Description
The NEBNext™ End Repair Module has been optimized to repair DNA fragmented by nebulization, acoustic shearing, or nucleases. Fragmented DNA is converted by the NEBNext End Repair Module to blunt ended DNA having 5´-phosphates and 3´-hydroxyl. The NEBNext End Repair Module is provided as a master mix to maximize efficiency and convenience in DNA sample preparation workflows.
DNA repaired by the NEBNext End Repair Module can be subsequently converted to DNA having 3´ dA-tails with the NEBNext dA-tailing Module or used directly for blunt end cloning or blunt ended adaptor ligation.
The NEBNext End Repair Module has been validated by sequencing with the Illumina Genome Analyzer II in conjunction with the NEBNext dA-Tailing Module, NEBNext Quick Ligation Module and Phusion™ High-Fidelity PCR Master Mix.
The NEBNext End Repair Module has also been validated by sequencing with 454 GS FLX Titanium in conjunction with the NEBNext Quick Ligation Module and the NEBNext Adapter Fill-in and ssDNA Isolation Module.