EcoRI from Promega

Product Specs
ItemEcoRI
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberR6011
Quantity5,000u
Concentration12u/µl

Sequence

G▼AATT C
C TTAA▲G

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) DONSON facilitates Cdc45 and GINS chromatin association and is essential for DNA replication initiationNucleic Acids ResearchOctober 13, 2023Georgia Kingsley, Aggeliki Skagia, Paolo Passaretti, Cyntia Fernandez-Cuesta, Alicja Reynolds-Winczura, Kinga Koscielniak, Agnieszka Gambusin DMSO at 8 mM and added to the extract along with demembranated sperm chromatin at 40 μM. EcoR1 (R6011, Promega) was purchased at stock 12 U/μl and added to the extract at 0.05 U/μl. Mitomycin C (Calbiochem)

(2) TRAIP resolves DNA replication-transcription conflicts during the S-phase of unperturbed cellsNature communicationsAugust 21, 2023Scaramuzza S, Jones RM, Sadurni MM, Reynolds-Winczura A, Poovathumkadavil D, Farrell A, Natsume T, Rojas P, Cuesta CF, Kanemaki MT, et al.Committee and by UK home office project license no: P081C27D8. Inhibitors and recombinant proteins EcoR1 (R6011, Promega) was purchased at stock 12 U/µl and added to the extract at 0.05 U/µl, Aphidicolin (A0781,

(3) Modeling the Orthosteric Binding Site of the G Protein-Coupled Odorant Receptor OR5K1Journal of Chemical Information and ModelingApril 10, 2023Alessandro Nicoli, Franziska Haag, Patrick Marcinek, Ruiming He, Johanna Kreißl, Jörg Stein, Alessandro Marchetto, Andreas Dunkel, Thomas Hofmann, Dietmar Krautwurst, et al.into expression vectors was done with T4-DNA ligase (#M1804, Promega, Madison, USA) via EcoRI/NotI (#R6017/#R6435, Promega, Madison, USA) into the expression plasmid pFN210A100 and verified by Sanger sequencing

(4) The human odorant receptor OR10A6 is tuned to the pheromone of the commensal fruit fly Drosophila melanogasteriScienceNovember 18, 2022Tim Frey, Charles A. Kwadha, Franziska Haag, Julien Pelletier, Erika A. Wallin, Elsa Holgersson, Erik HedenstrÃ¶m, BjÃ¶rn Bohman, Marie Bengtsson, Paul G. Becher, et al.Madison, USA) either MfeI/NotI (#R3589S/ # R0189S, New England Biolabs, Ipswich, UK) or EcoRI/NotI (#R6017/ #R6435, Promega, Madison, USA) into the expression plasmid (#pFN210A SS-HaloTag® CMV-neo Flexi®-Vector,

(5) The p97 segregase cofactor Ubxn7 facilitates replisome disassembly during S-phaseThe Journal of biological chemistryAugust 1, 2022Tarcan Z, Poovathumkadavil D, Skagia A, Gambus A.dissolved in DMSO at 8 mM and added to the extract along with demembranated sperm nuclei at 40 μM. EcoR1 (R6011, Promega) was purchased at stock 12 U/μl and added to the extract at 0.05 U/μl. Recombinant proteins Recombinant

(6) Targeting SNORA38B attenuates tumorigenesis and sensitizes immune checkpoint blockade in non-small cell lung cancer by remodeling the tumor microenvironment via regulation of GAB2/AKT/mTOR signaling pathwayJournal for ImmunoTherapy of CancerMay 16, 2022Yue Zhuo et al.post LLC inoculation by i.p. every 4 days. RNA pull-down assays Restriction endonucleases EcoRI (R6011, Promega) and SalI (R6051, Promega) were used to linearized the pGEM-3Z vector (P2151, Promega), then

(7) Copper-mediated thiol potentiation and mutagenesis-guided modeling suggest a highly conserved copper-binding motif in human OR2M3Cellular and Molecular Life SciencesJune 1, 2020Franziska Haag, Lucky Ahmed, Krystle Reiss, Eric Block, Victor S. Batista, Dietmar Krautwurstfor OR2M3 and OR1A1 (#R0589S/#R0189S, New England BioLabs, Ipswich, USA) or EcoRI/NotI for OR2W1 (#R6017/#R6435, Promega, Madison, USA) into the expression plasmid pI2-dk(39aa-rho-tag) (aa, amino acid) [53,

(8) An intestinal zinc sensor regulates food intake and developmental growthNatureApril 1, 2020Redhai S, Pilgrim C, Gaspar P, Giesen LV, Lopes T, Riabinina O, Grenier T, Milona A, Chanana B, Swadling JB, et al.Hodor F and Hodor R (see below). The PCR product was digested with NotI and EcoRI (Promega #R6435 and #R6017 respectively) and cloned into the pUASTattB vector53. hodor-containing pUASTattB was amplified, purified

(9) Evaluation of bisulfite kits for DNA methylation profiling in terms of DNA fragmentation and DNA recovery using digital PCRPLOS ONEJune 14, 2018Sam Kint, Ward De Spiegelaere, Jonas De Kesel, Linos Vandekerckhove, Wim Van CriekingeMoreover, 8.65 μl of untreated DNA samples from all donors was restricted with EcoRI enzyme (Promega, R6011) according to the manufacturer’s instructions. 2μl of this restricted DNA was also used for quantification

(10) IL-6-HaloTag® enables live-cell plasma membrane staining, flow cytometry, functional expression, and de-orphaning of recombinant odorant receptorsJournal of Biological MethodsNovember 3, 2017Franziska Noe, Tim Frey, Julia Fiedler, Christiane Geithe, Bettina Nowak, Dietmar Krautwurstproducts were purified (gel extraction kit, #28706, Qiagen, Hilden, Germany), digested either EcoRI/NotI (#R6017/ #R6435, Promega, Madison, USA) or MfeI/NotI (#R0589S/ #R0189S, New England Biolabs, Ipswich, USA) and

(11) The genetic audiogenic seizure hamster from Salamanca: The GASH:SalEpilepsy & BehaviorJune 1, 2017Luis J. Muñoz, Melissa M. Carballosa-Gautam, Kira Yanowsky, Natividad García-Atarés, Dolores E. Lópezfollowing: 1× REact I buffer (#Y90002, Invitrogen, Carlsbad, CA, USA), EcoRI restriction enzyme (3.6 U) (#R6011, Promega, Madison, WI, USA), and MseI restriction enzyme (4.0 U) (#15494-016, Invitrogen). Following

(12) Sterol O-Acyltransferase 2 Contributes to the Yolk Cholesterol Trafficking during Zebrafish EmbryogenesisPLOS ONEDecember 9, 2016Nai-Yun Chang, Yen-Ju Chan, Shih-Torng Ding, Yen-Hua Lee, Wei-Chun HuangFu, I-Hsuan Liu

(13) Quorum Sensing Controls Swarming Motility of Burkholderia glumae through Regulation of RhamnolipidsPLOS ONEJune 5, 2015Arvin Nickzad, François Lépine, Eric Déziel

(14) Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobiaBMC Plant BiologyDecember 1, 2014Araceli Nora García, Nicolás Daniel Ayub, Ana Romina Fox, María Cristina Gómez, María José Diéguez, Elba María Pagano, Carolina Andrea Berini, Jorge Prometeo Muschietti, Gabriela Sotoexpressing MsSN1, plasmid pSJ33-MsSN1 carrying MsSN1 was constructed by subcloning the 0.5-kb EcoRI (Cat. # R6011, Promega) fragment from pCR2.1TOPO-MsSN1 into pSJ33 [28], and afterward introduced in Escherichia coli

(15) Characterization of Persistent Virus-Like Particles in Two Acetate-Fed Methanogenic ReactorsPLoS ONENovember 22, 2013I-Chieh Chien, John Scott Meschke, Heidi L. Gough, John F. Fergusonspectrophotometer (NanoDrop Technologies, Wilmington, DE). Plasmids were linearized by restriction enzyme EcoRI (R6011, Promega Co., Madison, WI). Reaction mixes contained 2 μl DNA templates, 4.5 μl RealMasterMix and individual

(16) High-Yield Production in Escherichia coli of Fungal Immunomodulatory Protein Isolated from Flammulina velutipes and Its Bioactivity Assay in VivoInternational Journal of Molecular SciencesJanuary 24, 2013Xianghui Kong, Jiechi Zhang, Xue Han, Piqi Zhang, Xiaodong Dai, Jianing Liu, Xinxin Zhang, Imshik Lee, Shenkui LiuPCR product was gel purified and then ligated into pET30a (+) at the BamHI (R602A, Promega) and EcoRI (R6011, Promega) sites. The obtained plasmid was identified by DNA sequencing and named pET30a-FIP-fve. 3.3.

(17) Ocorrência de DNA de herpesvírus bovino tipo 4 em gado Holstein argentino de Santiago del Estero, ArgentinaBrazilian Journal of Veterinary Research and Animal ScienceDecember 1, 2011Sandra Elizabeth Pérez, Andrea Elizabeth Verna, María Rosa Leunda, Paula Ariela Favier, María Carolina Ceriani, Pedro Edgardo Morán, Anselmo Carlos Odeón, Eduardo Néstor EstebanQiagen). DNA obtained from 13 PBL samples was digested with the restriction enzyme EcoRI (Promega, Cat. R6011), according to the manufacturer’s instructions. Digestion products were separated by electrophoresis

(18) RDH10 is the primary enzyme responsible for the first step of embryonic Vitamin A metabolism and retinoic acid synthesisDevelopmental BiologySeptember 15, 2011Krysten M. Farjo, Gennadiy Moiseyev, Olga Nikolaeva, Lisa L. Sandell, Paul A. Trainor, Jian-xing Ma

(19) Mycoplasma Bovis Outbreak in a Herd of North American Bison (Bison Bison)Journal of Veterinary Diagnostic InvestigationSeptember 3, 2010Kyathanahalli S. Janardhan, Mike Hays, Neil Dyer, Richard D. Oberst, Brad M. DeBeyNew England Biolabs Inc., Ipswich, MA. i. Catalog no. 1161-1127, Bio-Rad Laboratories, Hercules, CA. j. Catalog no. R6011, Promega Corp., Madison, WI. k. Catalog no. R6381, Promega Corp., Madison, WI. l. Catalog no. R6021,

(20) Inactivation of Burkholderia cepacia Complex Phage KS9 gp41 Identifies the Phage Repressor and Generates Lytic VirionsJournal of VirologyFebruary 1, 2010Karlene H. Lynch, Kimberley D. Seed, Paul Stothard, Jonathan J. Dennis

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EcoRI

Description

Sequence

Promega

Citations (24)

Reviews

EcoRI from Promega

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