MicroPulser Electroporator from Bio-Rad

Product Specs
ItemMicroPulser Electroporator
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog Number1652100
Dimensions(WxDxH) 29 x 21 x 8 cm
QuantityEA
Cell TypeBacteria, yeast, and other microorganisms
MethodElectroporation

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Eukaryotic RNA-guided endonucleases evolved from a unique clade of bacterial enzymesNucleic Acids ResearchDecember 11, 2023Peter H Yoon, Petr Skopintsev, Honglue Shi, LinXing Chen, Benjamin A Adler, Muntathar Al-Shimary, Rory J Craig, Kenneth J Loi, Evan C DeTurk, Zheng Li, et al.electrocompetent E. coli DH10β competent cells (NEB) using 0.1 mm electroporation cuvettes (Bio-Rad) on a Micropulser electroporator (Bio-Rad). The cells were plated on Luria-Bertani (LB)-Agar plates containing ampicillin (100 μg/ml).

(2) Protocol to identify protein-protein interaction networks in Solanum tuberosum using transient TurboID-based proximity labelingSTAR ProtocolsDecember 1, 2023Li Shi, Tatiana Marti Ferrando, Sergio Landeo Villanueva, Matthieu H.A.J. Joosten, Vivianne G.A.A. Vleeshouwers, Christian W.B. BachemmL,Eppendorf,0030120086 "Gene Pulser/MicroPulser electroporation cuvettes, 0.1 cm gap",Bio-Rad,1652089 MicroPulser electroporator,Bio-Rad,1652100

(3) Systematic characterization of the local evolutionary space available to human PKR and vaccinia virus K3bioRxivNovember 25, 2023Michael J. Chambers, Sophia Scobell, Meru J. Sadhuconcentrated reactions into E. cloni 10G SUPREME electrocompetent cells (Lucigen Cat#60080–2) using a MicroPulser Electroporator (BioRad), with 2 μL of the concentrated reaction combined with 25 μL of competent cells and splitting

(4) Sequencing-based functional assays for classification of BRCA2 variants in mouse ESCsCell Reports MethodsNovember 20, 2023Kajal Biswas, Alexander Y. Mitrophanov, Sounak Sahu, Teresa Sullivan, Eileen Southon, Darryl Nousome, Susan Reid, Sakshi Narula, Julia Smolen, Trisha Sengupta, et al.https://www.adobe.com/products/illustrator.html ,, Other,Other,Other ,, Vac-Man vacuum manifold,Promega,Cat# PR-A7231 Micropulser electroporator,Bio-Rad,Cat# 1652100 Gene Pulser Xcell Electroporation Systems,Bio-Rad,Cat# 1652660

(5) Ceragenins exhibit bactericidal properties that are independent of the ionic strength in the environment mimicking cystic fibrosis sputumFrontiers in MicrobiologyNovember 17, 2023Karol Skłodowski, Łukasz Suprewicz, Sylwia Joanna Chmielewska-Deptuła, Szczepan Kaliniak, Sławomir Okła, Magdalena Zakrzewska, Łukasz Minarowski, Robert Mróz, Tamara Daniluk, Paul B. Savage, et al.pMF230 plasmid (Addgene, Watertown, MA, USA) was electroporated into P. aeruginosa PAO1 DSM 19880 using MicroPulser Electroporator (BioRad, Hercules, CA, USA) according to the procedure described by Choi et al. (2006), and transformants

(6) Deciphering cell wall sensors enabling the construction of robust P. pastoris for single-cell protein productionBiotechnology for Biofuels and BioproductsNovember 17, 2023Le Gao, Jiao Meng, Wuling Dai, Zhaokun Zhang, Haofan Dong, Qianqian Yuan, Wuyuan Zhang, Shuguang Liu, Xin Wuwas linearized by AvrII restriction digestion and then electroporated into P. pastoris X33 using a MicroPulser electroporator (BIO-RAD). Positive clones were selected on YPD plates with 100 mg/L zeocin for 48–96 h at 30 °C. The

(7) Engineering of Salmonella Phages into Novel Antimicrobial TailocinsCellsNovember 16, 2023Cedric Woudstra, Anders Nørgaard Sørensen, Lone Brøndstedrecombination. The cells were then harvested and washed 3 times with water. The cells were electroporated (MicroPulser Electroporator Bio Rad with settings: Ec1 (V = 1.8 kV) for 0.1 cm cuvettes and Ec2 (V = 2.5 kV) for 0.2 cm cuvettes)

(8) Shaping human gut community assembly and butyrate production by controlling the arginine dihydrolase pathwaybioRxivNovember 12, 2023Yiyi Liu, Yu-Yu Cheng, Zhichao Zhou, Eugenio I. Vivas, Matthew F. Warren, Federico E. Rey, Karthik Anantharaman, Ophelia S. Venturellitransferred to 1mm gap electro-cuvette. Cells were electroporated using the the EC1 protocol of BioRad Micropulser Electroporator, then recovered in LB plus appropriate antibiotic for 1 hour. Transformants were selected on LB agar

(9) Mining the Utricularia gibba genome for insulator-like elements for genetic engineeringFrontiers in Plant ScienceNovember 8, 2023Daniel Laspisa, Eudald llla-Berenguer, Sohyun Bang, Robert J. Schmitz, Wayne Parrott, Jason Wallacefollowing differences. Twenty-five µL of electrocompetent LBA4404 cells were electroporated in a Bio-Rad MicroPulser™ electroporator (BioRad Laboratories, Hercules CA, US) according to the manufacturer’s user guide with 1 µL of assembled

(10) CXCL17 binds efficaciously to glycosaminoglycans with the potential to modulate chemokine signalingFrontiers in ImmunologyOctober 24, 2023Sean P. Giblin, Sashini Ranawana, Shyreen Hassibi, Holly L. Birchenough, Kyle T. Mincham, Robert J. Snelgrove, Tomoko Tsuchiya, Shiro Kanegasaki, Douglas Dyer, James E. Pease-electroporation cuvette with 50 μL of 10 mg/mL tRNA, with 1 µg of pcDNA3.1-CXCR1 plasmid at 330 V, 950 µF using a MicroPulser electroporator (Bio-Rad). Expression of CXCR1 was confirmed by flow cytometry as previously described (43), and maintained

(11) A phylogenetic approach to explore the Aspergillus fumigatus conidial surface-associated proteome and its role in pathogenesisResearch SquareOctober 13, 2023Gustavo Goldman, Clara Valero, Camila Pinzan, Patrícia de Castro, Norman van van Rhijn, Kayleigh Earle, Hong Liu, Maria Augusta Horta, Olaf Kniemeyer, Thomas Kruger, et al.phaffii (KM71H) by electroporation (1.5 kV, 25 mF and 200 Ω) in a 0.2 μm cuvette using an electroporator (MicroPulser Electroporator, Bio-Rad). The recombinant strain was selected, cultivated and induced to express the rAsparaginase

(12) Environmental DNA transformation resulted in an active phage in Escherichia coliPLOS ONEOctober 13, 2023Abdulkerim Karaynir, Bülent Bozdoğan, Hanife Salih Doğanelectroporation cuvette and electroporation was processed with Ec2 programme (2.49 kV-1 pulse) by using MicroPulser Electroporator device (Bio-rad laboratories, Inc, USA). A volume of 900 μl of Tryptic Soy Broth (TSB) was added to

(13) Mapping temperature‐sensitive mutations at a genome scale to engineer growth switches in Escherichia coliMolecular Systems BiologyOctober 12, 2023Thorben Schramm, Paul Lubrano, Vanessa Pahl, Amelie Stadelmann, Andreas Verhülsdonk, Hannes Link,Zymo Research,#D4004 NucleoSpin Gel and PCR Clean‑up Kit,Macherey‐Nagel,#740609.250 0.1 cm Gene Pulser Cuvette,BioRad,#165‐2089 Micropulser Electroporator,BioRad,#1652100 Breathe‐Easy,Diversified Biotech,#BEM‐1 96‐well plates,Greiner Bio‐One GmbH,#655185

(14) Engineering multifunctional rhizosphere probiotics using consortia of Bacillus amyloliquefaciens transposon insertion mutantseLifeSeptember 14, 2023Jingxuan Li, Chunlan Yang, Alexandre Jousset, Keming Yang, Xiaofang Wang, Zhihui Xu, Tianjie Yang, Xinlan Mei, Zengtao Zhong, Yangchun Xu, et al.gap), and after 1–3 min incubation at room temperature, exposed to a single electrical pulse using a MicroPulser Electroporator (Bio-Rad Laboratories) at field strength of 7.5 kV cm–1 for 4.5–6 ms. Immediately after the electrical

(15) Salmonella enterica relies on carbon metabolism to adapt to agricultural environmentsFrontiers in MicrobiologySeptember 7, 2023Min Han, Jasper Schierstaedt, Yongming Duan, Monika Nietschke, Sven Jechalke, Jacqueline Wolf, Michael Hensel, Meina Neumann-Schaal, Adam SchikoraaceBAK and PaceE aceEF extracted from E.coli NEB5α were electroporated using the Ec2 program in the MicroPulser Electroporator (Bio-Rad, Germany) into ΔaceB and ΔaceE competent cells, respectively. After 1 h of recovery in SOC

(16) A portable regulatory RNA array design enables tunable and complex regulation across diverse bacteriaNature CommunicationsAugust 29, 2023Baiyang Liu, Christian Cuba Samaniego, Matthew R. Bennett, Elisa Franco, James Chappellovernight-cultured cells 3 times with 10% glycerol. Plasmids were electroporated into these cells using a MicroPulser Electroporator (Bio-rad) with 1.2 kV, 1.25 kV, or 2.5 kV pulse. The cells were then recovered at 30 °C in LB without

(17) Non-IgE-reactive allergen peptides deteriorate the skin barrier in house dust mite-sensitized atopic dermatitis patientsFrontiers in Cell and Developmental BiologyAugust 22, 2023Karin Pfisterer, Matthias Wielscher, David Samardzic, Pauline Weinzettl, Dorte Symmank, Lisa E. Shaw, Raffaela Campana, Huey-Jy Huang, Matthias Farlik, Christine Bangert, et al.ClearColi™ BL21 (DE3) electrocompetent cells (Lucigen, Wisconsin, United States) by electroporation using a MicroPulser Electroporator (program Ec2: 0.2-cm cuvette and 2.5 kV) (Bio-Rad, United States). Expression of Der p 2 rec was induced

(18) Tigecycline Resistance-Associated Mutations in the MepA Efflux Pump in Staphylococcus aureusMicrobiology SpectrumAugust 17, 2023Honghao Huang, Peng Wan, Xinyue Luo, Yixing Lu, Xiaoshen Li, Wenguang Xiong, Zhenling Zengsolution was added to 1 μg of DNA and incubated on ice for 15 min. Electroporation was performed using the MicroPulser electroporator (Bio-Rad, Hercules, USA) with the following parameters: 2.5 kV, 200 ohms, and 25 μF. Electroporated

(19) Single-cell mutation rate of turnip crinkle virus (-)-strand replication intermediatesPLoS PathogAugust 14, 2023Camila Perdoncini Carvalho, Junping Han, Khwannarin Khemsom, Ruifan Ren, Luis Eduardo Aranha Camargo, Shuhei Miyashita, Feng Quelectrocompetent A. tumefaciens strain C58C1 via electroporation using the AGR setting of a Bio-Rad Micropulser Electroporator. Briefly, 5 μl of the plasmid DNA was mixed with 40 μl of agro cells and maintained on ice until electroporation.

(20) Synthetic Biology Toolkit for a New Species of Pseudomonas Promissory for Electricity Generation in Microbial Fuel CellsMicroorganismsAugust 9, 2023Franciene Rabiço, Matheus Pedrino, Julia Pereira Narcizo, Adalgisa Rodrigues de Andrade, Valeria Reginatto, María-Eugenia Guazzaronioccurred immediately. A 50 μL volume of electrocompetent cells was transformed with 2 ng of plasmid in a MicroPulser Electroporator (BioRad, Sao Paulo, Brazil) using program Ec1 (V = 1.8 kV) and 0.1 mm cuvettes. After electroporation,

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