High Pure PCR Product Purification Kit from Roche

Product Specs
ItemHigh Pure PCR Product Purification Kit
CompanyRoche
Price Get Pricing Get Quote for this Product
Catalog Number11732668001
RangeThe synergistic process requires a minimum fragment length of approximately 100 bp
Quantity1 kit (50 purifications)
SourceReaction
Nucleic Acid TypeDNA Fragment, PCR Product

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Roche

Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road

Indianapolis, Indiana

United States

Phone: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911

Company Profile

Website: sequencing.roche.com

(1) Comparison of an automated DNA extraction and 16S rDNA real time PCR/sequencing diagnostic method using optimized reagents with culture during a 15-month study using specimens from sterile body sitesBMC MicrobiologyDecember 1, 2022Konrad Egli, Martin Risch, Lorenz Risch, Thomas Bodmerinhibition. Purification of PCR products was performed with the High Pure PCR Product Purification Kit (order number 11732668001, product of Roche but supplied by Sigma Aldrich) according to the package insert. A maximum of 9.5 μL

(2) Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulansInternational Journal of Molecular SciencesSeptember 22, 2022Joanna Gawlik, Michal Koper, Albert Bogdanowicz, Piotr Weglenski, Agnieszka Dzikowskafragments were purified with a High Pure PCR Product Purification Kit (Roche Diagnostics, cat. no. 11732668001, Mannheim, Germany). Fragments for cloning were amplified using specific primers (Table S1). The Golden

(3) The human amniotic epithelium confers a bias to differentiate toward the neuroectoderm lineage in human embryonic stem cellseLifeJuly 11, 2022Ávila-González D, Portillo W, Barragán-Álvarez CP, Hernandez-Montes G, Flores-Garza E, Molina-Hernández A, Díaz-Martínez NE, Díaz NF.isolated using the High Pure FFPET DNA Isolation Kit according to the supplier’s instructions (Roche, 11732668001). The gDNA samples were sent to Germany (Cygenia, https://www.cygenia.com/) to analyze the DNA methylation

(4) Cancer-Testis Gene Biomarkers Discovered in Colon Cancer PatientsGenes (Basel)May 1, 2022Mikhlid H. Almutairi, Turki M. Alrubie, Abdullah M. Alamri, Bader O. Almutairi, Abdulwahed F. Alrefaei, Maha M. Arafah, Mohammad Alanazi, Abdelhabib Semlaliproducts were then purified using the Roche Applied Science High Pure PCR Product Purification Kit (Roche; 11732668001, Darmstadt, Germany). Then, 10 ng/μL of DNA in a total volume of 10 μL was placed in a clean 1.5 mL

(5) 锁核苷酸PCR解析植物内生细菌多样性方法BIO-PROTOCOLJanuary 1, 2021镇华于, Makoto Ikenaga, Masao Sakai, 光华王LLC. 1 www.bio-protocol.org/e2003525 4. DOI:10.21769/BioProtoc.2003525 High Pure PCR Product Purification Kit (Roche, Indianapolis IN, USA, catalog number: 11732668001) 5. PCR 相关试剂 (Takara RR030A Premix Ex Taq™ Hot Start Version, catalog number: RR030A) 6. LNA 扩增引物和细菌特异性扩增引物

(6) Molecular Epidemiology of Peste Des Petits Ruminants Cases Associated with Abortion in Sheep and Goat in Marmara Region of Turkey, 2018The Pakistan Veterinary JournalDecember 1, 2020Zuleyha Pestilagarose gel electrophoresis. The amplified RT-PCR products were purified using a High Pure PCR Product Purification Kit (Lot No. 11732668001, Roche, Germany) and sequenced using an ABI 3100 automatic sequencer. The sequences from both directions

(7) Identification of genomic insertion and flanking sequences of the transgenic drought-tolerant maize line “SbSNAC1-382” using the single-molecule real-time (SMRT) sequencing methodPLOS ONEApril 10, 2020Tingru Zeng, Dengfeng Zhang, Yongxiang Li, Chunhui Li, Xuyang Liu, Yunsu Shi, Yanchun Song, Yu Li, Tianyu Wang(Roche, Cat. No. 11585614910) and purified using the high-purity PCR product purification kit (Roche, No.11732668001). The DNA blots were pre-hybridized at 42˚C for 1 h in DIG easy hybgranules and then hybridized to denatured

(8) Increasing the catalytic power of the flavin reductase DszD enzyme using site-directed mutagenesis method in Rhodococcus erythropolisJournal of Arak University of Medical SciencesDecember 30, 2019Ramin Fallahzadeh, Kasra Esfahani, Abbas Akhavan Sepahi, Nasrin Kamali, Bijan Bambai،EcoRI، T4 Ligase‬کیت‬ ‫استخراج ژل آگارز (‪ )Cat. No. 11696505001‬و کیت تخلیص‬ ‫محصول ‪ )‌Cat. No. 11732668001) PCR‬از شرکت ‪Roche‬‬ ‫آلمان؛ کیت استخراج ‪ )‌Cat. No. K1014-100) DNA‬و کیت‬ ‫تخلیص پالسمید (‪ )Cat.

(9) Molecular Characterization of Aquaglyceroporine: A Novel Mutation in LmAQP1 from Leishmania major (MRHO/IR/75/ER)Iran J ParasitolNovember 2, 2019Gilda Eslami, Maryam Ghavami, Ali Reza Moradi, Hamid Nadri, Salman Ahmadiantriplicate. Sequencing The amplified fragments were purified using High Pure PCR Product Purification Kit (#11732668001, Roche) and sequenced. The sequencing was done triplicate with both forward and reverse primers. Characterization

(10) Evaluation of bisulfite kits for DNA methylation profiling in terms of DNA fragmentation and DNA recovery using digital PCRPLOS ONEJune 14, 2018Sam Kint, Ward De Spiegelaere, Jonas De Kesel, Linos Vandekerckhove, Wim Van Criekingekits were also analyzed. The amplicons were purified with the High Pure PCR Product Purification Kit (11732668001, Roche) and sequenced in a MiSeq sequencing system (Illumina). The sequencing reads were aligned using

(11) Evolutionary emergence of the rac3b / rfng / sgca regulatory cluster refined mechanisms for hindbrain boundaries formationProceedings of the National Academy of SciencesApril 17, 2018Joaquín Letelier et al.biological replicates were generated. These libraries were purified with a PCR Product Purification kit (No. 11732668001; Roche), their concentrations were measured using the Qubit dsDNA BR assay kit (No. Q32850; Molecular Probes

(12) Combined Magnetic Tweezers and Micro-mirror Total Internal Reflection Fluorescence Microscope for Single-Molecule Manipulation and VisualizationSingle Molecule AnalysisJanuary 1, 2018Yeonee Seol, Keir C. NeumanRestriction enzyme digestion BsaI-HF (R3535, NEB) and supplied buffer Products from PCR reactions DNA clean-up kit (11732668001, Roche) 2.1.5 DNA ligation T4 DNA ligase (M1804, Promega) and supplied buffer Products from restriction

(13) The mitochondrial genomes of the acoelomorph worms Paratomella rubra, Isodiametra pulchra and Archaphanostoma ylvaeScientific ReportsDecember 1, 2017Helen E. Robertson, FranÃ§ois Lapraz, Bernhard Egger, Maximilian J. Telford, Philipp H. Schifferwere purified using the High Pure PCR Product Purification Kit (Roche Applied Sciences: Product No. 11732668001) and sent for sequencing by Source BioScience Life Sciences. Only amplifications that resulted in one

(14) Risk of Myopathy in Patients in Therapy with Statins: Identification of Biological Markers in a Pilot StudyFrontiers in PharmacologyJuly 27, 2017Giulia M. Camerino et al.the pellet of bead were recuperated and purified with high pure PCR product purification kit (N.O. 11732668001 Roche) (Tricarico et al., 2008; Dinardo et al., 2012; Camerino et al., 2013). Real-time PCR was performed

(15) Detection and Genotyping of Human Papillomaviruses from Archival Formalin‐Fixed Tissue SamplesCurrent Protocols in MicrobiologyNovember 19, 2016Koenraad Van Doorslaer, Zigui Chen, Alison A. McBrideand vector. Materials PCR clean-up kit (e.g. High Pure PCR Product Purification Kit; Roche, cat.no. 11732668001) Agarose Gel purification kit (e.g. zymoclean Gel DNA recovery kit; Zymo. cat. no. D4007) 10X ThermoPol

(16) Single molecule measurements of DNA helicase activity with magnetic tweezers and t-test based step-finding analysisMethodsAugust 1, 2016Yeonee Seol, Marie-Paule Strub, Keir C. Neumansingle-stranded region (Fig. 2b). The digested products were purified using a PCR purification kit (11732668001, Roche). To complete the 174 bp DNA hairpin, the digestion product was ligated to a 12 bp hairpin DNA

(17) Introducing precise genetic modifications into human 3PN embryos by CRISPR/Cas-mediated genome editingJournal of Assisted Reproduction and GeneticsMay 1, 2016Xiangjin Kang, Wenyin He, Yuling Huang, Qian Yu, Yaoyong Chen, Xingcheng Gao, Xiaofang Sun, Yong Fancompletion of the WGA reaction, the products were purified using the HP PCR Product Purification Kit (no. 11732668001, Roche). Purified DNA (1 μl) was run on agarose gels to confirm successful amplification, and the remaining

(18) A single three-dimensional chromatin compartment in amphioxus indicates a stepwise evolution of vertebrate Hox bimodal regulationNature GeneticsMarch 1, 2016Rafael D Acemel et al.developmental stage). These libraries were purified with a High Pure PCR Product Purification Kit (Roche, 11732668001), their concentrations measured using the Quanti-iTTM PicoGreen dsDNA Assay Kit (Invitrogen, P11496)

(19) THE EFFECTS OF CRUDE RECOMBINANT VIRAL PROTEIN VACCINES AGAINST GROUPER SLEEPY DISEASE IRIDOVIRUS (GSDIV) ON HUMPBACK GROUPER (Cromileptes altivelis)Indonesian Aquaculture JournalDecember 31, 2015Ketut Mahardika, Indah Mastutiagarose gel 1.5 % in TAE 1x. PCR product was purified using a PCR purification kit (Roche, Cat. No. 11732668001) and visualized in agarose gel (1.5 % in TAE 1x) to get a purify and single band. The purificated DNA

(20) Enhancer of zeste acts as a major developmental regulator ofCiona intestinalisembryogenesisBiology OpenSeptember 15, 2015Emilie Le Goff, Camille Martinand-Mari, Marianne Martin, Jérôme Feuillard, Yvan Boublik, Nelly Godefroy, Paul Mangeat, Stephen Baghdiguian, Giacomo Cavallial., 2010). PCR products were sequenced and purified (High Pure PCR Product Purification Kit, Roche; 11732668001). Purified PCR product (300 ng) was used as template for the DIG RNA Labeling Kit (Roche; 11277073910)

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High Pure PCR Product Purification Kit from Roche

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High Pure PCR Template Preparation Kit From Roche

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