Fig 2: A Formalin fixed cholecystectomy specimen with lumens exposed. Conjoined gallbladders 1 and 2 (left and center) showed typical tan velvety biliary type mucosa, whereas attached mass lesion (right) notable for luminal gastric features; pale coarse rugal folds. B Hematoxylin and eosin (H&E) stained microscopic section (20X magnification) demonstrating back-to-back walls of conjoined gallbladders 1 and 2 with typical biliary type mucosa. Mural thickening is present indicating chronic cholecystitis. C Vast majority of separately attached lesion lined by oxyntic (body/fundal) type gastric mucosa (H&E, 100X magnification). Microscopic images obtained using Olympus® BX43 microscope with Olympus® DP27 camera and Olympus® cellSens Entry 2.3 (Build 18,987) image processing software

Fig 3: Beclin-1–dependent autophagy limits the progress of pulmonary pathology in pneumonia-induced sepsis. Mice of WT and Becn1 F121A/F121A were given septic infection by K. pneumoniae (3X107 CFU/mouse, intubation) or PBS in the uninfected group. In parallel groups, infected and uninfected WT mice were treated with TB-peptide (16.5 mg/kg, i.p.) or PBS vehicle 1 h post-infection. Lung tissue was harvested 48 h post-infection, and tissue slides were stained with Hematoxylin and Eosin (H&E). (A) H & E images of lung histology were analyzed under Olympus BX43 microscope at magnifications of 20X and 400X. Images are representative of n=5 per group. (B) Areas of inflammatory infiltration, alveolar congestion, alveolar thickness, and hemorrhage were quantified as percentage of total area using Image J All values are means ± SEM. Significant differences are shown as * for uninfected vs. infected, ** for WT vs. Becn1 F121A/F121A, and *** for TB-peptide treated vs. untreated (p < 0.05, n = 5, student t-test).

Fig 4: Determining CTC by ICC×2 in endometrial and ovarian cancers: CTCs were captured from blood samples from patients with endometrial (A) and ovarian (B) tumors and enumerated using ICC×2 (Ai,Bi). Blood samples were spiked (Spiked samples) with titrating number (250 cells/100 cells) of NCI-H441 cells separately for ICC×2. Corresponding CTC enumeration by IF×3 (Aii,Bii) is presented. For IF×3, pictures were taken at 60× oil objective of an Olympus IX71 Microscope with DAPI/FITC/TRITC/CY5 filter sets. For ICC×2, pictures were taken at 40× objective of an Olympus BX43 Microscope. The magnification, scale bar, and digital reticle are represented for each photomicrograph. Fluorescence images from DAPI, FITC, TRITC, and Cy5 channels were separated as pictures with a color bar. The fluorescence-photomicrographs presented the diameters (μm) of CTC and a representative WBC and their respective DAPI stained nucleus. The immunocytochemistry-photomicrographs are presented with a scale bar, magnification information, digital reticule, as well as the diameters (μm) of CTC and a representative WBC.

Fig 5: Cysts (a) and trophozoites (b) of Giardia duodenalis in a fecal sample and a duodenum biopsy of patients in Spain, respectively. The cysts were detected in microscopic analysis of wet mount of fecal material fixed with the merthiolate-iodine-formaldehyde solution under an Olympus BX43 using a 100× objective, while the trophozoites were detected by microscopic analysis of hematoxylin-eosin stained tissue section under an Olympus BX43 using a 60× objective. Scale-bars: 10 µm