Supplementary

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