Gene Pulser Xcell Microbial System from Bio-Rad

Product Specs
ItemGene Pulser Xcell Microbial System
CompanyBio-Rad
Price Get Pricing Get Quote for this Product
Catalog Number1652662
Brand/TrademarkBio-Rad Laboratories, Life Science Group
QuantityEA
ApplicationsElectroporation of bacteria and fungi, as well as other applications
Dimensions(WxDxH) 31 x 30 x 14 cm (12.2 x 11.8 x 5.51 inches)
Voltage200 to 3000 V
Cell TypeBacteria / Fungi
Weight6.6 kg
MethodElectroporation

Specifications/Features

Pulse Voltage:
200–3,000 V

Capacitance:
10, 25, 50 degrees F

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) N-acetylcysteine prevents catheter occlusion and inflammation in catheter associated-urinary tract infections by suppressing urease activityFrontiers in Cellular and Infection MicrobiologyOctober 26, 2023Arthika Manoharan, Jessica Farrell, Vina R. Aldilla, Greg Whiteley, Erik Kriel, Trevor Glasbey, Naresh Kumar, Kate H. Moore, Jim Manos, Theerthankar Dasan electroporation cuvette and subjected to 2500 V, 25 µF, and 200 Ω of current for electroporation (Gene Pulser Xcell Microbial System, BIO-RAD, Hercules, CA, USA) followed by incubation for 1 h at 37°C at 150 rpm. Following this, the

(2) High-Efficiency Transformation and Expression of Genomic Libraries in YeastMethods and ProtocolsSeptember 21, 2023Mira Loock, Luiza Berenguer Antunes, Rhiannon T Heslop, Antonio Alfonso De Lauri, Andressa Brito Lira, Igor CestariV1-200 (VWR, catalogue number 634-6000);Gene Pulser Xcell Microbial System (Bio-Rad, catalogue number 1652662);Vortex mixer (Fisher Scientific, catalogue number 02215365);Freezer −80 °C (New Brunswick™, Edison,

(3) Divergent proinflammatory immune responses associated with the differential susceptibility of cattle breeds to tuberculosisFrontiers in ImmunologySeptember 19, 2023Rishi Kumar, Sripratyusha Gandham, Avi Rana, Hemanta Kumar Maity, Uttam Sarkar, Bappaditya Dey25uF capacitance, and 1000 Ω resistance using an electroporator (Gene Pulser Xcell Microbial System #1652662, Bio-Rad). The cells were immediately aspirated and inoculated in 2ml of MB-7H9 broth without any selective

(4) An in vitro CRISPR-Cas12a-mediated protocol for direct cloning of large DNA fragmentsSTAR ProtocolsSeptember 1, 2023Xiaoqian Zeng, Shuliu Wang, Mindong Liang, Weishan Wang, Yue Jiang, Fei Xu, Leshi Liu, Hao Yan, Yaojun Tong, Lixin Zhang, et al.pulsed field gel electrophoresis (PFGE) with GenePulser Xcell™ microbial system (Bio-Rad, Catalog# 1652662) to make sure that the target DNA fragment had been cleaved (Figure 4B). MidRange PFG Marker (NEB, Catalog#

(5) Single cell susceptibility to SARS-CoV-2 infection is driven by variable cell statesbioRxivJuly 13, 2023Sam Reffsin, Jesse Miller, Kasirajan Ayyanathan, Margaret C. Dunagin, Naveen Jain, David C. Schultz, Sara Cherry, Arjun Rajplasmid into Endura electrocompetent E. coli cells (Lucigen #60242-1) using a Gene Pulser Xcell (Bio-Rad #1652662), allowing for recovery before plating serial dilutions and seeding cultures (200 mL each) for maxipreparation.

(6) Establishment of CRISPR-Cas9 system in Bifidobacteria animalis AR668Microbial Cell FactoriesJune 12, 2023Jiao Li, Xin Song, Zhiqiang Xiong, Guangqiang Wang, Yongjun Xia, Yijin Yang, Lianzhong AiHercules, CA, USA). The cuvette was pulsed at various field strengths and parallel resistances using the Gene Pulser Xcell Microbial Electroporation System (Bio-Rad). Following the electroporation, 0.9 ml of BS broth (supplemented with

(7) Formate as a supplementary substrate facilitates sugar metabolism and solvent production by Clostridium beijerinckii NCIMB 8052Synthetic and Systems BiotechnologyJune 1, 2023Ran Zhao, Wenyue Dong, Chen Yang, Weihong Jiang, Jinzhong Tian, Yang Guplaced in a 2 mm-gap electroporation cuvette (Harvard Apparatus, MA, USA) and immediately pulsed using a Gene Pulser Xcell microbial electroporation system (Bio-Rad, USA) with parameters set at 1.8 kV, 200 Ω and 25 μF. Next, the cells

(8) A genome-wide CRISPR screen maps endogenous regulators of PPARG gene expression in bladder canceriScienceMay 19, 2023Davide Tortora, Morgan E. Roberts, Gunjan Kumar, Sudha S. Kotapalli, Elie Ritch, Joshua M. Scurll, Brian McConeghy, Sunita Sinha, Alexander W. Wyatt, Peter C. Black, et al.of 100 μL STBL4 electrocompetent cells (Thermo Scientific). Electroporation was performed using the Gene Pulser Xcell Microbial System (Bio-Rad), in 25 μl aliquots at 1.8kV, and the mix was then immediately transferred to 1mL of pre-warmed

(9) Retrospective identification of intrinsic factors that mark pluripotency potential in rare somatic cellsbioRxiv : the preprint server for biologyFebruary 10, 2023Jain N, Goyal Y, Dunagin MC, Cote CJ, Mellis IA, Emert B, Jiang CL, Dardani IP, Reffsin S, Raj A.plasmid into Endura electrocompetent E. coli cells (Lucigen #60242–1) using a Gene Pulser Xcell (Bio-Rad #1652662), allowing for recovery before plating serial dilutions and seeding cultures (200 mL each) for maxipreparation.

(10) Role of EmaSR in Ethanol Metabolism by Acinetobacter baumanniiInternational Journal of Molecular SciencesOctober 20, 2022Hung-Yu Shu, Yu-Wen Huang, Ping-Yi Tsai, Kun-Sheng Hsieh, Guang-Huey Lintransferred into an electroporation cuvette (Hi-Lab Bioscience Co., Taichung, Taiwan), after which the Gene Pulser Xcell Microbial System (Bio-Rad, Taipei, Taiwan) was applied for electroporation at 1.8 kV. The resulting transformants were

(11) Compatibility rules of human enhancer and promoter sequencesNatureJuly 7, 2022Drew T. Bergman, Thouis R. Jones, Vincent Liu, Judhajeet Ray, Evelyn Jagoda, Layla Siraj, Helen Y. Kang, Joseph Nasser, Michael Kane, Antonio Rios, et al.libraries into Lucigen Endura Electrocompetent cells (60242) using 0.1cm cuvettes (BioRad) using the Gene Pulser Xcell Microbial System (BioRad) (10 uF, 600 Ohms, 1800 Volts) following the manufacturer’s recommendations. We expanded the

(12) Spinning sugars in antigen biosynthesis: characterization of the Coxiella burnetii and Streptomyces griseus TDP-sugar epimerasesJournal of Biological ChemistryMay 1, 2022Alice R. Cross, Sumita Roy, Mirella Vivoli Vega, Martin Rejzek, Sergey A. Nepogodiev, Matthew Cliff, Debbie Salmon, Michail N. Isupov, Robert A. Field, Joann L. Prior, et al.Scientific #5510-11). A single pulse of 1.8 kV was given by a Gene Pulser Xcell Microbial System (Bio-Rad #1652662). Cells were recovered by adding 1000 μl prewarmed LB medium. The complemented cell suspension was incubated

(13) Pooled genetic perturbation screens with image-based phenotypesNature ProtocolsFebruary 1, 2022David Feldman, Luke Funk, Anna Le, Rebecca J. Carlson, Michael D. Leiken, FuNien Tsai, Brian Soong, Avtar Singh, Paul C. BlaineyElectroporation cuvettes (BioRad, cat. no. 165-2093) Gene Pulser Xcell Microbial System (Bio-Rad, cat. no. 1652662) Nunc EasYFlask 225 cm2, filter cap, 70-mL working volume (T225 flask; Thermo Fisher Scientific, cat.

(14) Efficient Generation of P53 Biallelic Mutations in Diannan Miniature Pigs Using RNA-Guided Base EditingLife (Basel)December 17, 2021Honghui Li et al.pGL3-U6-P53-sgRNA-Exon5/Exon4 plasmids (10.5 μg) were transfected by electroporation at 250 V for a single 20 ms pulse (Gene PulserXcell Microbial System, Bio-Rad, Hercules, CA, USA) in a 4mm gap cuvette. Then, the cells were seeded into 5 mL of fresh

(15) XACT-seq: A photocrosslinking-based technique for detection of the RNA polymerase active-center position relative to DNA in Escherichia coliSTAR ProtocolsDecember 17, 2021Chirangini Pukhrambam, Irina O. Vvedenskaya, Bryce E. Nickels(2020),https://github.com/NickelsLabRutgers/XACT-seq ,, Other,Other,Other ,, MicroPulser Electroporator,Bio-Rad,Cat#1652662 Electroporation cuvettes 1 mm gap,VWR,Cat#58017-890 Cel-Gro Tissue Culture Rotator,Thermo Fisher Scientific,N/A Rayonet

(16) Engineering a genome‐reduced bacterium to eliminate Staphylococcus aureus biofilms in vivoMolecular Systems BiologyOctober 6, 2021Victoria Garrido, Carlos Piñero‐Lambea, Irene Rodriguez‐Arce, Bernhard Paetzold, Tony Ferrar, Marc Weber, Eva Garcia‐Ramallo, Carolina Gallo, María Collantes, Iván Peñuelas, et al.Scientific, DynabeadsTM MyOneTM Streptavidin C1,Invitrogen,65001 "Electroporation system, Gene Pulser X cell",Bio‐Rad,1652662 Electroporation cuvettes 0.1 cm,Bio‐Rad,1652089 ELISA plates reader‐ IdAB,Labsystem,Multiskan Ex "Filtropur

(17) Highly Selective, CRISPR/Cas9‐Mediated Isolation of Genes and Genomic Loci from Complex Genomes by TAR Cloning in YeastCurrent ProtocolsAugust 9, 2021Natalay Kouprina, Jung‐Hyun Kim, Vladimir LarionovAXYT400RS)6‐cc luer‐lock syringe (PRACTICON, cat. no.70103232)Gene Pulser and cuvettes (BioRad, cat. no. 1652662)ToothpicksGeneRuler 1 kb plus DNA ladder (Thermo Fisher Scientific, cat. no. SM1331)0.2‐ml PCR strip

(18) N-Acetylcysteine Protects Bladder Epithelial Cells from Bacterial Invasion and Displays Antibiofilm Activity against Urinary Tract Bacterial PathogensAntibiotics (Basel)July 23, 2021Arthika Manoharan, Samantha Ognenovska, Denis Paino, Greg Whiteley, Trevor Glasbey, Frederik H. Kriel, Jessica Farrell, Kate H. Moore, Jim Manos, Theerthankar Daselectroporation cuvette. The mixture was subjected to 2500 V, 25 µF, and 200 Ω on the electroporator (Gene Pulser Xcell Microbial System, BIO-RAD, Hercules, CA, USA), and then incubated for 1 h at 37 °C under dynamic conditions. Following

(19) Scalable characterization of the PAM requirements of CRISPR–Cas enzymes using HT-PAMDANature ProtocolsMarch 1, 2021Russell T. Walton, Jonathan Y. Hsu, J. Keith Joung, Benjamin P. KleinstiverCell culture CO2 incubator Magnetic stir plate Gene Pulser Xcell Microbial System (BioRad, cat. no. 1652662) Vortexer Labnet mini plate spinner (Thomas Scientific, cat. no. 1225Z37) Microcentrifuge (Eppendorf,

(20) Cloning and Heterologous Expression of the ¥ -Galactosidase Gene from Bifidobacterium longum RD47 in B. bifidum BGN4Journal of Microbiology and BiotechnologyNovember 28, 2019Min Ju Park, Myeong Soo Park, Geun Eog JiE. coli via heat-shock and CaCl2 method and introduced into B. bifidum BGN4 by electroporation with the Gene Pulser Xcell Microbial Electroporation System (Bio-Rad, USA). The positive colonies were isolated, and the plasmids were purified

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