CFX96 Optical Reaction Module from Bio-Rad

Product Specs
ItemCFX96 Optical Reaction Module
CompanyBio-Rad
Price Get Pricing Get Quote for this Product
Catalog Number1845097
Brand/TrademarkBio-Rad Laboratories, Life Science Group
Quantity1EA
ApplicationsAmplification, PCR, qPCR, Real-Time PCR, Genomics, Cloning, Cycle sequencing, Gene expression studies
DetectionFiltered LEDs and filtered photodiodes
ChannelsSix filters, detects up to five targets per well

Features

Interchangeable modules; Adjustable heated lid

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Engineering E. coli strains using antibiotic-resistance-gene-free plasmidsCell Rep MethodsDecember 18, 2023Matthew B. Amrofell, Sunaina Rengarajan, Steven T. Vo, Erick S. Ramirez Tovar, Larissa LoBello, Gautam Dantas, Tae Seok Moonassay microplates,Falcon,353910 AeraSeal,Excel Scientific,Z721573 Biorad CFX96 qPCR machine,Biorad,1845097 MagAttract PowerSoil DNA Kit,Qiagen,27100 KiCqStart SYBR Green qPCR Ready Mix,Sigma,KCQS00 PCR reaction

(2) Uncovering oligodendrocyte enhancers that control Cnp expressionHuman Molecular GeneticsNovember 17, 2023Chuandong Fan, Hongjoo An, Dongkyeong Kim, Yungki ParkFirst-Strand kit (Invitrogen 11904-018). Quantitative PCR was performed on C1000 Thermal Cycler with CFX96 optical reaction module (Bio-rad). Gapdh was used as a loading control. Each PCR reaction contained 2 μl of cDNA, 5 μl of the

(3) Downregulation of complement factor H attenuates the stemness of MDA‑MB‑231 breast cancer cells via modulation of the ERK and p38 signaling pathwaysOncology LettersOctober 18, 2023Soon Yong Park, Da-Young Eum, Yunho Jin, Chae Young Lee, Jae Woong Shim, Si Ho Choi, Seong-Joon Park, Kyu Heo, Yoo Jin ChoiRR036A; Takara Bio, Inc.), according to the manufacturer's instructions. qPCR was performed using a CFX96 Optical Reaction module (Bio-Rad Laboratories, Inc.) with TB Green Premix (cat. no. RR420A; Takara Bio, Inc.) according to the

(4) PARP-1 improves leukemia outcomes by inducing parthanatos during chemotherapyCell reports. MedicineSeptember 19, 2023Maru B, Messikommer A, Huang L, Seipel K, Kovecses O, Valk PJM, Theocharides APA, Mercier FE, Pabst T, McKeague M, et al.Biosciences,Cat# AAF-1003X Biopectrometer Fluorescence,Eppendorf,N/A CFX96™ Real-Time System,Bio-Rad,Cat# 1845097 C1000 Touch™ Thermal Cycler,Bio-Rad,Cat# 1851196 Amersham™ Imager 600 system,GE Healthcare Life Sciences,N/A

(5) Similar but distinct: The impact of biomechanical forces and culture age on the production, cargo loading, and biological efficacy of human megakaryocytic extracellular vesicles for applications in cell and gene therapiesBioengineering & Translational MedicineSeptember 1, 2023Will Thompson, Eleftherios Terry Papoutsakisthe manufacturer's instructions. PCR for each biological replicate was performed in triplicate. The CFX96 Optical Reaction Module (Bio‐Rad, Hercules, CA) was used to control the PCR reactions. Calculation of miRNA levels employed

(6) FoxO and rotund form a binding complex governing wing polyphenism in planthoppersiScienceJuly 21, 2023Chen SJ, Zhang JL, Ma WJ, Wu HJ, Li Y, Shen XX, Xu HJ.assays ,, ChemiDoc XRS + system,Bio-Rad,Cat#1708265 CFX96 real-time PCR detection system,Bio-Rad,Cat#1845097 Zeiss LSM 800 confocal microscope,Carl Zeiss MicroImaging,Cat#400102-9010 LEICA S8AP0 stereomicroscope,LEICA,Cat#10450703 Tabletop

(7) Blind fish have cells that see lightProceedings of the Royal Society B: Biological SciencesJuly 12, 2023Inga A. Frøland Steindal, Yoshiyuki Yamamoto, David Whitmoreoligo dT primers (all from Invitrogen). RT-qPCR was performed on a C1000 Touch Thermal Cycler with the CFX96 Optical Reaction Module (Bio-Rad) using 2× KAPA SYBR FAST qPCR mix (Kapa Biosystems) in technical triplicates with gene specific-primers,

(8) Two Methods for the Isolation and Cultivation of Porcine Primary Corneal CellsMethods and ProtocolsMay 12, 2023Alice Rocha Teixeira Netto, Marc Dieter Hrusa, Karl-Ulrich Bartz-Schmidt, Sven Schnichels, José Hurst89232-908).Thermal cycler Bio-Rad CFX96™ Real-Time System (Bio-Rad, Feldkirchen, Germany; Cat. No. 1845097).Zeiss Axio Observer (CarlZeiss, Oberkochen, Germany; Cat. No. 491916-0002-000).Zeiss Axio Imager Z1

(9) Specific targeting of plasmids with Argonaute enables genome editingNucleic Acids ResearchMay 8, 2023Daria Esyunina, Anastasiia Okhtienko, Anna Olina, Vladimir Panteleev, Maria Prostova, Alexei A Aravin, Andrey Kulbachinskiyfor oligonucleotide sequences), using qPCRmix-HS SYBR premix (Evrogen) in a C1000 Touch Cycler with CFX96 Optical Reaction Module (Bio-Rad). Oligonucleotides for qPCR (synthetized by Evrogen) were selected by their specificity and

(10) Fanconi anemia-associated chromosomal radial formation is dependent on POLθ-mediated alternative end joiningCell ReportsMay 1, 2023Colette B. Rogers, Rachel E. Kram, Kevin Lin, Chad L. Myers, Alexandra Sobeck, Eric A. Hendrickson, Anja-Katrin Bielinskyproject,https://www.r-project.org/ ,, Other,Other,Other ,, CFX96 Touch Real-Time PCRDetection System,BIO-RAD,Cat # 1845097 Zeiss Cell Observer SD SpinningDisk confocal microscope,Zeiss,N/A Countess Automatic Cell Counter,Invitrogen,Cat

(11) Mycobacterium vaccae NCTC 11659, a Soil-Derived Bacterium with Stress Resilience Properties, Modulates the Proinflammatory Effects of LPS in MacrophagesInternational Journal of Molecular SciencesMarch 8, 2023Evan M. Holbrook, Cristian A. Zambrano, Caelan T. O. Wright, Elizabeth M. Dubé, Jessica R. Stewart, William J. Sanders, Matthew G. Frank, Andrew S. MacDonald, Stefan O. Reber, Christopher A. LowryReal-time RT-PCR was conducted in duplicate using the CFX96 Touch Real-Time PCR Detection System (Cat. No. 1845097, Bio-Rad, Hercules, CA, USA) and SYBR Green master mix (Cat. No. 204145, QIAGEN, Hilden, Germany). All

(12) Amplification and Quantitation of Telomeric Extrachromosomal CirclesBIO-PROTOCOLMarch 5, 2023Nathaniel J. Robinson, William P. Schiemannnumber: 1841100) equipped with CFX96 Optical Reaction Module for Real-Time PCR (Bio-Rad, catalog number: 1845097)] Software CFX Maestro Software for CFX Real-Time PCR Instruments (Bio-Rad; https://www.bio-rad.com/en-us/product/cfx-maestro-software-for-cfx-real-time-pcr-instruments?ID=OKZP7E15)Microsoft

(13) Identifying an oligodendrocyte enhancer that regulates Olig2 expressionHuman Molecular GeneticsFebruary 19, 2023Chuandong Fan, Dongkyeong Kim, Hongjoo An, Yungki ParkFirst-Strand kit (Invitrogen 11904–018). Quantitative PCR was performed on C1000 Touch thermal cycler with CFX96 optical reaction module (Bio-rad). Gapdh was used for a loading control. Each PCR reaction contained 2 μL of cDNA, 5 μL of the

(14) Muc2-dependent microbial colonization of the jejunal mucus layer is diet sensitive and confers local resistance to enteric pathogen infectionCell ReportsFebruary 6, 2023George M.H. Birchenough, Bjoern O. Schroeder, Sinan Sharba, Liisa Arike, Christian V. Recktenwald, Fabiola Puértolas-Balint, Mahadevan V. Subramani, Karl T. Hansson, Bahtiyar Yilmaz, Sara K. Lindén, et al.Scientific,240200 FastPrep-24™,MP Biomedicals,116004500 CFX96 Touch Real-Time PCR Detection System,Bio-Rad,1845097 SpectraMax® M2 Multimode microplate reader,Molecular Devices,https://www.moleculardevices.com/products/microplate-readers/multi-mode-readers/spectramax-m-series-readers KEY

(15) Metabolism-Guided Optimization of Tryptophanol-Derived Isoindolinone p53 ActivatorsPharmaceuticals (Basel)January 19, 2023Valentina Barcherini, Joana B. Loureiro, Ana Sena, Catarina Madeira, Paula Leandro, Lucília Saraiva, Alexandra M. M. Antunes, Maria M. M. Santosfinal concentration. The fluorescence was assessed on a C1000 Touch thermal cycler equipped with a CFX96 optical reaction module (BioRad) and the FRET channel (λexc 450–490 nm and λem 560–580 nm). Temperature was ramped from 20 to

(16) A multi-omics study to characterize the transdifferentiation of human dermal fibroblasts to osteoblast-like cellsFrontiers in Molecular BiosciencesNovember 17, 2022Sandra Pihlström, Kirsi Määttä, Tiina Öhman, Riikka E. Mäkitie, Mira Aronen, Markku Varjosalo, Outi Mäkitie, Minna PekkinenApplied Biosystems, Waltham, MA, United States) and the CFX96 Touch Real-Time PCR Detection System (1845097, BioRad, Berkeley, CA, United States) according to previously described protocol (Bustin, 2000; Adibkia

(17) In vivo characterization of key iridoid biosynthesis pathway genes in catnip (Nepeta cataria)PlantaNovember 1, 2022Lira Palmer, Ling Chuang, Marlen Siegmund, Maritta Kunert, Kotaro Yamamoto, Prashant Sonawane, Sarah E. Oâ€™Connoreach target gene was paired to the housekeeping gene UBI9. This reaction was then placed in a Bio-Rad CFX96 Optical Reaction Module. The \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb}

(18) Paracrine Senescence of Mesenchymal Stromal Cells Involves Inflammatory Cytokines and the NF-κB PathwayCellsOctober 21, 2022Chou LY, Ho CT, Hung SC.Carlsbad, CA, USA). Total RNA was subjected to reverse transcription and then qRT-PCR using SYBR green on CFX96 Optical Reaction Module (Bio-Rad, Hercules, CA, USA). Primer sets used to quantify osteogenic differentiation [26] are listed

(19) Bacillus anthracis strain differentiation based on SNP and VNTR lociVavilov Journal of Genetics and BreedingOctober 9, 2022E. A. Anisimova, N. A. Fakhrutdinov, D. A. Mirgazov, E. A. Dodonova, I. A. Elizarova, M. E. Gorbunova, N. I. Khammadov, L. I. Zainullin, K. A. Osyanin(Evrogen, Russia), ddH2O (up to 15 μl). The qPCR procedure was run on a Real-Time С1000 thermocycler with CFX96 optical reaction module (Bio-Rad, USA) under the following protocol: initial DNA denaturation at 95 °C for 3 minutes followed

(20) Dissecting Stemness in Aggressive Intracranial Meningiomas: Prognostic Role of SOX2 ExpressionInternational Journal of Molecular SciencesOctober 2, 2022Rina Di Bonaventura, Maurizio Martini, Tonia Cenci, Valerio Maria Caccavella, Valeria Barresi, Marco Gessi, Alessio Albanese, Liverana Lauretti, Roberto Pallini, Quintino Giorgio D'Alessandris, et al.SOX2 mRNA and actin mRNA, which were taken as the internal reference gene, was performed using the CFX96 optical reaction module (Biorad). The sequences of the primers were as follows: for SOX2, forward 5′-TACAGCATGTCCTACTCGCAG-3′,

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12/15/2020Real Time PCR: Easy to Use Purnima GuptaWe commonly use real time PCR to determine bacterial load by using a broad-range probe and primers set.